Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,122,079	C→T	G70D (GGC→GAC)	hslV ←	peptidase component of the HslVU protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,122,079	0	C	T	100.0%	287.7 / NA	83	G70D (GGC→GAC)	hslV	peptidase component of the HslVU protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/45);  total (38/45)

GCTGCACCACGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGCCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTA  >  NC_000913/4121942‑4122213                                                                                                                                          |                                                                                                                                       gCTGCACCACGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGtcc                                                                                                                                      <  2:812891/140‑1 (MQ=255)    gCACCACGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGa                                                                                                                                   >  1:840322/1‑140 (MQ=255)     caccacGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGAt                                                                                                                                  <  1:1081646/140‑1 (MQ=255)          cGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCAtt                                                                                                                             >  2:150002/1‑140 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGTTTCAAGTTTTCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:1042031/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:840322/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:1147193/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:448045/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:1006264/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  2:459821/140‑1 (MQ=255)                 gTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGtt                                                                                                                      <  1:226064/140‑1 (MQ=255)                    aCCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTAc                                                                                                                   >  1:394140/1‑140 (MQ=255)                          gatgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaa                                                                                                             <  1:677493/140‑1 (MQ=255)                          gatgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaa                                                                                                             <  1:831125/140‑1 (MQ=255)                           atgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaaa                                                                                                            >  2:480211/1‑140 (MQ=255)                           atgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaaa                                                                                                            >  1:902320/1‑140 (MQ=255)                            tgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGTGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAAc                                                                                                           >  1:81561/1‑140 (MQ=255)                            tgatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGCGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTCGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAAc                                                                                                           >  2:1019992/1‑140 (MQ=255)                             gatAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACa                                                                                                          >  2:357366/1‑140 (MQ=255)                                  gCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCg                                                                                                     <  1:516892/140‑1 (MQ=255)                                     aTGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGGTCAAACAGTTCGAAc                                                                                                  >  1:215543/1‑140 (MQ=255)                                      tGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACa                                                                                                 >  1:754602/1‑140 (MQ=255)                                      tGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACa                                                                                                 >  1:1147363/1‑140 (MQ=255)                                      tGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACa                                                                                                 >  2:596301/1‑140 (MQ=255)                                       gCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAg                                                                                                >  1:313728/1‑140 (MQ=255)                                          gTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaa                                                                                                             <  2:323442/124‑1 (MQ=255)                                          gTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCaa                                                                                                             >  1:323442/1‑124 (MQ=255)                                           tttCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTa                                                                                            >  2:565803/1‑140 (MQ=255)                                             tCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTaaa                                                                                          <  2:401712/140‑1 (MQ=255)                                                tCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGTGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGc                                                                                       <  2:81561/140‑1 (MQ=255)                                                tCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGc                                                                                       >  1:932719/1‑140 (MQ=255)                                                tCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGc                                                                                       <  1:1128922/140‑1 (MQ=255)                                                  cgcgACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCAt                                                                                     <  1:548178/140‑1 (MQ=255)                                                    cgACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTTTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATcc                                                                                   >  1:348527/1‑140 (MQ=255)                                                    cgACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATcc                                                                                   >  2:871897/1‑140 (MQ=255)                                                     gACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCg                                                                                  >  2:1092319/1‑140 (MQ=255)                                                     gACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCg                                                                                  >  2:148746/1‑140 (MQ=255)                                                     gACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCg                                                                                  >  2:905199/1‑140 (MQ=255)                                                     gACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCg                                                                                  >  1:386429/1‑140 (MQ=255)                                                           cagcagTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTAc                                                                            <  2:1003268/140‑1 (MQ=255)                                                            agcagTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTAcc                                                                           <  2:54716/140‑1 (MQ=255)                                                               agTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTAccgcc                                                                        >  1:1021588/1‑140 (MQ=255)                                                                   cTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCa                                                                    >  1:694044/1‑140 (MQ=255)                                                                    ttCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCaa                                                                   >  1:229661/1‑140 (MQ=255)                                                                      cAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAg                                                                 <  1:357366/140‑1 (MQ=255)                                                                           ttGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCcgc                                                            >  2:184276/1‑140 (MQ=255)                                                                             gcgcAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGa                                                          <  1:871897/140‑1 (MQ=255)                                                                             gcgcAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGa                                                          <  2:229661/140‑1 (MQ=255)                                                                              cgcAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGAt                                                         >  1:164099/1‑140 (MQ=255)                                                                                   caTGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGGCtt                                                    <  2:659141/140‑1 (MQ=255)                                                                                   caTGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACtt                                                    <  2:903217/140‑1 (MQ=255)                                                                                    aTGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACttt                                                   >  2:401017/1‑140 (MQ=255)                                                                                     tGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTg                                                  <  2:763/140‑1 (MQ=255)                                                                                     tGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTg                                                  <  1:1092319/140‑1 (MQ=255)                                                                                     tGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTg                                                  <  1:595549/140‑1 (MQ=255)                                                                                        gATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCg                                               <  1:596301/140‑1 (MQ=255)                                                                                             gTACGCCAGTCTTTTTCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTg                                                  <  1:204652/132‑1 (MQ=255)                                                                                             gTACGCCAGTCTTTTTCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTg                                                  >  2:204652/1‑132 (MQ=255)                                                                                             gTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTa                                          >  1:819590/1‑140 (MQ=255)                                                                                             gTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTa                                          >  1:439407/1‑140 (MQ=255)                                                                                             gTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTa                                          >  1:531369/1‑140 (MQ=255)                                                                                               aCGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACa                                        >  1:416636/1‑140 (MQ=255)                                                                                               aCGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACa                                        <  2:164099/140‑1 (MQ=255)                                                                                                cGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAg                                       >  2:30612/1‑140 (MQ=255)                                                                                                    aGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGAcgg                                   <  2:340475/140‑1 (MQ=255)                                                                                                    aGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGAcgg                                   >  1:536796/1‑140 (MQ=255)                                                                                                      tCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGAcggcg                                 >  1:178164/1‑140 (MQ=255)                                                                                                      tCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGAcggcg                                 >  1:744101/1‑140 (MQ=255)                                                                                                       cTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGAcggcgg                                <  2:532468/140‑1 (MQ=255)                                                                                                        ttttGCCAGCTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGa                               <  1:259739/140‑1 (MQ=255)                                                                                                        ttttGCCAGCTCAACGGCGGCTTCGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGa                               <  1:1019992/140‑1 (MQ=255)                                                                                                                 cTCAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCa                      <  2:313728/140‑1 (MQ=255)                                                                                                                   cAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACg                    <  2:819590/140‑1 (MQ=255)                                                                                                                   cAACGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACg                    <  1:565803/140‑1 (MQ=255)                                                                                                                    aaCGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGt                   <  2:178164/140‑1 (MQ=255)                                                                                                                    aaCGGCGGCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGt                   >  1:41804/1‑140 (MQ=255)                                                                                                                      cggcggCTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCAATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTg                 <  1:150002/140‑1 (MQ=255)                                                                                                                        gcggcTTTGACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGcc               <  2:601821/140‑1 (MQ=255)                                                                                                                                gACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTa       <  2:280601/140‑1 (MQ=255)                                                                                                                                gACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTa       <  1:1045115/140‑1 (MQ=255)                                                                                                                                gACCAGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTa       <  1:480211/140‑1 (MQ=255)                                                                                                                                    aGATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGt   <  2:416636/140‑1 (MQ=255)                                                                                                                                     gATGGTCCTGATGCATTTCCAGTTTATGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTa  <  1:446313/140‑1 (MQ=255)                                                                                                                                     gATGGTCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTa  <  2:536796/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GCTGCACCACGTCACCGTTACCGGTGATGATAAGCGATGCAGTTTCATCCGCGACTGCCAGCAGTGCTTCAAGTTTGCGCAGCATGCGATCGGTACGCCAGTCTTTTGCCAGCTCAACGGCGGCTTTGACCAGATGGCCCTGATGCATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTA  >  NC_000913/4121942‑4122213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑