Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,051	A→G	V193A (GTT→GCT)	melR ←	DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,051	0	A	G	100.0%	257.1 / NA	70	V193A (GTT→GCT)	melR	DNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/28);  total (42/28)

TTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTG  >  NC_000913/4340918‑4341187                                                                                                                                      |                                                                                                                                         tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATaaa                                                                                                                                    >  1:741254/1‑140 (MQ=255) tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATaaa                                                                                                                                    >  1:502692/1‑140 (MQ=255) tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATaaa                                                                                                                                    >  1:392916/1‑140 (MQ=255) tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATaaa                                                                                                                                    >  1:190384/1‑140 (MQ=255) tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATaaa                                                                                                                                    >  1:180837/1‑140 (MQ=255)   tCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAAt                                                                                                                                  >  1:638582/1‑140 (MQ=255)   tCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAAt                                                                                                                                  >  2:670507/1‑140 (MQ=255)     aTCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTg                                                                                                                                <  2:502692/140‑1 (MQ=255)       cGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTgcg                                                                                                                              <  1:545903/140‑1 (MQ=255)           aaTTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATg                                                                                                                          >  1:590309/1‑140 (MQ=255)             ttGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGgc                                                                                                                        >  1:638406/1‑140 (MQ=255)                cATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGcgcg                                                                                                                     <  2:461294/140‑1 (MQ=255)                 aTGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCga                                                                                                                    <  2:590309/140‑1 (MQ=255)                    aCCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGAc                                                                                                                 >  2:359733/1‑140 (MQ=255)                     cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACg                                                                                                                <  2:1115261/140‑1 (MQ=255)                     cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACg                                                                                                                <  1:1077885/140‑1 (MQ=255)                       cGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCt                                                                                                              <  2:638582/140‑1 (MQ=255)                       cGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCt                                                                                                              <  1:359733/140‑1 (MQ=255)                          tGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGtt                                                                                                           >  2:935187/1‑140 (MQ=255)                               tatCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTtgt                                                                                                      >  2:339857/1‑140 (MQ=255)                               tatCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTtgt                                                                                                      >  2:888448/1‑140 (MQ=255)                                  ccccATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACg                                                                                                                >  1:1109228/1‑127 (MQ=255)                                  ccccATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACg                                                                                                                <  2:1109228/127‑1 (MQ=255)                                   cccATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTtgtgtgt                                                                                                  <  2:849943/140‑1 (MQ=255)                                   cccATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTtgtgtgt                                                                                                  >  1:116174/1‑140 (MQ=255)                                      aTTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                                                               <  2:240527/140‑1 (MQ=255)                                      aTTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                                                               <  2:1119809/140‑1 (MQ=255)                                       ttGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                                                              <  2:1080895/140‑1 (MQ=255)                                        tGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGcgcg                                                                                             >  1:659256/1‑140 (MQ=255)                                           aTAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAgg                                                                                          >  2:614109/1‑140 (MQ=255)                                                tGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAgg                                                                                          >  1:103493/1‑135 (MQ=255)                                                tGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAgg                                                                                          <  2:103493/135‑1 (MQ=255)                                                 ggCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                                                              >  1:265728/1‑130 (MQ=255)                                                 ggCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                                                              <  2:265728/130‑1 (MQ=255)                                                  gCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTAtt                                                                                   >  2:571413/1‑140 (MQ=255)                                                  gCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTAtt                                                                                   >  1:394139/1‑140 (MQ=255)                                                    gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTa                                                                                     >  1:1021930/1‑136 (MQ=255)                                                    gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTa                                                                                     <  2:1021930/136‑1 (MQ=255)                                                    gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGa                                                                                 >  1:223048/1‑140 (MQ=255)                                                    gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGa                                                                                 >  1:454476/1‑140 (MQ=255)                                                         gTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGa                                                                            >  2:868498/1‑140 (MQ=255)                                                            tGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATc                                                                         >  1:573882/1‑140 (MQ=255)                                                                   cTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTccc                                                                  >  2:523639/1‑140 (MQ=255)                                                                    tCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCcca                                                                 <  2:741254/140‑1 (MQ=255)                                                                        ccACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCccagcca                                                             >  2:71216/1‑140 (MQ=255)                                                                                 tGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTa                                                    <  1:523639/140‑1 (MQ=255)                                                                                    tGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt                                                 <  1:935187/140‑1 (MQ=255)                                                                                        cAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCt                                             >  1:1155302/1‑140 (MQ=255)                                                                                          gcgcCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTg                                           >  1:292062/1‑140 (MQ=255)                                                                                           cgcCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGa                                          >  2:36157/1‑140 (MQ=255)                                                                                           cgcCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGa                                          >  1:492258/1‑140 (MQ=255)                                                                                               tGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCAt                                      <  1:71216/140‑1 (MQ=255)                                                                                               tGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCAt                                      <  2:573882/140‑1 (MQ=255)                                                                                                gATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATc                                     >  1:575533/1‑140 (MQ=255)                                                                                                 aTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCtt                                            <  2:382779/132‑1 (MQ=255)                                                                                                 aTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCtt                                            >  1:382779/1‑132 (MQ=255)                                                                                                 aTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCa                                    >  2:359403/1‑140 (MQ=255)                                                                                                        ttttCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAgg                                  <  2:84481/135‑1 (MQ=255)                                                                                                        ttttCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAgg                                  >  1:84481/1‑135 (MQ=255)                                                                                                        ttttCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAAt                             >  2:740172/1‑140 (MQ=255)                                                                                                          ttCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAAttt                           >  2:956586/1‑140 (MQ=255)                                                                                                           tCGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtc                          >  1:839095/1‑140 (MQ=255)                                                                                                            cGGCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtca                         >  2:157162/1‑140 (MQ=255)                                                                                                             ggCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtcat                        >  2:754308/1‑140 (MQ=255)                                                                                                              gCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtcatc                       <  2:638406/140‑1 (MQ=255)                                                                                                              gCAATAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtcatc                       <  2:492258/140‑1 (MQ=255)                                                                                                                aaTAAAGCCCAGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCaa                     <  2:223048/140‑1 (MQ=255)                                                                                                                          aGCATCTGGCTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTg           >  1:633379/1‑140 (MQ=255)                                                                                                                                   cTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTg  <  1:157162/140‑1 (MQ=255)                                                                                                                                   cTAGCATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTg  <  1:888448/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTG  >  NC_000913/4340918‑4341187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑