Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,663,950	T→C	R223R (CGA→CGG)	gadW ←	DNA‑binding transcriptional dual regulator GadW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,663,950	0	T	C	100.0%	259.2 / NA	71	R223R (CGA→CGG)	gadW	DNA‑binding transcriptional dual regulator GadW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/45);  total (26/45)

CGGCAGAAATCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGG  >  NC_000913/3663815‑3664086                                                                                                                                        |                                                                                                                                         cGGCAGAAATCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAGTATGTAAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGaa                                                                                                                                      <  2:215194/140‑1 (MQ=255)  ggCAGAAATCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGaaa                                                                                                                                     <  1:1093647/140‑1 (MQ=255)  ggCAGAAATCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGaaa                                                                                                                                     <  2:623765/140‑1 (MQ=255)  ggCAGAAATCATCAGGGAAGACTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATACGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGaaa                                                                                                                                     <  2:1059157/140‑1 (MQ=255)       aaaTCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATgtgt                                                                                                                                <  2:427846/140‑1 (MQ=255)         atcatcAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGttt                                                                                                                              >  1:519934/1‑140 (MQ=255)            atcaGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTtata                                                                                                                           <  1:651357/140‑1 (MQ=255)            atcaGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTtata                                                                                                                           <  1:999169/140‑1 (MQ=255)                 ggAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTa                                                                                                                      <  2:87491/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGTCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:1018590/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:227009/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:403423/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:516012/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:572788/140‑1 (MQ=255)                   aaGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACg                                                                                                                    <  1:710615/140‑1 (MQ=255)                        tttCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTa                                                                                                                      <  1:256794/133‑1 (MQ=255)                        tttCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTa                                                                                                                      >  2:256794/1‑133 (MQ=255)                        tttCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTa                                                                                                               <  2:956731/140‑1 (MQ=255)                           cacaTGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTtat                                                                                                                            <  1:29168/124‑1 (MQ=255)                           cacaTGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTtat                                                                                                                            >  2:29168/1‑124 (MQ=255)                           cacaTGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTa                                                                                                                      <  2:696153/130‑1 (MQ=255)                           cacaTGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTa                                                                                                                      >  1:696153/1‑130 (MQ=255)                           cacaTGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTg                                                                                                            >  1:92085/1‑140 (MQ=255)                                 aaGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAg                                                                                                      <  2:13570/140‑1 (MQ=255)                                     aGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCcac                                                                                                  >  1:620319/1‑140 (MQ=255)                                      ggTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCcaca                                                                                                 >  2:885754/1‑140 (MQ=255)                                        tgtgAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTa                                                                                                               >  1:626169/1‑124 (MQ=255)                                        tgtgAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTa                                                                                                               <  2:626169/124‑1 (MQ=255)                                        tgtgAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACAtt                                                                                               >  1:515434/1‑140 (MQ=255)                                         gtgAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACAttt                                                                                              <  2:515434/140‑1 (MQ=255)                                          tgAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACAtttt                                                                                             >  2:291333/1‑140 (MQ=255)                                            agaTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACAttttc                                                                                            <  2:500870/139‑2 (MQ=255)                                            agaTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACAttttc                                                                                            >  1:500870/1‑138 (MQ=255)                                            agaTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTc                                                                                           >  1:169854/1‑140 (MQ=255)                                            agaTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTc                                                                                           >  2:1104013/1‑140 (MQ=255)                                              aTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCg                                                                                         <  1:1097088/140‑1 (MQ=255)                                              aTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCg                                                                                         <  2:827325/140‑1 (MQ=255)                                              aTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCg                                                                                         <  1:1093788/140‑1 (MQ=255)                                              aTCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCg                                                                                         <  1:731501/140‑1 (MQ=255)                                                ccTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCa                                                                                       <  2:519934/140‑1 (MQ=255)                                                ccTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCa                                                                                       <  1:207734/140‑1 (MQ=255)                                                 cTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCaa                                                                                      >  1:318749/1‑140 (MQ=255)                                                 cTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTAGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCaa                                                                                      >  2:961751/1‑140 (MQ=255)                                                  tGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAAt                                                                                     <  2:318749/140‑1 (MQ=255)                                                   gACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATa                                                                                    <  2:62598/140‑1 (MQ=255)                                                   gACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATa                                                                                    <  2:475338/140‑1 (MQ=255)                                                    aCCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAg                                                                                   >  1:591142/1‑140 (MQ=255)                                                     ccAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGt                                                                                  >  2:184253/1‑140 (MQ=255)                                                                aTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGaa                                                                       >  2:493021/1‑140 (MQ=255)                                                                   cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATg                                                                               >  2:534968/1‑129 (MQ=255)                                                                   cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATg                                                                               <  1:534968/129‑1 (MQ=255)                                                                   cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAAttt                                                                    >  1:936171/1‑140 (MQ=255)                                                                     aaaTATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGa                                                                  >  1:795255/1‑140 (MQ=255)                                                                         atGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTa                                                              <  2:936171/140‑1 (MQ=255)                                                                                      gTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCg                                                 >  1:244306/1‑140 (MQ=255)                                                                                        aCCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTc                                               <  2:244306/140‑1 (MQ=255)                                                                                        aCCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTc                                               <  2:74423/140‑1 (MQ=255)                                                                                          cTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTg                                             >  1:420925/1‑140 (MQ=255)                                                                                           tGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTgg                                            >  2:274566/1‑140 (MQ=255)                                                                                                aaTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATc                                       >  1:485954/1‑140 (MQ=255)                                                                                                 aTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCg                                      >  1:78156/1‑140 (MQ=255)                                                                                                  tGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGa                                     <  2:92085/140‑1 (MQ=255)                                                                                                  tGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGa                                     <  2:78156/140‑1 (MQ=255)                                                                                                    ttGCGCAAACTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACa                                   <  1:1104013/140‑1 (MQ=255)                                                                                                            aCTGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATATCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAg                           <  2:925497/140‑1 (MQ=255)                                                                                                              tGATGTGGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGc                         <  1:805840/140‑1 (MQ=255)                                                                                                                 tgtgGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAg                      <  1:291333/140‑1 (MQ=255)                                                                                                                   tgGCGTTACACCATAATATTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTa                    <  1:925499/140‑1 (MQ=255)                                                                                                                                 aataTTGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa      <  1:885754/140‑1 (MQ=255)                                                                                                                                     ttGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAgg  <  2:795255/140‑1 (MQ=255)                                                                                                                                     ttGCCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAgg  <  1:961751/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGGCAGAAATCATCAGGGAAGAGTTTCACATGAAGCAGGTGTGAGATCCTGACCAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGG  >  NC_000913/3663815‑3664086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑