Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,424	G→A	G277D (GGT→GAT)	insD‑6 →	KpLE2 phage‑like element; IS2 insertion element protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,424	0	G	A	100.0%	74.0 / NA	24	G277D (GGT→GAT)	insD‑6	KpLE2 phage‑like element; IS2 insertion element protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTT  >  NC_000913/4499374‑4499555                                                   |                                                                                                                                    tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                            <  1:135262/140‑1 (MQ=11) tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                            >  2:864010/1‑140 (MQ=11)      agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                       <  1:243382/140‑1 (MQ=21)      agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                       <  2:889770/140‑1 (MQ=21)      agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                       <  1:864010/140‑1 (MQ=21)        gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                     >  1:531137/1‑140 (MQ=37)        gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                     >  2:694584/1‑140 (MQ=37)         cGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTg                                    >  2:238585/1‑140 (MQ=37)           ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGat                                  >  2:1165387/1‑140 (MQ=255)              gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATg                               <  1:757283/140‑1 (MQ=255)                     ataACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                        <  2:882284/140‑1 (MQ=255)                     ataACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                        <  1:401071/140‑1 (MQ=255)                     ataACGAATGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                        <  2:260614/140‑1 (MQ=255)                            aTGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtttt                 >  1:286274/1‑140 (MQ=255)                            aTGGCACCCGCATAGTTCGCTGGATCATCGCTCGCCACGCGAATATCTGCCGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtttt                 >  2:86287/1‑140 (MQ=17)                             tGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATg                               <  2:762678/125‑1 (MQ=255)                             tGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATg                               >  1:762678/1‑125 (MQ=255)                             tGGCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTta                >  2:424126/1‑140 (MQ=255)                              ggCATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat               >  2:1001431/1‑140 (MQ=255)                                cATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGaa                             <  2:40264/124‑1 (MQ=255)                                cATCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGaa                             >  1:40264/1‑124 (MQ=255)                                      cATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATa       >  2:1075021/1‑140 (MQ=255)                                           tGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtttt                 <  2:355516/125‑1 (MQ=255)                                           tGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtttt                 >  1:355516/1‑125 (MQ=255)                                           tGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt  <  2:566830/140‑1 (MQ=255)                                                   |                                                                                                                                    TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTT  >  NC_000913/4499374‑4499555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑