Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,505	T→C	K592R (AAG→AGG)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,505	0	T	C	100.0%	316.9 / NA	87	K592R (AAG→AGG)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/44);  total (43/44)

TACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTATTGGGTGCGCTTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCA  >  NC_000913/4589373‑4589641                                                                                                                                     |                                                                                                                                         tACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCtt                                                                                                                                   >  1:1135738/1‑140 (MQ=255)    tGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAAt                                                                                                                                <  2:672993/140‑1 (MQ=255)      gCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCa                                                                                                                              >  2:870787/1‑140 (MQ=255)       cTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAg                                                                                                                             >  2:171131/1‑140 (MQ=255)       cTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCACCTTAATCAg                                                                                                                             >  2:462407/1‑140 (MQ=255)         tGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGca                                                                                                                           >  1:856873/1‑140 (MQ=255)         tGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGca                                                                                                                           >  1:14281/1‑140 (MQ=255)           ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGcaca                                                                                                                         >  1:1097944/1‑140 (MQ=255)           ccATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGcaca                                                                                                                         >  2:684432/1‑140 (MQ=255)            cATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACaa                                                                                                                        <  2:1097944/140‑1 (MQ=255)               gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGTAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                                     <  2:349447/140‑1 (MQ=255)               gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                                     <  2:1135738/140‑1 (MQ=255)               gTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                                     <  2:856873/140‑1 (MQ=255)                tAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGt                                                                                                                    >  1:1027317/1‑140 (MQ=255)                tAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGt                                                                                                                    >  2:572409/1‑140 (MQ=255)                 agaagaAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTg                                                                                                                   <  2:527955/140‑1 (MQ=255)                  gaagaaGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGc                                                                                                                  <  2:665852/140‑1 (MQ=255)                   aagaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGcc                                                                                                                 >  1:296496/1‑140 (MQ=255)                     gaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                                     >  2:1153534/1‑134 (MQ=255)                     gaagCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACgg                                                                                                                     <  1:1153534/134‑1 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGc                                                                                                                            <  1:107634/124‑1 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGc                                                                                                                            >  2:107634/1‑124 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                            >  2:271865/1‑140 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                            >  2:17180/1‑140 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                            >  2:737729/1‑140 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                            >  1:1060686/1‑140 (MQ=255)                        gCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTa                                                                                                            >  1:69819/1‑140 (MQ=255)                         ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                                           <  1:715685/140‑1 (MQ=255)                         ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                                           >  1:690309/1‑140 (MQ=255)                         ccTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTAc                                                                                                           >  1:66973/1‑140 (MQ=255)                                      ggTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCt                                                                                              >  2:599877/1‑140 (MQ=255)                                      ggTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCt                                                                                              >  1:689360/1‑140 (MQ=255)                                        ttGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGc                                                                                            <  2:1060968/140‑1 (MQ=255)                                         tGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGcc                                                                                           <  1:271865/140‑1 (MQ=255)                                         tGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGcc                                                                                           <  2:66973/140‑1 (MQ=255)                                              cTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                                      <  1:398961/140‑1 (MQ=255)                                              cTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                                      <  1:737729/140‑1 (MQ=255)                                               tGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATc                                                                                     <  1:313975/140‑1 (MQ=255)                                                   cAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                                 <  1:508260/140‑1 (MQ=255)                                                      tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTgg                                                                              <  2:1060686/140‑1 (MQ=255)                                                      tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGAGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCa                                                                                       >  2:162177/1‑131 (MQ=255)                                                      tttCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGAGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCa                                                                                       <  1:162177/131‑1 (MQ=255)                                                        tCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGTAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                                      >  2:800880/1‑130 (MQ=255)                                                        tCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCAt                                                                                      <  1:800880/130‑1 (MQ=255)                                                           ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                                 <  2:1159550/132‑1 (MQ=255)                                                           ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGt                                                                                 >  1:1159550/1‑132 (MQ=255)                                                           ggCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATa                                                                         >  1:706036/1‑140 (MQ=255)                                                               cAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGa                                                                     <  2:69819/140‑1 (MQ=255)                                                                aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                                    >  1:50742/1‑140 (MQ=255)                                                                aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                                    <  1:171131/140‑1 (MQ=255)                                                                aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                                    <  1:17180/140‑1 (MQ=255)                                                                aGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCACCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGaa                                                                    <  1:462407/140‑1 (MQ=255)                                                                 gcgcCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAAc                                                                   >  1:1112428/1‑140 (MQ=255)                                                                  cgcCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACa                                                                  <  1:599877/140‑1 (MQ=255)                                                                     ccAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCg                                                               <  1:684432/140‑1 (MQ=255)                                                                      cAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCgg                                                              >  1:911379/1‑140 (MQ=255)                                                                         gtggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCca                                                           <  2:690309/140‑1 (MQ=255)                                                                         gtggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCca                                                           <  1:641779/140‑1 (MQ=255)                                                                          tggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCcac                                                          <  2:804032/140‑1 (MQ=255)                                                                          tggtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCcac                                                          <  1:870787/140‑1 (MQ=255)                                                                            gtgCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAg                                                        <  2:50742/140‑1 (MQ=255)                                                                               cAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGct                                                     >  1:126175/1‑140 (MQ=255)                                                                                       cagcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgc                                             >  1:527687/1‑140 (MQ=255)                                                                                       cagcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgc                                             >  2:504051/1‑140 (MQ=255)                                                                                         gcCATACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAAcgccg                                           <  2:963065/140‑1 (MQ=255)                                                                                            aTACAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGccc                                        >  1:598923/1‑140 (MQ=255)                                                                                               cAGCCGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGc                                     <  1:443761/140‑1 (MQ=255)                                                                                                  ccGGAACAACAGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGa                                  >  1:694159/1‑140 (MQ=255)                                                                                                       acaacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAAc                             <  2:527687/140‑1 (MQ=255)                                                                                                       acaacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAAc                             >  2:752333/1‑140 (MQ=255)                                                                                                         aacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCa                           <  2:1027317/140‑1 (MQ=255)                                                                                                         aacaGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCa                           <  2:694159/140‑1 (MQ=255)                                                                                                           caGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACg                         >  1:457777/1‑140 (MQ=255)                                                                                                            aGTTACCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGc                        <  1:752333/140‑1 (MQ=255)                                                                                                                aCCCAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTg                    >  1:520462/1‑140 (MQ=255)                                                                                                                 cccAGATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGa                   >  2:71501/1‑140 (MQ=255)                                                                                                                     gATGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAca               <  2:706036/140‑1 (MQ=255)                                                                                                                      aTGTGTTGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAcag              >  2:885217/1‑140 (MQ=255)                                                                                                                        gtgttGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAcagca            >  1:564725/4‑140 (MQ=255)                                                                                                                         tgttGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATAcagcag           >  2:386079/3‑140 (MQ=255)                                                                                                                           ttGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTg                    <  2:229242/129‑1 (MQ=255)                                                                                                                           ttGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTg                    >  1:229242/1‑129 (MQ=255)                                                                                                                           ttGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGt         >  1:1154664/1‑140 (MQ=255)                                                                                                                            tGGGTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTa        <  2:564725/140‑1 (MQ=255)                                                                                                                             gggTGCGCCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAg       <  1:572409/140‑1 (MQ=255)                                                                                                                                  cgcCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTg                    <  2:57032/122‑1 (MQ=255)                                                                                                                                  cgcCTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCca  <  1:504051/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TACTGGCTTGCCATGTAGAAGAAGCCTTCCATCTGCGGGTTGGTGCTGAACAGTTTCAGGCCCAGCGCCCAGGTGGTGCAGATAAGCAGCCATACAGCCGGAACAACAGTTACCCAGATGTATTGGGTGCGCTTCATCTTAATCAGCACAACGGTGCCCAGTACCAGCGCTACGGCTGCCAGCATCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCA  >  NC_000913/4589373‑4589641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑