Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	109,503	A→G	T409A (ACC→GCC)	secA →	protein translocation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	109,503	0	A	G	100.0%	231.1 / NA	65	T409A (ACC→GCC)	secA	protein translocation ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/33);  total (32/33)

GATCCAGAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATACCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCTGG  >  NC_000913/109370‑109638                                                                                                                                      |                                                                                                                                        gATCCAGAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCcgtcg                                                                                                                                   <  1:291170/140‑1 (MQ=255) gATCCAGAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCcgtcg                                                                                                                                   <  1:597255/140‑1 (MQ=255)    ccAGAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCgttg                                                                                                                                <  2:237555/140‑1 (MQ=255)       gAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTc                                                                                                                             >  2:367042/1‑140 (MQ=255)       gAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTc                                                                                                                             >  1:169741/1‑140 (MQ=255)           gAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGAc                                                                                                                         <  1:887288/140‑1 (MQ=255)              aaCCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCaa                                                                                                                      <  1:367042/140‑1 (MQ=255)                  aaaCGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCaa                                                                                                                      >  2:89659/1‑136 (MQ=255)                  aaaCGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCaa                                                                                                                      <  1:89659/136‑1 (MQ=255)                     cGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCa                                                                                                               <  1:361581/140‑1 (MQ=255)                     cGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTCCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCa                                                                                                               <  2:810190/140‑1 (MQ=255)                          gCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCa                                                                                                                       <  1:933993/127‑1 (MQ=255)                          gCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCa                                                                                                                       >  2:933993/1‑127 (MQ=255)                               gATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTa                                                                                                     >  2:685032/1‑140 (MQ=255)                                  cACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAg                                                                                                  >  1:287731/1‑140 (MQ=255)                                    ccTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGAt                                                                                                >  2:618912/1‑140 (MQ=255)                                           gAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCgg                                                                                         >  2:968630/1‑140 (MQ=255)                                            aaCTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGa                                                                                        >  1:542460/1‑140 (MQ=255)                                            aaCTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGa                                                                                        >  2:106857/1‑140 (MQ=255)                                             actactTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGAc                                                                                       <  2:485341/140‑1 (MQ=255)                                               tactTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCt                                                                                     >  1:803820/1‑140 (MQ=255)                                                actTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTg                                                                                    <  2:169741/140‑1 (MQ=255)                                                 ctTCTGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTgg                                                                                   >  2:934427/1‑140 (MQ=255)                                                   tCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTc                                                                                 <  1:1146138/140‑1 (MQ=255)                                                      gTCTGTATGAAAAACTGGCGGGGGTGACCGGGACTGCTGATATCGAAGCTTTCGAATTTTGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCCACCGTCCAATGATTCGTAAAAATTTGCCGGACCTGGGCTAc                                                                              >  2:1146138/1‑140 (MQ=255)                                                      gTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTAc                                                                              >  1:1067235/1‑140 (MQ=255)                                                                      ggCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGaaa                                                              <  1:618912/140‑1 (MQ=255)                                                                                aCCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGc                                                    <  2:669720/140‑1 (MQ=255)                                                                                aCCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGc                                                    <  1:163998/140‑1 (MQ=255)                                                                                aCCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAAATCAGGc                                                    <  1:107536/140‑1 (MQ=255)                                                                                 ccGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCg                                                   >  1:174819/1‑140 (MQ=255)                                                                                  cGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGa                                                  <  2:479210/140‑1 (MQ=255)                                                                                      aCTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAgg                                                     <  2:251286/133‑1 (MQ=255)                                                                                      aCTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAgg                                                     >  1:251286/1‑133 (MQ=255)                                                                                       ctgctgATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCAtt                                             <  1:562338/140‑1 (MQ=255)                                                                                         gctgATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGa                                           <  2:174819/140‑1 (MQ=255)                                                                                         gctgATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGa                                           <  2:287731/140‑1 (MQ=255)                                                                                         gctgATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGa                                           <  1:934427/140‑1 (MQ=255)                                                                                           tgATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAg                                         <  2:109287/140‑1 (MQ=255)                                                                                            gATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGa                                        >  2:411749/1‑140 (MQ=255)                                                                                              tACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGata                                      <  2:542460/140‑1 (MQ=255)                                                                                               aCCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCGATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGatat                                     >  2:1024029/1‑140 (MQ=255)                                                                                               aCCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGc                                                    >  2:808065/1‑125 (MQ=255)                                                                                               aCCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGc                                                    <  1:808065/125‑1 (MQ=255)                                                                                               aCCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGatat                                     >  2:218387/1‑140 (MQ=255)                                                                                               aCCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGatat                                     >  2:630225/1‑140 (MQ=255)                                                                                                 cGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCa                                   >  1:81525/1‑140 (MQ=255)                                                                                                 cGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCa                                   >  2:213537/1‑140 (MQ=255)                                                                                                  gAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCaa                                  <  2:168403/140‑1 (MQ=255)                                                                                                    aGCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAg                                >  2:601329/1‑140 (MQ=255)                                                                                                     gCTTTCGAATTTAGCTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGa                               <  1:547674/140‑1 (MQ=255)                                                                                                                   cTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCCATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAg                 >  2:1051431/1‑140 (MQ=255)                                                                                                                   cTCAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAg                 >  1:1105808/1‑140 (MQ=255)                                                                                                                     cAATCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGc               <  2:803820/140‑1 (MQ=255)                                                                                                                      aaTCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGcc              >  1:609995/1‑140 (MQ=255)                                                                                                                       aTCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCa             >  2:1085963/1‑140 (MQ=255)                                                                                                                        tCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAg            >  1:553062/1‑140 (MQ=255)                                                                                                                        tCTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAg            >  1:398694/1‑140 (MQ=255)                                                                                                                         cTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGc           <  2:553062/140‑1 (MQ=255)                                                                                                                         cTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGc           <  1:1085963/140‑1 (MQ=255)                                                                                                                         cTACAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGc           <  2:398694/140‑1 (MQ=255)                                                                                                                            cAAGCTGGATGCCGTCGTTGTTCCGACCAACCGTCCCATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCgg        <  1:1051431/140‑1 (MQ=255)                                                                                                                                cTGGATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCt    >  1:196245/1‑140 (MQ=255)                                                                                                                                  ggATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCtgg  >  1:29019/1‑140 (MQ=255)                                                                                                                                  ggATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCtgg  >  1:703424/1‑140 (MQ=255)                                                                                                                                  ggATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCtgg  >  1:717457/1‑140 (MQ=255)                                                                                                                                   gATGCCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCt    <  1:683989/137‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GATCCAGAACGAAAACCAAACGCTGGCTTCGATCACCTTCCAGAACTACTTCCGTCTGTATGAAAAACTGGCGGGGATGACCGGTACTGCTGATACCGAAGCTTTCGAATTTAGCTCAATCTACAAGCTGGATACCGTCGTTGTTCCGACCAACCGTCCAATGATTCGTAAAGATCTGCCGGACCTGGTCTACATGACTGAAGCGGAAAAAATTCAGGCGATCATTGAAGATATCAAAGAACGTACTGCGAAAGGCCAGCCGGTGCTGG  >  NC_000913/109370‑109638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑