Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,896	A→G	S825S (AGT→AGC)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,896	0	A	G	100.0%	225.2 / NA	62	S825S (AGT→AGC)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/34);  total (28/34)

ACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAG  >  NC_000913/4386761‑4387030                                                                                                                                        |                                                                                                                                       aCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGCAATTGGCTCCAGGCttt                                                                                                                                    >  1:856258/1‑140 (MQ=255)   ccGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCt                                                                                                                                  <  1:441532/140‑1 (MQ=255)      tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACg                                                                                                                               >  2:370314/1‑140 (MQ=255)        cccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGcc                                                                                                                             <  2:856258/140‑1 (MQ=255)           ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTg                                                                                                                          >  1:743059/1‑140 (MQ=255)           ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTg                                                                                                                          >  2:588606/1‑140 (MQ=255)           ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTg                                                                                                                          <  1:599653/140‑1 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTg                                                                                                                          >  1:213144/1‑138 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTg                                                                                                                          <  2:213144/138‑1 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTa                                                                                                                        >  2:904369/1‑140 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTa                                                                                                                        <  1:604638/140‑1 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTa                                                                                                                        <  1:165341/140‑1 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCACCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTa                                                                                                                                 <  2:1001870/131‑1 (MQ=255)             cGTTAAATCCAGGTGCTGCAAAATCACCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTa                                                                                                                                 >  1:1001870/1‑131 (MQ=255)                tAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCg                                                                                                                     <  2:581623/140‑1 (MQ=255)                      cAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAgg                                                                                                               <  1:423831/140‑1 (MQ=255)                         gTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGa                                                                                                            >  1:524530/1‑140 (MQ=255)                            ctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTgg                                                                                                                  <  2:1048668/131‑1 (MQ=255)                            ctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTgg                                                                                                                  >  1:1048668/1‑131 (MQ=255)                              gcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATcc                                                                                                       >  2:731946/1‑140 (MQ=255)                              gcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATcc                                                                                                       <  1:773898/140‑1 (MQ=255)                                aaaaTCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                                                     <  2:979285/140‑1 (MQ=255)                                       ccAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaa                                                                                              >  2:655974/1‑140 (MQ=255)                                                   gcgcGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAgg                                                                                  <  1:615541/140‑1 (MQ=255)                                                    cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa                                                                                 >  1:1145581/1‑140 (MQ=255)                                                     gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                                                >  1:848422/1‑140 (MQ=255)                                                      cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAg                                                                               >  2:170151/1‑140 (MQ=255)                                                             gTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAg                                                                        <  1:731946/140‑1 (MQ=255)                                                                   cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAAt                                                                  >  2:1026995/1‑140 (MQ=255)                                                                    aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATg                                                                 <  1:1068131/140‑1 (MQ=255)                                                                      cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa                                                               <  2:848422/140‑1 (MQ=255)                                                                      cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa                                                               <  2:674577/140‑1 (MQ=255)                                                                      cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa                                                               <  2:510713/140‑1 (MQ=255)                                                                           gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGGCATCCCACAGCGAAATATTTTCCAGGTAGCCGAAAGCGGAATGGATTTCt                                                          <  2:769874/140‑1 (MQ=255)                                                                           gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCt                                                          <  2:1145581/140‑1 (MQ=255)                                                                              tggtgATGATAAACAGGAGAATGGCAATCAGCACCGCACGGAGGGTAATTGGCTGCAGGGTTTCTGCGAACTGTAGCGTGGAGGGGACATCCCACAGCGAAATATTTTGGAGGAAGCGGAAAGCGGAATGGATTTCTGAc                                                       >  2:420936/1‑140 (MQ=255)                                                                                gtgATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAc                                                       <  1:948898/138‑1 (MQ=255)                                                                                gtgATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAc                                                       >  2:948898/1‑138 (MQ=255)                                                                                 tgatgaTAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                                                    <  1:588606/140‑1 (MQ=255)                                                                                     gatAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                                                <  2:166085/140‑1 (MQ=255)                                                                                            acCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGAc                                         <  2:743059/140‑1 (MQ=255)                                                                                             ccAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACa                                        <  1:1026995/140‑1 (MQ=255)                                                                                             ccAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACa                                        >  2:1120312/1‑140 (MQ=255)                                                                                                gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGGATGGATTTCTGACcac                                                    >  1:621541/1‑125 (MQ=255)                                                                                                gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                                                    <  2:621541/125‑1 (MQ=255)                                                                                                gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGaa                                     >  2:48230/1‑140 (MQ=255)                                                                                                 aaTGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGaaa                                    >  1:313072/1‑140 (MQ=255)                                                                                                      cAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACTGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAgg                               >  1:229500/1‑140 (MQ=255)                                                                                                      cAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                                                >  1:646748/1‑123 (MQ=255)                                                                                                      cAATCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                                                <  2:646748/123‑1 (MQ=255)                                                                                                        aTCAGCACCGCACCGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGc                             >  2:623810/1‑140 (MQ=255)                                                                                                            gcaccgcaccGAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATc                         <  1:370314/140‑1 (MQ=255)                                                                                                              accgcaccgAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCaa                       <  1:170151/140‑1 (MQ=255)                                                                                                              accgcaccgAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCaa                       <  2:264302/140‑1 (MQ=255)                                                                                                                cgcaccgAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACa                     >  1:153046/1‑140 (MQ=255)                                                                                                                  caccgAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATc                   >  1:100980/1‑140 (MQ=255)                                                                                                                      gAGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCaaaa               <  2:277908/140‑1 (MQ=255)                                                                                                                       aGGGTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAAt              >  2:144521/1‑140 (MQ=255)                                                                                                                         ggTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTg            <  1:1079096/140‑1 (MQ=255)                                                                                                                          gTAATTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGa           >  2:893490/1‑140 (MQ=255)                                                                                                                            aaTTGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAgc         <  1:893490/140‑1 (MQ=255)                                                                                                                               tGGCTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCa      <  2:100980/140‑1 (MQ=255)                                                                                                                                  cTCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCa   <  2:858171/140‑1 (MQ=255)                                                                                                                                   tCCAGGCTTTCTACGCCCTGTACCGTGGAGGTGATATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAg  >  2:532421/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       ACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAG  >  NC_000913/4386761‑4387030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑