Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,146,094 C→T D168N (GAT→AAT)  yceF ← m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,146,0940CT100.0% 163.5 / NA 46D168N (GAT→AAT) yceFm(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

AGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACG  >  NC_000913/1145958‑1146224
                                                                                                                                        |                                                                                                                                  
aGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACg                                                                                                                                 <  1:150836/140‑1 (MQ=255)
aGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACg                                                                                                                                 <  2:1079297/140‑1 (MQ=255)
aGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACg                                                                                                                                 <  2:128165/140‑1 (MQ=255)
  gccgccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACg                                                                                                                                 >  2:651654/1‑138 (MQ=255)
  gccgccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACg                                                                                                                                 <  1:651654/138‑1 (MQ=255)
  gccgccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGcc                                                                                                                               >  2:417205/1‑140 (MQ=255)
            ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACg                                                                                                                     >  1:941553/1‑140 (MQ=255)
               aTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGc                                                                                                                    >  1:400928/1‑138 (MQ=255)
               aTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGc                                                                                                                    <  2:400928/138‑1 (MQ=255)
                       aTGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCg                                                                                                          >  2:762330/1‑140 (MQ=255)
                              gTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGcc                                                                                                   <  1:417205/140‑1 (MQ=255)
                                  ccACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAAt                                                                                               >  2:61679/1‑140 (MQ=255)
                                      aTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCtt                                                                                           <  2:947936/140‑1 (MQ=255)
                                       tCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTc                                                                                          >  1:677511/1‑140 (MQ=255)
                                       tCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTc                                                                                          >  2:1137934/1‑140 (MQ=255)
                                           gcgcAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCActc                                                                                      <  1:783338/140‑1 (MQ=255)
                                            cgcAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCActct                                                                                     >  2:304349/1‑140 (MQ=255)
                                               aaaaaaCTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTaa                                                                                  >  2:201440/1‑140 (MQ=255)
                                                aaaaaCTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTaaa                                                                                 <  1:201440/140‑1 (MQ=255)
                                                          ccATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcgcg                                                                       <  2:258987/140‑1 (MQ=255)
                                                          ccATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcgcg                                                                       <  2:245135/140‑1 (MQ=255)
                                                          ccATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcgcg                                                                       <  1:1126719/140‑1 (MQ=255)
                                                          ccATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcgcg                                                                       <  1:1137934/140‑1 (MQ=255)
                                                          ccATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcgcg                                                                       <  1:554891/140‑1 (MQ=255)
                                                             tCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAg                                                                    >  2:261100/1‑140 (MQ=255)
                                                               aGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTg                                                                  <  1:237468/140‑1 (MQ=255)
                                                                gCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGc                                                                 <  1:61679/140‑1 (MQ=255)
                                                                        ttcccttcccGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATg                                                         <  2:651470/140‑1 (MQ=255)
                                                                                  gACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGc                                               >  2:450755/1‑140 (MQ=255)
                                                                                   aCGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCa                                              <  1:832816/140‑1 (MQ=255)
                                                                                        aCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTaa                                         >  1:900284/1‑140 (MQ=255)
                                                                                          aTCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGt                                           >  1:795444/1‑136 (MQ=255)
                                                                                          aTCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGt                                           <  2:795444/136‑1 (MQ=255)
                                                                                                    gcgcGATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTcc                             >  2:99825/1‑140 (MQ=255)
                                                                                                     cgcgATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCg                            >  1:850914/1‑140 (MQ=255)
                                                                                                     cgcgATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCg                            >  1:871487/1‑140 (MQ=255)
                                                                                                     cgcgATTAGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCg                            >  1:113616/1‑140 (MQ=255)
                                                                                                            aGCGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCt                     <  1:215918/140‑1 (MQ=255)
                                                                                                              cGGTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCa                   >  2:802126/1‑140 (MQ=255)
                                                                                                               ggTAAACCAACCAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAg                  >  1:1064370/1‑140 (MQ=255)
                                                                                                                   aaccaaccAGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGa              <  2:256335/140‑1 (MQ=255)
                                                                                                                        accaGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGaa         >  1:762876/1‑140 (MQ=255)
                                                                                                                           aGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATg      <  2:1020430/140‑1 (MQ=255)
                                                                                                                           aGCGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATg      <  2:677511/140‑1 (MQ=255)
                                                                                                                             cGTGTTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGa    >  2:37918/1‑140 (MQ=255)
                                                                                                                               tgtTAGGATTACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACg  >  2:640343/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                  
AGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACG  >  NC_000913/1145958‑1146224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑