Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,533,630	A→G	L52P (CTA→CCA)	yjhQ ←	KpLE2 phage‑like element; putative acetyltransferase TopAI antitoxin YjhQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,533,630	0	A	G	100.0%	287.0 / NA	79	L52P (CTA→CCA)	yjhQ	KpLE2 phage‑like element; putative acetyltransferase TopAI antitoxin YjhQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/36);  total (43/36)

ACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGAAAAG  >  NC_000913/4533498‑4533765                                                                                                                                     |                                                                                                                                        aCGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGa                                                                                                                                  >  2:898287/1‑140 (MQ=255)       aTCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATAAGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAAcgc                                                                                                                            >  1:1089669/1‑140 (MQ=255)        tCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAAcgcg                                                                                                                           <  2:444483/140‑1 (MQ=255)             tGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCgg                                                                                                                          >  2:936106/1‑136 (MQ=255)             tGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCgg                                                                                                                          <  1:936106/136‑1 (MQ=255)              ggTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGgcgc                                                                                                                     >  2:610277/1‑140 (MQ=255)              ggTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGgcgc                                                                                                                     <  1:1103031/140‑1 (MQ=255)               gTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGgcgcg                                                                                                                    <  1:977977/140‑1 (MQ=255)                   tCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCt                                                                                                                >  1:1115472/1‑140 (MQ=255)                   tCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCt                                                                                                                >  2:887387/1‑140 (MQ=255)                    ccGGTATGACCGCCAGGGGAGCGAGGACGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCtt                                                                                                               >  2:880023/1‑140 (MQ=255)                           gACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt                                                                                                        <  1:541973/140‑1 (MQ=255)                           gACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt                                                                                                        <  2:79512/140‑1 (MQ=255)                            aCCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTc                                                                                                       >  2:849720/1‑140 (MQ=255)                             ccGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCa                                                                                                      <  1:609151/140‑1 (MQ=255)                                ccAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGt                                                                                                   >  2:458846/1‑140 (MQ=255)                                ccAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGt                                                                                                   >  2:540632/1‑140 (MQ=255)                                      gAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAagag                                                                                             >  1:859310/1‑140 (MQ=255)                                      gAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAagag                                                                                             >  1:417222/1‑140 (MQ=255)                                         cGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGcc                                                                                          >  1:297251/1‑140 (MQ=255)                                            ggATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACt                                                                                       <  2:417222/140‑1 (MQ=255)                                               tgtgCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTaaa                                                                                    >  1:1037046/1‑140 (MQ=255)                                                 tgCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATc                                                                                  <  2:398398/140‑1 (MQ=255)                                                  gCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCg                                                                                 >  1:718170/1‑140 (MQ=255)                                                   cATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCgg                                                                                <  2:859310/140‑1 (MQ=255)                                                   cATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCgg                                                                                <  1:292634/140‑1 (MQ=255)                                                   cATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCGTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCgg                                                                                <  2:225519/140‑1 (MQ=255)                                                       aaCGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGcttc                                                                            >  1:183549/1‑140 (MQ=255)                                                        aCGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGcttct                                                                           >  2:451765/1‑140 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        >  2:145907/1‑140 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        >  2:162610/1‑140 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        <  2:909343/140‑1 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        >  1:849378/1‑140 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        >  1:793502/1‑140 (MQ=255)                                                           gTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTg                                                                        >  2:738823/1‑140 (MQ=255)                                                                          cTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATgcg                                                         >  1:878443/1‑140 (MQ=255)                                                                          cTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATgcg                                                         <  2:1022808/140‑1 (MQ=255)                                                                          cTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATgcg                                                         <  2:1031968/140‑1 (MQ=255)                                                                          cTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATgcg                                                         <  2:793502/140‑1 (MQ=255)                                                                          cTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATgcg                                                         >  1:1022808/1‑140 (MQ=255)                                                                                    tGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGa                                               <  2:1068895/140‑1 (MQ=255)                                                                                    tGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGa                                               >  2:738653/1‑140 (MQ=255)                                                                                    tGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGa                                               >  1:1163426/1‑140 (MQ=255)                                                                                     gCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGcgcg                                                       >  2:186367/1‑131 (MQ=255)                                                                                     gCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGcgcg                                                       <  1:186367/131‑1 (MQ=255)                                                                                     gCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGAc                                              >  1:1142182/1‑140 (MQ=255)                                                                                      cccGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACt                                             >  1:639580/1‑140 (MQ=255)                                                                                      cccGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACt                                             <  1:540632/140‑1 (MQ=255)                                                                                      cccGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACt                                             >  1:871054/1‑140 (MQ=255)                                                                                      cccGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACt                                             <  1:898287/140‑1 (MQ=255)                                                                                        cGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTc                                           <  2:661652/140‑1 (MQ=255)                                                                                        cGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTc                                           <  2:297251/140‑1 (MQ=255)                                                                                        cGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTc                                           <  1:458846/140‑1 (MQ=255)                                                                                               ataaaataTGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGata                                    <  2:1163426/140‑1 (MQ=255)                                                                                               ataaaataAGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGata                                    <  2:1089669/140‑1 (MQ=255)                                                                                                 aaaataTGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATc                                  <  2:284922/140‑1 (MQ=255)                                                                                                  aaataTGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCa                                 <  1:451765/140‑1 (MQ=255)                                                                                                     tatGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTg                              >  1:137996/1‑140 (MQ=255)                                                                                                     tatGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTg                              >  2:661959/1‑140 (MQ=255)                                                                                                     tatGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTg                              >  1:638890/1‑140 (MQ=255)                                                                                                     tatGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTg                              >  2:650766/1‑140 (MQ=255)                                                                                                     tatGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTg                              >  2:447203/1‑140 (MQ=255)                                                                                                      atGCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTgg                             <  2:871054/140‑1 (MQ=255)                                                                                                        gCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           <  2:1142182/140‑1 (MQ=255)                                                                                                        gCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           >  2:1121261/1‑140 (MQ=255)                                                                                                        gCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           >  1:581484/1‑140 (MQ=255)                                                                                                        gCCCGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           >  1:481056/1‑140 (MQ=255)                                                                                                         cccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCAt                          <  1:738653/140‑1 (MQ=255)                                                                                                         cccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCAt                          <  1:99343/140‑1 (MQ=255)                                                                                                          ccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           <  2:567236/138‑1 (MQ=255)                                                                                                          ccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCa                           >  1:567236/1‑138 (MQ=255)                                                                                                          ccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATc                         <  2:948073/140‑1 (MQ=255)                                                                                                          ccGACGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATc                         <  2:718170/140‑1 (MQ=255)                                                                                                             aCGGCTTTGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACt                      >  1:1067567/1‑140 (MQ=255)                                                                                                                    tGCCTTCATACCGGGCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCt               <  2:1037046/140‑1 (MQ=255)                                                                                                                                gggCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGaaaa   >  2:1050247/1‑140 (MQ=255)                                                                                                                                 ggCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGAAaag  <  2:849378/140‑1 (MQ=255)                                                                                                                                 ggCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGAAaag  >  2:300484/1‑140 (MQ=255)                                                                                                                                 ggCTGGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGAAaag  >  2:584059/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAAATCGGCTTCTTTGCTGAAGCCAAATGCGCGGGTTTCGACTTCCCGGATATCACTGGCATCACTTTTATCTGTGATATGAAAAG  >  NC_000913/4533498‑4533765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑