Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	546,079	T→C	E95E (GAA→GAG)	allD ←	ureidoglycolate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	546,079	0	T	C	100.0%	163.7 / NA	46	E95E (GAA→GAG)	allD	ureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

CGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTAT  >  NC_000913/545943‑546214                                                                                                                                         |                                                                                                                                        cGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTcc                                                                                                                                      <  1:408468/140‑1 (MQ=255)        gCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCa                                                                                                                               >  2:447520/1‑140 (MQ=255)                      cAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGAcc                                                                                                                 >  1:223602/1‑140 (MQ=255)                        aTGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCACAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTg                                                                                                               <  2:1040592/140‑1 (MQ=255)                            aTCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCcg                                                                                                           <  2:248318/140‑1 (MQ=255)                             tCCGGCGCGGGCTGCCTGCTGCACAAAATAAGGGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCcgg                                                                                                          >  2:1064230/1‑140 (MQ=255)                             tCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCcgg                                                                                                          >  1:907707/1‑140 (MQ=255)                               cGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCcggcg                                                                                                        <  2:223602/140‑1 (MQ=255)                                ggCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCcggcgg                                                                                                       <  2:209551/140‑1 (MQ=255)                                  cgcgGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCa                                                                                                     >  2:724578/1‑140 (MQ=255)                                   gcgGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCAt                                                                                                    <  1:447520/140‑1 (MQ=255)                                        ctgcctgcTGCACAAAATAAGGGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCg                                                                                               <  1:1064230/140‑1 (MQ=255)                                                 gcACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTaaa                                                                                      >  2:405265/1‑140 (MQ=255)                                                  cacaAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTaaaa                                                                                     <  2:907707/140‑1 (MQ=255)                                                  cacaAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTaaaa                                                                                     <  1:724578/140‑1 (MQ=255)                                                     aaaaTAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTg                                                                                  >  1:773782/1‑140 (MQ=255)                                                      aaaTAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGc                                                                                 <  2:448999/140‑1 (MQ=255)                                                                ttGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGc                                                                       <  1:146454/140‑1 (MQ=255)                                                                 tGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGcc                                                                      <  2:315263/140‑1 (MQ=255)                                                                 tGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGcc                                                                      >  1:1010088/1‑140 (MQ=255)                                                                 tGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGcc                                                                      >  2:972902/1‑140 (MQ=255)                                                                 tGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGcc                                                                      >  1:682697/1‑140 (MQ=255)                                                                        cTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTc                                                               >  1:650043/1‑140 (MQ=255)                                                                                  tCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGa                                                     >  1:632808/1‑140 (MQ=255)                                                                                       cTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTc                                                >  2:707765/1‑140 (MQ=255)                                                                                            aCCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGtt                                           <  1:405265/140‑1 (MQ=255)                                                                                              cGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTcg                                         <  2:650043/140‑1 (MQ=255)                                                                                                accaccGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTcgcg                                       >  1:659089/1‑140 (MQ=255)                                                                                                accaccGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTcgcg                                       >  1:57810/1‑140 (MQ=255)                                                                                                accaccGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTcgcg                                       >  2:572515/1‑140 (MQ=255)                                                                                                     cGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTgg                                  >  1:955886/1‑140 (MQ=255)                                                                                                     cGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTgg                                  >  2:407039/1‑140 (MQ=255)                                                                                                         aCGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTgcc                              <  2:659089/140‑1 (MQ=255)                                                                                                         aCGCCATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTgcc                              <  1:466638/140‑1 (MQ=255)                                                                                                            ccATTTTGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTgccgcc                           >  1:577390/1‑140 (MQ=255)                                                                                                               ttttGCTGGGCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCttt                        >  2:1007746/1‑140 (MQ=255)                                                                                                                       ggCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGc                >  2:814282/1‑140 (MQ=255)                                                                                                                       ggCGGTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGc                >  1:192299/1‑140 (MQ=255)                                                                                                                          ggTTTTGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGtt             <  2:773782/140‑1 (MQ=255)                                                                                                                             tttGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcg          <  1:972902/140‑1 (MQ=255)                                                                                                                             tttGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcg          <  1:308443/140‑1 (MQ=255)                                                                                                                               tGATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgcg        <  1:707765/140‑1 (MQ=255)                                                                                                                                gATGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgt       <  2:632808/140‑1 (MQ=255)                                                                                                                                 aTGGCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgta      >  1:586894/1‑140 (MQ=255)                                                                                                                                   ggCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAAt                  >  2:84217/1‑126 (MQ=255)                                                                                                                                   ggCATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAAt                  <  1:84217/126‑1 (MQ=255)                                                                                                                                     cATGCTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTAt  <  1:814282/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTAT  >  NC_000913/545943‑546214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑