Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,760	T→C	G1137G (GGT→GGC)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,760	0	T	C	100.0%	238.8 / NA	67	G1137G (GGT→GGC)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/30);  total (37/30)

GATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTA  >  NC_000913/4188629‑4188896                                                                                                                                    |                                                                                                                                         gATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCcgcg                                                                                                                                  <  1:306678/140‑1 (MQ=255) gATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCcgcg                                                                                                                                  <  1:726391/140‑1 (MQ=255)        cAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCg                                                                                                                           <  2:253780/140‑1 (MQ=255)          gcgcAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGa                                                                                                                         >  2:778430/1‑140 (MQ=255)           cgcAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGAc                                                                                                                        <  2:484742/140‑1 (MQ=255)                tACTTCCTGTCCGGTAAAGCGATTGTTCAGCTGCAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCTCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTAGCCTGCCGCGCGGTGCGGACCTGtt                                                                                                                   >  2:767684/1‑140 (MQ=255)                 aCTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTc                                                                                                                  >  1:911765/1‑140 (MQ=255)                         ccGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcg                                                                                                          <  1:873946/140‑1 (MQ=255)                          cGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt                                                                                                         >  2:1085904/1‑140 (MQ=255)                          cGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt                                                                                                         >  2:304470/1‑140 (MQ=255)                          cGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt                                                                                                         >  1:834623/1‑140 (MQ=255)                            ggTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg                                                                                                       <  1:530904/140‑1 (MQ=255)                              tAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc                                                                                                     >  1:776941/1‑140 (MQ=255)                              tAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc                                                                                                     <  2:512601/140‑1 (MQ=255)                                           cAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGc                                                                                        >  2:1061309/1‑140 (MQ=255)                                            aGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGa                                                                                                  <  1:671036/129‑1 (MQ=255)                                            aGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGa                                                                                                  >  2:671036/1‑129 (MQ=255)                                               tGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATc                                                                                    <  2:253576/140‑1 (MQ=255)                                                   aGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTgg                                                                                >  2:1075280/1‑140 (MQ=255)                                                        gCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGaa                                                                           >  1:696579/1‑140 (MQ=255)                                                        gCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGaa                                                                           >  1:996492/1‑140 (MQ=255)                                                           tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATc                                                                        >  2:450496/1‑140 (MQ=255)                                                           tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATc                                                                        >  2:1090464/1‑140 (MQ=255)                                                           tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATc                                                                        >  2:732954/1‑140 (MQ=255)                                                             cAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAg                                                                      <  2:270426/140‑1 (MQ=255)                                                             cAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAg                                                                      <  1:168035/140‑1 (MQ=255)                                                                aTCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCgg                                                                   >  1:627540/1‑140 (MQ=255)                                                                  cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTa                                                                 <  1:919110/140‑1 (MQ=255)                                                                  cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTa                                                                 >  1:1084780/1‑140 (MQ=255)                                                                     ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCg                                                              >  1:557708/1‑140 (MQ=255)                                                                        tGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGttt                                                           >  2:699549/1‑140 (MQ=255)                                                                         ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTc                                                          <  2:996492/140‑1 (MQ=255)                                                                          gTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTcc                                                         >  1:94882/1‑140 (MQ=255)                                                                          gTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTcc                                                         >  1:831620/1‑140 (MQ=255)                                                                          gTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTcc                                                         >  2:72660/1‑140 (MQ=255)                                                                            gACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCtt                                                       <  1:732954/140‑1 (MQ=255)                                                                             acacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTc                                                      <  2:303189/140‑1 (MQ=255)                                                                              cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCg                                                     <  1:735816/140‑1 (MQ=255)                                                                               acCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCgg                                                    >  1:655877/1‑140 (MQ=255)                                                                                    ggCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCATGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               >  2:993032/1‑140 (MQ=255)                                                                                    ggCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               >  1:849685/1‑140 (MQ=255)                                                                                    ggCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               >  2:842695/1‑140 (MQ=255)                                                                                    ggCGCGTATTCCGCAGGAATCCGGCGGTACCAAGAACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               >  1:770555/1‑140 (MQ=255)                                                                                          tATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCa                                         <  1:525683/140‑1 (MQ=255)                                                                                           aTTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCaa                                        >  1:510996/1‑140 (MQ=255)                                                                                                    gAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               <  1:662071/124‑1 (MQ=255)                                                                                                    gAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                               >  2:662071/1‑124 (MQ=255)                                                                                                    gAATCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcg                               <  2:759604/140‑1 (MQ=255)                                                                                                      aTCCGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtc                             >  2:75988/1‑140 (MQ=255)                                                                                                      aTCCGGCGGTACCAAGAACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtc                             <  2:770555/140‑1 (MQ=255)                                                                                                        ccGGCGGTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                           <  1:72660/140‑1 (MQ=255)                                                                                                          ggcggTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCt                         <  1:1075280/140‑1 (MQ=255)                                                                                                          ggcggTACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCt                         <  1:778430/140‑1 (MQ=255)                                                                                                               tACCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTa                    >  1:468073/1‑140 (MQ=255)                                                                                                                aCCAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTAt                   <  1:450496/140‑1 (MQ=255)                                                                                                                  cAAGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCa                 <  2:834623/140‑1 (MQ=255)                                                                                                                    aGGACATCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCAcc               <  2:450261/140‑1 (MQ=255)                                                                                                                         aTCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCAccc              <  1:552415/136‑1 (MQ=255)                                                                                                                         aTCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCAccc              >  2:552415/1‑136 (MQ=255)                                                                                                                         aTCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGt          >  2:36515/1‑140 (MQ=255)                                                                                                                         aTCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGt          >  2:760076/1‑140 (MQ=255)                                                                                                                          tCACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTa         <  2:696579/140‑1 (MQ=255)                                                                                                                           cACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAg        >  2:899641/1‑140 (MQ=255)                                                                                                                           cACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAg        >  2:427581/1‑140 (MQ=255)                                                                                                                           cACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAg        >  1:249755/1‑140 (MQ=255)                                                                                                                           cACCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAg        >  1:87035/1‑140 (MQ=255)                                                                                                                            aCCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCATGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGa       <  1:993032/140‑1 (MQ=255)                                                                                                                            aCCGGTGGCCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGa       <  1:1090464/140‑1 (MQ=255)                                                                                                                                 tggcCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTa  <  1:427581/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTA  >  NC_000913/4188629‑4188896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑