Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,588,280	A→G	F196L (TTC→CTC)	yjiA ←	P‑loop guanosine triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,588,280	0	A	G	100.0%	171.5 / NA	49	F196L (TTC→CTC)	yjiA	P‑loop guanosine triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/23);  total (26/23)

CGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTCAGCAGAATGCGGTCGGC  >  NC_000913/4588141‑4588412                                                                                                                                            |                                                                                                                                     cgGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAAC‑‑‑GTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGcag                                                                                                                                   <  2:982771/140‑1 (MQ=255)    gAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGcag                                                                                                                                   >  1:842251/1‑140 (MQ=255)     aaaCTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGagcagc                                                                                                                                  <  2:210507/140‑1 (MQ=255)           cGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGcag                                                                                                                                   >  2:1145359/1‑133 (MQ=255)           cGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGcag                                                                                                                                   <  1:1145359/133‑1 (MQ=255)                   tCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGAt                                                                                                                    <  1:255435/140‑1 (MQ=255)                       ccGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCg                                                                                                                >  2:934282/1‑140 (MQ=255)                          ggTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCg                                                                                                             >  2:737204/1‑140 (MQ=255)                            tAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTg                                                                                                           >  2:1054562/1‑140 (MQ=255)                            tAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTg                                                                                                           >  1:93572/1‑140 (MQ=255)                              aTCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTggg                                                                                                         <  1:1054562/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  1:207478/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  1:934282/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  2:132639/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  1:749718/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  1:623539/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  2:842251/140‑1 (MQ=255)                                 cAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      >  2:951541/1‑140 (MQ=255)                                    ttCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCg                                                                                                                >  2:648261/1‑127 (MQ=255)                                    ttCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCg                                                                                                                <  1:648261/127‑1 (MQ=255)                                    ttCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  2:409620/137‑1 (MQ=255)                                    ttCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      >  1:409620/1‑137 (MQ=255)                                         ccacAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      >  2:1078191/1‑132 (MQ=255)                                         ccacAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGa                                                                                                      <  1:1078191/132‑1 (MQ=255)                                           acaATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGAc                                                                                            <  2:1155253/140‑1 (MQ=255)                                             aaTCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGAc                                                                                            <  2:252641/138‑1 (MQ=255)                                             aaTCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGAc                                                                                            >  1:252641/1‑138 (MQ=255)                                              aTCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCgg                                                                                         >  2:695689/1‑140 (MQ=255)                                                     aaaTATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCgg                                                                                  <  1:695689/140‑1 (MQ=255)                                                                       cTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAgg                                                                >  2:1160369/1‑140 (MQ=255)                                                                          cGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGt                                                             <  1:1160369/140‑1 (MQ=255)                                                                          cGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGt                                                             >  1:621244/1‑140 (MQ=255)                                                                          cGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGt                                                             >  2:644572/1‑140 (MQ=255)                                                                          cGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGt                                                             >  1:724304/1‑140 (MQ=255)                                                                            aTAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTc                                                           >  2:176623/1‑140 (MQ=255)                                                                             tAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCa                                                          <  1:951541/140‑1 (MQ=255)                                                                                         gcgGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCt                                              >  2:672112/1‑140 (MQ=255)                                                                                            gTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCt                                           >  2:308733/1‑140 (MQ=255)                                                                                            gTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCt                                           >  1:778662/1‑140 (MQ=255)                                                                                                         gacgTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGt                              <  2:93572/140‑1 (MQ=255)                                                                                                           cgTTTTCTTCCAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCg                            >  2:580073/1‑140 (MQ=255)                                                                                                                     cAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTca                  >  1:1090794/1‑140 (MQ=255)                                                                                                                     cAGCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTca                  >  2:17893/1‑140 (MQ=255)                                                                                                                       gCATAAAACCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTcagc                >  2:919027/1‑140 (MQ=255)                                                                                                                           aaaaCCGTTGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTCAGCAGaa            <  2:527086/140‑1 (MQ=255)                                                                                                                                  ttGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTgg                     <  1:360511/124‑1 (MQ=255)                                                                                                                                  ttGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTgg                     >  2:360511/1‑124 (MQ=255)                                                                                                                                  ttGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGt                    <  1:306003/125‑1 (MQ=255)                                                                                                                                  ttGGTGTTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGt                    >  2:306003/1‑125 (MQ=255)                                                                                                                                     gtgtTGAGCAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTCAGCAGAATGCGGTCGGc  <  1:875456/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     CGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGACGGTGTAGACCGGTGCGCGGGCGTTGATGCGCGCCAGGCGTTCATGCAGTTTTTCTGCTTCGCCTGCGACGTCGGTTTTGGTCAGCAGAATGCGGTCGGC  >  NC_000913/4588141‑4588412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑