Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,259,896	A→G	S36S (AGT→AGC)	zur ←	DNA‑binding transcriptional repressor Zur

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,259,896	0	A	G	100.0%	232.2 / NA	64	S36S (AGT→AGC)	zur	DNA‑binding transcriptional repressor Zur
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/33);  total (31/33)

GTGGATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGAA  >  NC_000913/4259759‑4260030                                                                                                                                          |                                                                                                                                       gtggATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCTGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAGATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCt                                                                                                                                      >  2:1086674/1‑140 (MQ=255) gtggATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCt                                                                                                                                      >  1:5757/1‑140 (MQ=255) gtggATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCt                                                                                                                                      >  1:883286/1‑140 (MQ=255)    gATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCtcat                                                                                                                                   >  2:276547/1‑140 (MQ=255)     aTTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCtcatc                                                                                                                                  <  1:146770/140‑1 (MQ=255)      ttCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCtcatca                                                                                                                                 >  1:775696/1‑140 (MQ=255)             ttATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCaa                                                                                                                          <  2:174771/140‑1 (MQ=255)             ttATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCaa                                                                                                                          <  1:1152654/140‑1 (MQ=255)               aTGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAaca                                                                                                                        <  2:866671/140‑1 (MQ=255)               aTGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAaca                                                                                                                        <  1:973811/140‑1 (MQ=255)               aTGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAaca                                                                                                                        <  2:919166/140‑1 (MQ=255)               aTGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAACCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAaca                                                                                                                        <  1:394320/140‑1 (MQ=255)                tGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAacac                                                                                                                       >  2:661634/1‑140 (MQ=255)                tGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAacac                                                                                                                       >  1:831025/1‑140 (MQ=255)                      aaaCCTTGCTCAAGCAGAAAATCCAGCGCGCGATAATCCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAg                                                                                                                 <  2:265647/140‑1 (MQ=255)                      aaaCCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAg                                                                                                                 >  1:843105/1‑140 (MQ=255)                       aaCCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAgg                                                                                                                >  1:889595/1‑140 (MQ=255)                       aaCCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAgg                                                                                                                >  1:154786/1‑140 (MQ=255)                          cTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGgcgc                                                                                                             >  2:870059/1‑140 (MQ=255)                            tGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCtg                                                                                                           >  2:239356/1‑140 (MQ=255)                                 aaGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTgggg                                                                                                      >  2:447061/1‑140 (MQ=255)                                 aaGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTgggg                                                                                                      <  1:234628/140‑1 (MQ=255)                                   gCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTc                                                                                                    >  2:382217/1‑140 (MQ=255)                                   gCACAAAATCCATCGCGTGATAAACCGTTGTCGGCTTGGCTTGCGGTTCAGCTTTGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGTGCAACACGTCCACGCGCTGTGGGGTc                                                                                                    >  2:701686/1‑140 (MQ=255)                                     aGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAg                                                                                                  <  2:240670/140‑1 (MQ=255)                                       aaaaTCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGgc                                                                                                <  2:461249/140‑1 (MQ=255)                                       aaaaTCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGgc                                                                                                <  2:53273/140‑1 (MQ=255)                                                     gATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTgc                                                                                  >  2:728634/1‑140 (MQ=255)                                                               tGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTgcgcg                                                                               >  1:562573/1‑133 (MQ=255)                                                               tGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTgcgcg                                                                               <  2:562573/133‑1 (MQ=255)                                                                      ttggcttgCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGc                                                                 <  2:29941/140‑1 (MQ=255)                                                                      ttggcttgCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGc                                                                 <  1:1067532/140‑1 (MQ=255)                                                                      ttggcttgCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGc                                                                 <  1:870059/140‑1 (MQ=255)                                                                        ggcttgCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCt                                                               <  1:536358/140‑1 (MQ=255)                                                                           ttgCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTgcg                                                            <  2:775696/140‑1 (MQ=255)                                                                             gCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCt                                                          <  2:770078/140‑1 (MQ=255)                                                                              cGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGc                                                                 <  1:361433/132‑1 (MQ=255)                                                                              cGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGc                                                                 >  2:361433/1‑132 (MQ=255)                                                                                  tCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCtaataa                                                     <  1:876317/140‑1 (MQ=255)                                                                                           cgcAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCtaat                                                       <  2:988352/129‑1 (MQ=255)                                                                                           cgcAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCtaat                                                       >  1:988352/1‑129 (MQ=255)                                                                                           cgcAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGt                                            >  2:340339/1‑140 (MQ=255)                                                                                           cgcAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGt                                            >  1:211639/1‑140 (MQ=255)                                                                                                tAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTgg                                       >  1:263341/1‑140 (MQ=255)                                                                                                    tCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCtt                                   <  1:661634/140‑1 (MQ=255)                                                                                                        gCAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTATTAACTCCTGCGTTGTGGTCTTTTcc                               >  2:850423/1‑140 (MQ=255)                                                                                                         cAGATCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCa                              <  1:371999/140‑1 (MQ=255)                                                                                                            aTCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCg                           >  2:245249/1‑140 (MQ=255)                                                                                                             tCATAAGCGCTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCgg                          <  2:646537/140‑1 (MQ=255)                                                                                                                   gcgcTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCt                    <  2:843105/140‑1 (MQ=255)                                                                                                                      cTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCaa                 >  2:647200/1‑140 (MQ=255)                                                                                                                      cTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCaa                 >  2:913140/1‑140 (MQ=255)                                                                                                                      cTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCaa                 >  1:295797/1‑140 (MQ=255)                                                                                                                      cTGATAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCaa                 >  2:306257/1‑140 (MQ=255)                                                                                                                          tAGCGCCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTg             <  1:927579/140‑1 (MQ=255)                                                                                                                             cgcCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCAc                      <  1:307849/128‑1 (MQ=255)                                                                                                                             cgcCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCAc                      >  2:307849/1‑128 (MQ=255)                                                                                                                             cgcCATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTgggg          >  2:183001/1‑140 (MQ=255)                                                                                                                               ccATCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGt        <  1:306257/140‑1 (MQ=255)                                                                                                                                 aTCCTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTa      >  1:54695/1‑140 (MQ=255)                                                                                                                                   ccTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGAATTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAg    >  2:626507/1‑140 (MQ=255)                                                                                                                                    cTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGa   <  1:382217/140‑1 (MQ=255)                                                                                                                                    cTGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGa   <  2:274239/140‑1 (MQ=255)                                                                                                                                     tGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGaa  <  2:5757/140‑1 (MQ=255)                                                                                                                                     tGCAGGCTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGaa  <  2:263341/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GTGGATTCCACCTTATGCACAAAACCTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCAGGCGCACATTACGCTGCGCGCAGATTTTTTCAGCCTGCGCTAATAACTCCTGCGTTGTGGTCTTTTCCATCGGGCACCTCAAAGTGGGGGTTAAGAA  >  NC_000913/4259759‑4260030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑