Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,071,284	A→G	L161P (CTG→CCG)	rutD ←	putative aminoacrylate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,071,284	0	A	G	100.0%	178.1 / NA	50	L161P (CTG→CCG)	rutD	putative aminoacrylate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/30);  total (20/30)

GCGCAGATGATTTGCACCGGGCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTAT  >  NC_000913/1071149‑1071417                                                                                                                                        |                                                                                                                                      gcgcAGATGATTTGCACCGGGCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCg                                                                                                                                   >  1:807444/1‑140 (MQ=255)  cgcAGATGATTTGCACCGGGCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGa                                                                                                                                  >  2:1045610/1‑140 (MQ=255)                    ggCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATc                                                                                                                <  2:545499/140‑1 (MQ=255)                    ggCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATc                                                                                                                >  1:1157010/1‑140 (MQ=255)                    ggCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATc                                                                                                                >  1:235345/1‑140 (MQ=255)                         cGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTc                                                                                                           >  1:102949/1‑140 (MQ=255)                           gATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTcgg                                                                                                         <  2:90451/140‑1 (MQ=255)                           gATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTcgg                                                                                                         <  2:259131/140‑1 (MQ=255)                              gCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTcggcgg                                                                                                      <  2:390577/140‑1 (MQ=255)                              gCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTcggcgg                                                                                                      <  2:933956/140‑1 (MQ=255)                                gATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGa                                                                                                    <  1:1045610/140‑1 (MQ=255)                                gATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGa                                                                                                    <  2:235345/140‑1 (MQ=255)                                gATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGa                                                                                                    >  1:527799/1‑140 (MQ=255)                                    cGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATaga                                                                                                <  1:31450/140‑1 (MQ=255)                                      cATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCAACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGAt                                                                                                   >  1:111003/1‑135 (MQ=255)                                      cATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCAACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGAt                                                                                                   <  2:111003/135‑1 (MQ=255)                                            gACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGaacaac                                                                                        >  1:302286/1‑140 (MQ=255)                                             aCTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACg                                                                                       >  1:1101342/1‑140 (MQ=255)                                               tAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGc                                                                                     <  2:336035/140‑1 (MQ=255)                                                 aaGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTg                                                                                   >  2:181679/1‑140 (MQ=255)                                                 aaGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTg                                                                                   <  2:1157010/140‑1 (MQ=255)                                                      aGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGACTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCtt                                                                              >  2:530956/1‑140 (MQ=255)                                                       gcgcGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTg                                                                                   <  1:292740/134‑1 (MQ=255)                                                       gcgcGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTg                                                                                   >  2:292740/1‑134 (MQ=255)                                                        cgcgTTTGATGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTcc                                                                            >  2:139084/1‑140 (MQ=255)                                                        cgcgTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTcc                                                                            >  1:1121075/1‑140 (MQ=255)                                                         gcgTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCa                                                                           >  1:457181/1‑140 (MQ=255)                                                           gTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCAcc                                                                         <  1:285916/140‑1 (MQ=255)                                                            tttGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCAccc                                                                        >  2:785965/1‑140 (MQ=255)                                                                        aaGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTgcgcgc                                                            <  1:793743/140‑1 (MQ=255)                                                                          gTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccg                                                          <  2:313199/140‑1 (MQ=255)                                                                          gTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccg                                                          <  2:457181/140‑1 (MQ=255)                                                                           tCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccgc                                                         <  1:987716/140‑1 (MQ=255)                                                                           tCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccg                                                          >  2:668348/1‑139 (MQ=255)                                                                           tCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccg                                                          <  1:668348/139‑1 (MQ=255)                                                                             gACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccgccg                                                       <  1:180036/140‑1 (MQ=255)                                                                             gACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGcgccgccg                                                       <  1:785965/140‑1 (MQ=255)                                                                                  aGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCtata                                                  <  2:302286/140‑1 (MQ=255)                                                                                  aGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCtata                                                  <  1:139084/140‑1 (MQ=255)                                                                                   gTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATAc                                                 >  2:510608/1‑140 (MQ=255)                                                                                               tGCCCTGAAAATGCACCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTcg                                     <  2:715016/140‑1 (MQ=255)                                                                                                gCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTcgc                                    >  1:857140/1‑140 (MQ=255)                                                                                                 cccTGAAAATGCGCCGGTGGCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTcgcg                                   <  1:530956/140‑1 (MQ=255)                                                                                                     gAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTcgcg                                   >  1:1036210/1‑136 (MQ=255)                                                                                                     gAAAATGCGCCAGCGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTcgcg                                   <  2:1036210/136‑1 (MQ=255)                                                                                                                  tGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAgcggc                  >  1:995282/1‑140 (MQ=255)                                                                                                                  tGCCAGCGCGTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTCCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAgcggc                  <  2:807444/140‑1 (MQ=255)                                                                                                                          cgTCTTCTGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATgg          <  2:1101342/140‑1 (MQ=255)                                                                                                                              ttctGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGt      <  2:102949/140‑1 (MQ=255)                                                                                                                               tctGCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGtt     <  1:813776/140‑1 (MQ=255)                                                                                                                                  gCCTCCGGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTAt  <  2:972891/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GCGCAGATGATTTGCACCGGGCAGCGGATGCGATCCGCATGGTGACTAAAGTCAGCGCGTTTGAGGGCGTTAAGTCGACGCAGTAAATTATTTTTGCCCTGAAAATGCGCCAGTGCCAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTAT  >  NC_000913/1071149‑1071417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑