Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,528,262	C→T	G219D (GGC→GAC)	sgcQ ←	KpLE2 phage‑like element; putative nucleoside triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,528,262	0	C	T	100.0%	290.3 / NA	81	G219D (GGC→GAC)	sgcQ	KpLE2 phage‑like element; putative nucleoside triphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/41);  total (40/41)

TGCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATGGTTGTTAAATACTGTCGATTTAGC  >  NC_000913/4528127‑4528398                                                                                                                                        |                                                                                                                                         tgCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgt                                                                                                                                      >  2:115373/1‑140 (MQ=255) tgCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgt                                                                                                                                      >  2:427237/1‑140 (MQ=255) tgCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgt                                                                                                                                      >  1:32379/1‑140 (MQ=255)  gCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtg                                                                                                                                     >  2:900374/1‑140 (MQ=255)  gCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtg                                                                                                                                     >  2:719371/1‑140 (MQ=255)  gCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtg                                                                                                                                     >  1:157976/1‑140 (MQ=255)   cACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtgt                                                                                                                                    >  1:1003003/1‑140 (MQ=255)   cACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtgt                                                                                                                                    >  2:1021253/1‑140 (MQ=255)      tttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtgt                                                                                                                                    >  2:285418/1‑137 (MQ=255)      tttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCgtgt                                                                                                                                    <  1:285418/137‑1 (MQ=255)      tttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTgg                                                                                                                                 >  1:1096091/1‑140 (MQ=255)      tttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTgg                                                                                                                                 >  2:856030/1‑140 (MQ=255)      tttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTgg                                                                                                                                 >  1:14463/1‑140 (MQ=255)       ttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGc                                                                                                                                <  2:425389/140‑1 (MQ=255)       ttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGc                                                                                                                                <  2:157976/140‑1 (MQ=255)       ttCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGc                                                                                                                                <  2:355101/140‑1 (MQ=255)        tctcCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGcc                                                                                                                               >  2:662966/1‑140 (MQ=255)        tctcCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGcc                                                                                                                               >  1:435575/1‑140 (MQ=255)        tctcCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCAGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGcc                                                                                                                               >  2:1011717/1‑140 (MQ=255)           ccATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAac                                                                                                                            >  2:472758/1‑140 (MQ=255)               aaaCTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACacga                                                                                                                        <  1:1011717/140‑1 (MQ=255)               aaaCTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACacga                                                                                                                        <  1:472758/140‑1 (MQ=255)               aaaCTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACacga                                                                                                                        <  1:115373/140‑1 (MQ=255)                aaCTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACacgac                                                                                                                       >  1:189192/1‑140 (MQ=255)                 actgactgACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACacgacg                                                                                                                      <  2:1096091/140‑1 (MQ=255)                    gactgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGgtg                                                                                                                   >  1:388166/1‑140 (MQ=255)                    gactgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGgtg                                                                                                                   >  2:208770/1‑140 (MQ=255)                     actgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGgtgt                                                                                                                  <  2:32379/140‑1 (MQ=255)                      ctgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTc                                                                                                                 >  2:962494/1‑140 (MQ=255)                      ctgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTc                                                                                                                 <  1:141826/140‑1 (MQ=255)                      ctgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTc                                                                                                                 >  2:753512/1‑140 (MQ=255)                       tgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCa                                                                                                                >  2:630916/1‑140 (MQ=255)                       tgactCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCa                                                                                                                <  1:900374/140‑1 (MQ=255)                         actCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAgg                                                                                                              <  1:921978/140‑1 (MQ=255)                             gcgcCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCAcc                                                                                                          >  1:918467/1‑140 (MQ=255)                              cgcCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCg                                                                                                         <  2:848442/140‑1 (MQ=255)                                       aaCGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGa                                                                                                <  1:201561/140‑1 (MQ=255)                                       aaCGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGa                                                                                                <  2:752814/140‑1 (MQ=255)                                       aaCGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGa                                                                                                <  2:189192/140‑1 (MQ=255)                                       aaCGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGa                                                                                                <  1:427237/140‑1 (MQ=255)                                         cGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAcc                                                                                              <  1:401125/140‑1 (MQ=255)                                         cGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAcc                                                                                              >  2:460162/1‑140 (MQ=255)                                         cGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAcc                                                                                              >  2:877816/1‑140 (MQ=255)                                                      aaCGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAgcg                                                                                 <  2:575197/140‑1 (MQ=255)                                                        cGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAgcgcg                                                                               <  1:719371/140‑1 (MQ=255)                                                        cGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAgcgcg                                                                               <  2:388166/140‑1 (MQ=255)                                                        cGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGATTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAgcgcg                                                                               >  1:1017968/1‑140 (MQ=255)                                                           cGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAcc                                                                                              >  2:790352/1‑122 (MQ=255)                                                           cGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAcc                                                                                              <  1:790352/122‑1 (MQ=255)                                                                         gtggtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTccc                                                              >  1:925106/1‑140 (MQ=255)                                                                          tggtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcg                                                             <  2:925106/140‑1 (MQ=255)                                                                          tggtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcg                                                             <  2:435575/140‑1 (MQ=255)                                                                           ggtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcgg                                                            >  2:420985/1‑140 (MQ=255)                                                                            gtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcggc                                                           >  2:261012/1‑140 (MQ=255)                                                                            gtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcggc                                                           >  2:573641/1‑140 (MQ=255)                                                                            gtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcggc                                                           >  1:496145/1‑140 (MQ=255)                                                                            gtTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCcggc                                                           >  2:915836/1‑140 (MQ=255)                                                                               gCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGt                                                        <  1:353983/140‑1 (MQ=255)                                                                               gCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGt                                                        <  2:552529/140‑1 (MQ=255)                                                                               gCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGt                                                        <  2:1003003/140‑1 (MQ=255)                                                                                    cacacaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGc                                                   <  2:655566/140‑1 (MQ=255)                                                                                    cacacaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGc                                                   <  1:856030/140‑1 (MQ=255)                                                                                    cacacaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGc                                                   <  1:662966/140‑1 (MQ=255)                                                                                     acacaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGcc                                                  >  2:891777/1‑140 (MQ=255)                                                                                      cacaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCa                                                 <  1:460162/140‑1 (MQ=255)                                                                                       acaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAg                                                >  1:257970/1‑140 (MQ=255)                                                                                       acaGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAg                                                >  1:637850/1‑140 (MQ=255)                                                                                          gCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGaaa                                             >  2:1111006/1‑140 (MQ=255)                                                                                                ggCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACaga                                       <  1:420985/140‑1 (MQ=255)                                                                                                 gCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACagag                                      <  1:753512/140‑1 (MQ=255)                                                                                                 gCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACagag                                      <  2:1017968/140‑1 (MQ=255)                                                                                                 gCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACagag                                      <  2:918467/140‑1 (MQ=255)                                                                                                 gCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACagag                                      <  1:877816/140‑1 (MQ=255)                                                                                                   gATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAgcg                                    >  1:156250/1‑140 (MQ=255)                                                                                                    aTACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAgcgc                                   <  2:14463/140‑1 (MQ=255)                                                                                                     tACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCa                                  >  1:218705/1‑140 (MQ=255)                                                                                                            ttgttCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCTGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGAt                           <  1:630916/140‑1 (MQ=255)                                                                                                            ttgttCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGAt                           <  1:915836/140‑1 (MQ=255)                                                                                                              gttCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATgg                         <  1:648348/140‑1 (MQ=255)                                                                                                               ttCCTCCACGTTTTCCAGGCAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATGgt                        >  1:1096143/1‑140 (MQ=255)                                                                                                                                  cAGACGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATGGTTGTTAAATACTGTCGAttt     >  1:923205/1‑140 (MQ=255)                                                                                                                                     aCGTCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATGGTTGTTAAATACTGTCGATTTAGc  <  2:1155625/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCACTTTCTCCATAAACTGACTGACTCGCGCCTGGTCAACGAAATTGGCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGAGCGCATCAGGATGGTTGTTAAATACTGTCGATTTAGC  >  NC_000913/4528127‑4528398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑