Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,207,132	A→G	V134A (GTG→GCG)	purH ←	bifunctional AICAR transformylase/IMP cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,207,132	0	A	G	100.0%	282.6 / NA	78	V134A (GTG→GCG)	purH	bifunctional AICAR transformylase/IMP cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/34);  total (44/34)

AGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCACCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTCCATAA  >  NC_000913/4206995‑4207267                                                                                                                                          |                                                                                                                                        aGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGcc                                                                                                                                       >  2:276768/1‑140 (MQ=255)    cTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATc                                                                                                                                    >  1:1010876/1‑140 (MQ=255)     tttGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCg                                                                                                                                   >  2:432689/1‑140 (MQ=255)           ggCGAGGTCGAAACGGGTTGCAAGCGTCAGTGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGgc                                                                                                                             >  1:313363/1‑140 (MQ=255)             cGAGGTCGAGACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGgccg                                                                                                                           >  1:117649/1‑140 (MQ=255)             cGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTgg                                                                                                                               >  1:1052581/1‑136 (MQ=255)             cGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTgg                                                                                                                               <  2:1052581/136‑1 (MQ=255)              gAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGgccgc                                                                                                                          <  2:835000/140‑1 (MQ=255)                ggTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGccgccg                                                                                                                        <  2:132468/140‑1 (MQ=255)                ggTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGccgccg                                                                                                                        >  2:887581/1‑140 (MQ=255)                   cGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGata                                                                                                                     >  2:1163647/1‑140 (MQ=255)                      aaCGGGTTGCAAGCGTCAGTGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCg                                                                                                                  <  2:313363/140‑1 (MQ=255)                      aaCGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCg                                                                                                                  <  1:1135517/140‑1 (MQ=255)                      aaCGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCg                                                                                                                  <  1:678593/140‑1 (MQ=255)                      aaCGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCg                                                                                                                  <  2:1010876/140‑1 (MQ=255)                           gTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGtt                                                                                                             >  2:274483/1‑140 (MQ=255)                           gTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGtt                                                                                                             >  2:554786/1‑140 (MQ=255)                            ttGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGTGGCGGAGCGCGCCATCGTAGGGCCGCCGATATCGATGTtc                                                                                                            >  2:1060342/1‑140 (MQ=255)                              gCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTtctc                                                                                                          <  1:1163647/140‑1 (MQ=255)                                   cGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCg                                                                                                     <  1:276768/140‑1 (MQ=255)                                    gTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGc                                                                                                    >  1:185629/1‑140 (MQ=255)                                     tCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCa                                                                                                   >  2:156762/1‑140 (MQ=255)                                     tCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCa                                                                                                   >  1:731594/1‑140 (MQ=255)                                                  gTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGc                                                                                      <  1:694083/140‑1 (MQ=255)                                                      tCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAAcc                                                                                  >  2:399359/1‑140 (MQ=255)                                                      tCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAAcc                                                                                  >  1:141501/1‑140 (MQ=255)                                                          ccatcTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCa                                                                              <  2:812708/140‑1 (MQ=255)                                                          ccatcTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCa                                                                              <  1:99573/140‑1 (MQ=255)                                                                ctTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGcc                                                                        >  2:1119706/1‑140 (MQ=255)                                                                 tttAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGTGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCa                                                                       <  1:1060342/140‑1 (MQ=255)                                                                 tttAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCa                                                                       <  1:1119706/140‑1 (MQ=255)                                                                         tGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTgg                                                               >  1:74749/1‑140 (MQ=255)                                                                         tGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTgg                                                               >  2:480272/1‑140 (MQ=255)                                                                          ggCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTggg                                                              <  1:96528/140‑1 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCa                                                                       >  1:843985/1‑130 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCa                                                                       <  2:843985/130‑1 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTggac                                                             >  1:1036781/1‑138 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  2:959457/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  2:317029/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  2:32480/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  2:408437/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  1:712022/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  1:770647/1‑140 (MQ=255)                                                                           gCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGc                                                             >  1:804100/1‑140 (MQ=255)                                                                                aTAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACg                                                        >  1:819336/1‑140 (MQ=255)                                                                                    tCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATa                                                    <  2:1054647/140‑1 (MQ=255)                                                                                    tCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATa                                                    <  2:819336/140‑1 (MQ=255)                                                                                     cGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATAc                                                   <  1:274483/140‑1 (MQ=255)                                                                                     cGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATAc                                                   >  2:926885/1‑140 (MQ=255)                                                                                      gctgctCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAACAACCTTCACGGGCCACGGTCTGGGCGAACGGATACa                                                  >  2:898775/1‑140 (MQ=255)                                                                                          ctctTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGtt                                              >  1:642682/1‑140 (MQ=255)                                                                                           tctTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTa                                             >  1:889152/1‑140 (MQ=255)                                                                                             ttCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTaac                                           <  2:185629/140‑1 (MQ=255)                                                                                              tCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTaaca                                          >  1:748799/1‑140 (MQ=255)                                                                                               caccacGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAACAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTaacaa                                         <  1:898775/140‑1 (MQ=255)                                                                                                cccacGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTaacaac                                        <  2:74749/139‑1 (MQ=255)                                                                                                accacGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTaacaac                                        <  1:156762/140‑1 (MQ=255)                                                                                                 ccacAATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGAGCCATCGTGGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAacc                                       <  2:613572/140‑1 (MQ=255)                                                                                                    cGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAaccacc                                    <  1:926885/140‑1 (MQ=255)                                                                                                    cGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAaccacc                                    >  2:625265/1‑140 (MQ=255)                                                                                                    cGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAaccacc                                    >  2:415398/1‑140 (MQ=255)                                                                                                     gATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAAccacca                                   <  2:642682/140‑1 (MQ=255)                                                                                                       ttGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCata                                 >  1:978650/1‑140 (MQ=255)                                                                                                          cGACATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATAC‑GGTTAACAACCACCATATCGa                             <  1:432689/140‑1 (MQ=255)                                                                                                            aCATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGAt                            >  1:706882/1‑140 (MQ=255)                                                                                                             cATCTTTATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATa                           <  2:117649/140‑1 (MQ=255)                                                                                                                 tttATGGTTCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCt                       <  1:408437/140‑1 (MQ=255)                                                                                                                         tCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGa               <  1:415398/140‑1 (MQ=255)                                                                                                                         tCTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGa               <  2:712022/140‑1 (MQ=255)                                                                                                                          cTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGGTGGATCTGAt              >  2:716835/1‑140 (MQ=255)                                                                                                                          cTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGAt              >  2:908974/1‑140 (MQ=255)                                                                                                                          cTTGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGAt              >  2:1139772/1‑140 (MQ=255)                                                                                                                           ttGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAg                          >  1:32125/1‑127 (MQ=255)                                                                                                                           ttGGCGGCGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAg                          <  2:32125/127‑1 (MQ=255)                                                                                                                             ggcggcGGAGCGCGCCATCGTTGTGCCGCCGATATCGCTGTTCTCAACCGCATCTTCCAGCTAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGtt           >  2:366034/1‑140 (MQ=255)                                                                                                                             ggcggcGGAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGtt           >  1:376936/1‑140 (MQ=255)                                                                                                                                 gcggAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGttcttc       <  1:480272/140‑1 (MQ=255)                                                                                                                                  cggAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTcc      <  2:731594/140‑1 (MQ=255)                                                                                                                                  cggAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTcc      <  1:399359/140‑1 (MQ=255)                                                                                                                                    gAGCGCGCCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTCCAt    >  1:1358/1‑140 (MQ=255)                                                                                                                                      gcgcgcCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTCCATaa  <  1:554786/140‑1 (MQ=255)                                                                                                                                      gcgcgcCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGACGAACGGATACAGGTTCACAACCACCATATCGATAGGCTGGATCTGATGTTCTTCCATaa  <  2:1036781/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAGCGCACCATCGTTGGGCCGCCGATATCGATGTTCTCAACCGCATCTTCCAGCGAGCAACCTTCACGGGCCACGGTCTGGGCGAACGGATACAGGTTAACAACCACCATATCGATAGGCTGGATCTGATGTTCTTCCATAA  >  NC_000913/4206995‑4207267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑