Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,494	A→G	Y596H (TAC→CAC)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,494	0	A	G	100.0%	296.9 / NA	82	Y596H (TAC→CAC)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/42);  total (40/42)

CTCTTTGTACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTATTGGGTGCGCTTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAG  >  NC_000913/4589366‑4589629                                                                                                                                 |                                                                                                                                        ctctTTGTACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTgcgcc                                                                                                                              <  2:1104593/140‑2 (MQ=255) ctctTTGTACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTgcgcc                                                                                                                              <  2:697124/140‑2 (MQ=255)        tACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCtt                                                                                                                       >  1:1135738/1‑140 (MQ=255)           tGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAAt                                                                                                                    <  2:672993/140‑1 (MQ=255)             gCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCa                                                                                                                  >  2:870787/1‑140 (MQ=255)              cTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAg                                                                                                                 >  2:171131/1‑140 (MQ=255)              cTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCACCTTAATCAg                                                                                                                 >  2:462407/1‑140 (MQ=255)                tGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGca                                                                                                               >  1:14281/1‑140 (MQ=255)                tGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGca                                                                                                               >  1:856873/1‑140 (MQ=255)                  ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTgcgcc                                                                                                                              >  2:867146/1‑122 (MQ=255)                  ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTgcgcc                                                                                                                              <  1:867146/123‑2 (MQ=255)                  ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGcaca                                                                                                             >  2:684432/1‑140 (MQ=255)                  ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGcaca                                                                                                             >  1:1097944/1‑140 (MQ=255)                   cATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACaa                                                                                                            <  2:1097944/140‑1 (MQ=255)                      gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGTAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                         <  2:349447/140‑1 (MQ=255)                      gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                         <  2:1135738/140‑1 (MQ=255)                      gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                         <  2:856873/140‑1 (MQ=255)                       tAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGt                                                                                                        >  2:572409/1‑140 (MQ=255)                       tAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGt                                                                                                        >  1:1027317/1‑140 (MQ=255)                        agaagaAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTg                                                                                                       <  2:527955/140‑1 (MQ=255)                         gaagaaGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGc                                                                                                      <  2:665852/140‑1 (MQ=255)                          aagaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGcc                                                                                                     >  1:296496/1‑140 (MQ=255)                            gaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                         >  2:1153534/1‑134 (MQ=255)                            gaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                         <  1:1153534/134‑1 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGc                                                                                                                >  2:107634/1‑124 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGc                                                                                                                <  1:107634/124‑1 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                >  2:17180/1‑140 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                >  2:271865/1‑140 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                >  1:69819/1‑140 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                >  1:1060686/1‑140 (MQ=255)                               gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                >  2:737729/1‑140 (MQ=255)                                ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                               <  1:715685/140‑1 (MQ=255)                                ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                               >  1:66973/1‑140 (MQ=255)                                ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                               >  1:690309/1‑140 (MQ=255)                                             ggTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCt                                                                                  >  1:689360/1‑140 (MQ=255)                                             ggTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCt                                                                                  >  2:599877/1‑140 (MQ=255)                                               ttGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGc                                                                                <  2:1060968/140‑1 (MQ=255)                                                tGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGcc                                                                               <  1:271865/140‑1 (MQ=255)                                                tGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGcc                                                                               <  2:66973/140‑1 (MQ=255)                                                     cTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                          <  1:737729/140‑1 (MQ=255)                                                     cTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                          <  1:398961/140‑1 (MQ=255)                                                      tGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATc                                                                         <  1:313975/140‑1 (MQ=255)                                                          cAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                     <  1:508260/140‑1 (MQ=255)                                                             tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTgg                                                                  <  2:1060686/140‑1 (MQ=255)                                                             tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGAGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCa                                                                           <  1:162177/131‑1 (MQ=255)                                                             tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGAGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCa                                                                           >  2:162177/1‑131 (MQ=255)                                                               tCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGTAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                          >  2:800880/1‑130 (MQ=255)                                                               tCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                          <  1:800880/130‑1 (MQ=255)                                                                  ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                     <  2:1159550/132‑1 (MQ=255)                                                                  ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                     >  1:1159550/1‑132 (MQ=255)                                                                  ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATa                                                             >  1:706036/1‑140 (MQ=255)                                                                      cAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGa                                                         <  2:69819/140‑1 (MQ=255)                                                                       aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                        >  1:50742/1‑140 (MQ=255)                                                                       aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                        <  1:17180/140‑1 (MQ=255)                                                                       aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                        <  1:171131/140‑1 (MQ=255)                                                                       aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCACCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                        <  1:462407/140‑1 (MQ=255)                                                                        gcgcCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAAc                                                       >  1:1112428/1‑140 (MQ=255)                                                                         cgcCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACa                                                      <  1:599877/140‑1 (MQ=255)                                                                            ccAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCg                                                   <  1:684432/140‑1 (MQ=255)                                                                             cAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCgg                                                  >  1:911379/1‑140 (MQ=255)                                                                                gtggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCca                                               <  2:690309/140‑1 (MQ=255)                                                                                gtggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCca                                               <  1:641779/140‑1 (MQ=255)                                                                                 tggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCcac                                              <  1:870787/140‑1 (MQ=255)                                                                                 tggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCcac                                              <  2:804032/140‑1 (MQ=255)                                                                                   gtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAg                                            <  2:50742/140‑1 (MQ=255)                                                                                      cAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGct                                         >  1:126175/1‑140 (MQ=255)                                                                                              cagcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgc                                 >  2:504051/1‑140 (MQ=255)                                                                                              cagcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgc                                 >  1:527687/1‑140 (MQ=255)                                                                                                gcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgccg                               <  2:963065/140‑1 (MQ=255)                                                                                                   aTACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGccc                            >  1:598923/1‑140 (MQ=255)                                                                                                      cAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGc                         <  1:443761/140‑1 (MQ=255)                                                                                                         ccGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGa                      >  1:694159/1‑140 (MQ=255)                                                                                                              acaacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAAc                 >  2:752333/1‑140 (MQ=255)                                                                                                              acaacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAAc                 <  2:527687/140‑1 (MQ=255)                                                                                                                aacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCa               <  2:694159/140‑1 (MQ=255)                                                                                                                aacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCa               <  2:1027317/140‑1 (MQ=255)                                                                                                                  caGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACg             >  1:457777/1‑140 (MQ=255)                                                                                                                   aGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGc            <  1:752333/140‑1 (MQ=255)                                                                                                                       aCCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTg        >  1:520462/1‑140 (MQ=255)                                                                                                                        cccAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGa       >  2:71501/1‑140 (MQ=255)                                                                                                                            gATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAca   <  2:706036/140‑1 (MQ=255)                                                                                                                             aTGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAcag  >  2:885217/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        CTCTTTGTACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTATTGGGTGCGCTTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAG  >  NC_000913/4589366‑4589629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑