Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,160,689	T→C	N288S (AAC→AGC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,160,689	0	T	C	100.0%	261.8 / NA	72	N288S (AAC→AGC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/39);  total (33/39)

CTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGTTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAA  >  NC_000913/1160555‑1160822                                                                                                                                       |                                                                                                                                      cTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAActgctgctg                                                                                                                                  <  2:766290/140‑1 (MQ=255) cTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAActgctgctg                                                                                                                                  >  1:740836/1‑140 (MQ=255)   tCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTgctgctgct                                                                                                                                <  1:1088284/140‑1 (MQ=255)      gagTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGcc                                                                                                                             >  1:840780/1‑140 (MQ=255)                cATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTc                                                                                                                   <  2:336427/140‑1 (MQ=255)                cATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTc                                                                                                                   >  2:874352/1‑140 (MQ=255)                  ttCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCa                                                                                                                 <  1:572759/140‑1 (MQ=255)                   tCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAg                                                                                                                <  2:1068731/140‑1 (MQ=255)                     aGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAgcg                                                                                                              >  2:112384/1‑140 (MQ=255)                      gtgtCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAgcgc                                                                                                             >  1:217893/1‑140 (MQ=255)                       tgtCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGcgcg                                                                                                            <  2:438459/140‑1 (MQ=255)                         tCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGt                                                                                                          >  1:689183/1‑140 (MQ=255)                          cGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTa                                                                                                         >  1:724531/1‑140 (MQ=255)                          cGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTa                                                                                                         >  1:897209/1‑140 (MQ=255)                          cGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTa                                                                                                         >  1:82126/1‑140 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       <  2:542789/140‑1 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       >  1:566379/1‑140 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       >  2:645850/1‑140 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       <  2:217893/140‑1 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       <  2:897209/140‑1 (MQ=255)                            cTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTaaa                                                                                                       <  1:1040339/140‑1 (MQ=255)                                 cAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGcc                                                                                                  <  1:112384/140‑1 (MQ=255)                                  aGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGccc                                                                                                 <  1:472631/140‑1 (MQ=255)                                      aTCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAgg                                                                                             <  1:645850/140‑1 (MQ=255)                                       tCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGt                                                                                            <  2:789395/140‑1 (MQ=255)                                        cAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTa                                                                                           <  2:352617/140‑1 (MQ=255)                                         aGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGTCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAg                                                                                          <  2:130391/140‑1 (MQ=255)                                          gCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAgg                                                                                         >  1:149273/1‑140 (MQ=255)                                          gCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAgg                                                                                         <  1:271435/140‑1 (MQ=255)                                           cAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAggg                                                                                        <  2:330806/140‑1 (MQ=255)                                           cAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAggg                                                                                        <  2:840780/140‑1 (MQ=255)                                             aaCTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGACCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCt                                                                                      >  1:555392/1‑140 (MQ=255)                                              aCTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTa                                                                                     >  1:1026654/1‑140 (MQ=255)                                               ctgctgCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTAt                                                                                    <  2:326391/140‑1 (MQ=255)                                               ctgctgCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTAt                                                                                    <  1:207368/140‑1 (MQ=255)                                               ctgctgCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTAt                                                                                    >  1:363504/1‑140 (MQ=255)                                                  ctgctTCAGGGTCGTAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTaa                                                                                 <  2:583615/140‑1 (MQ=255)                                                     ctTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTACGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACAt                                                                              >  2:736476/1‑140 (MQ=255)                                                          gggTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATg                                                                         >  1:244376/1‑140 (MQ=255)                                                            gTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATgcg                                                                       <  2:562398/140‑1 (MQ=255)                                                            gTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATgcg                                                                       <  2:841869/140‑1 (MQ=255)                                                                     aCCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTc                                                              >  1:383177/1‑140 (MQ=255)                                                                       cTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGt                                                            >  1:580537/1‑140 (MQ=255)                                                                          gttgATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAAc                                                         >  2:7496/1‑140 (MQ=255)                                                                                ctctTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTg                                                   >  1:893595/1‑140 (MQ=255)                                                                                ctctTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTg                                                   >  2:21560/1‑140 (MQ=255)                                                                                   tttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGaaa                                                <  1:21560/140‑1 (MQ=255)                                                                                    ttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAg                                               <  2:419032/140‑1 (MQ=255)                                                                                    ttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAg                                               <  2:1029189/140‑1 (MQ=255)                                                                                    ttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAg                                               <  1:230473/140‑1 (MQ=255)                                                                                    ttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAg                                               <  1:31649/140‑1 (MQ=255)                                                                                    ttATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAg                                               <  1:7496/140‑1 (MQ=255)                                                                                        cGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGcgtc                                           <  2:363504/140‑1 (MQ=255)                                                                                          ttGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTa                                                           <  2:237374/122‑1 (MQ=255)                                                                                          ttGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTa                                                           >  1:237374/1‑122 (MQ=255)                                                                                           tGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTa                                        >  2:312666/1‑140 (MQ=255)                                                                                            gTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTaa                                       >  1:498839/1‑140 (MQ=255)                                                                                             tACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGaa                                                 >  2:795802/1‑129 (MQ=255)                                                                                             tACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGaa                                                 <  1:795802/129‑1 (MQ=255)                                                                                              aCGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGa                                     <  2:1026654/140‑1 (MQ=255)                                                                                              aCGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGa                                     <  2:570646/140‑1 (MQ=255)                                                                                              aCGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGa                                     <  2:593432/140‑1 (MQ=255)                                                                                               cGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGAt                                    >  1:930551/1‑140 (MQ=255)                                                                                                 cccAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGcgtc                                           >  2:94554/1‑131 (MQ=255)                                                                                                 cccAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGcgtc                                           <  1:94554/131‑1 (MQ=255)                                                                                                    aGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCt                               >  1:162484/1‑140 (MQ=255)                                                                                                       cAGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTaaa                            >  2:310299/1‑140 (MQ=255)                                                                                                        aGGTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAAt                           <  2:82126/140‑1 (MQ=255)                                                                                                          gTGCGGTACTGCGTGCGCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGACTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCa                         >  1:394006/1‑140 (MQ=255)                                                                                                                    gcgtgcgCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACaa               >  2:769480/1‑140 (MQ=255)                                                                                                                    gcgtgcgCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACaa               <  2:930551/140‑1 (MQ=255)                                                                                                                    gcgtgcgCGACCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACaa               <  2:555392/140‑1 (MQ=255)                                                                                                                       tgcgCGATCGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGc            >  2:1074627/1‑140 (MQ=255)                                                                                                                               cGTAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGagaag    >  2:133473/1‑140 (MQ=255)                                                                                                                                 tAACTGCTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAgaagaa  <  1:310299/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAGCAAACTGCTGCTTCAGGGTCATAAAGACCTTGTTGATCTCTTTATCGTTGTACGCCCAGTCAGGTGCGGTACTGCGTGCGCGATCGTAACTGTTGCTGCTGCCATCAGTATTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAA  >  NC_000913/1160555‑1160822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑