Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,603	T→C	L85L (TTG→CTG)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,603	0	T	C	100.0%	176.8 / NA	49	L85L (TTG→CTG)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/31);  total (18/31)

GGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCT  >  NC_000913/71467‑71724                                                                                                                                         |                                                                                                                          ggTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGc                                                                                                                        <  2:468987/140‑1 (MQ=255)   tGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGGTTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGGTGGCGTTTTAAAAAGCCGCTGCAt                                                                                                                      >  1:3455/1‑140 (MQ=255)   tGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCAt                                                                                                                      <  2:390320/140‑1 (MQ=255)   tGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCAt                                                                                                                      <  2:591472/140‑1 (MQ=255)   tGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCAt                                                                                                                      >  2:529922/1‑140 (MQ=255)     cTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCg                                                                                                                    <  1:134648/140‑1 (MQ=255)     cTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCg                                                                                                                    <  1:135552/140‑1 (MQ=255)     cTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCg                                                                                                                    <  2:330201/140‑1 (MQ=255)        aTGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTg                                                                                                                 >  1:114584/1‑140 (MQ=255)        aTGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTTTGGCACGCCCGGCTGGGAGGGGTTATTGGCTGCTTGATGGGCGGCTGGGTTTTTTTCTTGCTGGGGTGGCGTTTTAAAAAACCGCTGCCTCGCTg                                                                                                                 >  2:569750/1‑140 (MQ=255)        aTGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGGTGGCGTTTTAAAAAGCCGCTGCATCGCTg                                                                                                                 >  1:589681/1‑140 (MQ=255)                gCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTc                                                                                                         <  1:625871/140‑1 (MQ=255)                gCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTc                                                                                                         <  2:796553/140‑1 (MQ=255)                  tGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTg                                                                                                       <  1:569750/140‑1 (MQ=255)                       gcgcTGATTGGCAGCGGTGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTgaagaa                                                                                                  >  2:127296/1‑140 (MQ=255)                         gcTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGaaaa                                                                                                >  1:90425/1‑140 (MQ=255)                          cTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGTTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGaaaaa                                                                                               >  2:109798/1‑140 (MQ=255)                            gATTGGCAGCGGTGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACa                                                                                             <  1:127296/140‑1 (MQ=255)                             aTTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACaa                                                                                            >  1:564917/1‑140 (MQ=255)                             aTTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACaa                                                                                            <  2:564917/140‑1 (MQ=255)                               tGGCAGCGGCGAGTTAAGTTTCTGGCACACCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAg                                                                                          <  1:890024/140‑1 (MQ=255)                                 gCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCa                                                                                        <  2:460073/140‑1 (MQ=255)                                 gCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCa                                                                                        <  1:85333/140‑1 (MQ=255)                                    gcggcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGTTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCacta                                                                                     <  1:109798/140‑1 (MQ=255)                                    gcggcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCacta                                                                                     <  2:18255/140‑1 (MQ=255)                                       gcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACtt                                                                                  >  2:1091708/1‑140 (MQ=255)                                       gcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACtt                                                                                  >  1:764383/1‑140 (MQ=255)                                          aGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGAt                                                                               <  2:90425/140‑1 (MQ=255)                                           gTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGTCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATa                                                                              <  2:553693/140‑1 (MQ=255)                                                    cTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAAc                                                                     <  2:114584/140‑1 (MQ=255)                                                    cTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAAc                                                                     >  1:223675/1‑140 (MQ=255)                                                        cACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGc                                                                 <  1:753220/140‑1 (MQ=255)                                                              tggctggcAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCa                                                           <  1:848720/140‑1 (MQ=255)                                                               ggctggcAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCAt                                                          <  1:692468/140‑1 (MQ=255)                                                               ggctggcAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCAt                                                          <  1:4289/140‑1 (MQ=255)                                                               ggctggcAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCAt                                                          <  1:172001/140‑1 (MQ=255)                                                                     cAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAacac                                                    <  1:689514/140‑1 (MQ=255)                                                                      aGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAAcaca                                                   <  1:529922/140‑1 (MQ=255)                                                                      aGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAAcaca                                                   <  2:223675/140‑1 (MQ=255)                                                                          attattGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCAt                                               >  1:976031/1‑140 (MQ=255)                                                                          attattGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCAt                                               >  1:453196/1‑140 (MQ=255)                                                                              ttGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTc                                           >  1:1090862/1‑140 (MQ=255)                                                                               tGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCa                                          <  2:1090862/140‑1 (MQ=255)                                                                                  ctgctTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCa                                          >  2:247588/1‑137 (MQ=255)                                                                                  ctgctTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCa                                          <  1:247588/137‑1 (MQ=255)                                                                                   tgctTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCAt                                      >  1:997030/1‑140 (MQ=255)                                                                                    gctTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCAtt                                     >  2:992533/1‑140 (MQ=255)                                                                                               gACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGgtcggtcg                          >  1:208045/1‑140 (MQ=255)                                                                                                aCTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGt                         >  2:65819/1‑140 (MQ=255)                                                                                                                   gggTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTc      >  1:1044398/1‑140 (MQ=255)                                                                                                                     gTTGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCg    <  1:544232/140‑1 (MQ=255)                                                                                                                       tGGCGTTTTAAAAAGCCGCTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCt  <  2:997030/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                          GGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCT  >  NC_000913/71467‑71724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑