Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	993,892	A→G	S51S (AGT→AGC)	ssuB ←	aliphatic sulfonate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	993,892	0	A	G	100.0%	73.1 / NA	22	S51S (AGT→AGC)	ssuB	aliphatic sulfonate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/6);  total (16/6)

ACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCT  >  NC_000913/993755‑994022                                                                                                                                          |                                                                                                                                   aCCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCt                                                                                                                                  >  1:856494/1‑140 (MQ=255)    gATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGCCCTGCCAGCAGGCGCAGCAGGGTGCtttt                                                                                                                               <  2:530184/140‑1 (MQ=255)      tttCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGc                                                                                                                             <  2:816534/140‑1 (MQ=255)      tttCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGc                                                                                                                             >  1:273658/1‑140 (MQ=255)         ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGccac                                                                                                                          <  1:781122/140‑1 (MQ=255)          cACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGccacc                                                                                                                         <  1:530836/140‑1 (MQ=255)               tAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGc                                                                                                                    <  1:214890/140‑1 (MQ=255)                 gCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTg                                                                                                                  <  2:856494/140‑1 (MQ=255)                  cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGc                                                                                                                 >  1:155319/1‑140 (MQ=255)                       gcgcATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGccc                                                                                                            >  2:290074/1‑140 (MQ=255)                                       catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACt                                                                                            >  1:1059706/1‑140 (MQ=255)                                       catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACt                                                                                            >  1:794547/1‑140 (MQ=255)                                       catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACt                                                                                            >  2:409325/1‑140 (MQ=255)                                         ttCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGa                                                                                          >  1:491501/1‑140 (MQ=255)                                                 tCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                  >  2:52547/1‑140 (MQ=255)                                                    tCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAat                                                                               >  1:107012/1‑140 (MQ=255)                                                                gccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAg                                                                   >  1:863791/1‑140 (MQ=255)                                                                                  gCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtt                                                 >  1:255770/1‑140 (MQ=255)                                                                                          ctCGCCGGGGGTTGGCTTTCCCAGCCCTGCCAGCAGGCGCAGCGGGGTTCTTTTCCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGt                                         <  2:155319/139‑1 (MQ=255)                                                                                                  gggTGGGCTTTCCCAGCCCCCCCGGCGGGCGCAGCGGGTTGTTTTCCCCCCCCCCGCGCCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                 <  2:107012/139‑1 (MQ=255)                                                                                                       ggCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTAc                            >  1:229816/1‑140 (MQ=255)                                                                                                             tCCAGACCTGCCAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAtt                      >  2:483005/1‑140 (MQ=255)                                                                                                                accccTGCCGGCGGGCGCAGGGGGTTGTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                   <  1:409325/137‑1 (MQ=255)                                                                                                                     cgCCAGCGGGCGCGGGGGGGTTTTTTTCCCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaaca              <  2:255770/139‑1 (MQ=255)                                                                                                                       ccAGCAGGCGCAGCAGGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt            >  1:54427/1‑140 (MQ=255)                                                                                                                                 cagcagGGTGCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCt  >  2:639144/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   ACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCT  >  NC_000913/993755‑994022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑