Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,791	A→G	A13A (GCT→GCC)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,791	0	A	G	100.0%	119.8 / NA	36	A13A (GCT→GCC)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/10);  total (26/10)

GTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCA  >  NC_000913/1182657‑1182923                                                                                                                                       |                                                                                                                                     gTCAAATAGATACCCTTAATCCCGTTTTCTTTGGTGAACTGTTCAAGCAGTCCGGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGcccc                                                                                                                                 <  2:971913/140‑1 (MQ=255)          aTAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAg                                                                                                                        >  2:1033493/1‑140 (MQ=255)            aaCCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCa                                                                                                                      >  1:971913/1‑140 (MQ=255)              ccTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTTTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAgg                                                                                                                    <  1:447904/140‑1 (MQ=255)                   aTACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCg                                                                                                               >  1:57745/1‑140 (MQ=255)                        ggTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGAcc                                                                                                          >  2:185389/1‑140 (MQ=255)                         gTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCa                                                                                                         >  1:1081971/1‑140 (MQ=255)                         gTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCa                                                                                                         >  2:1005715/1‑140 (MQ=255)                            tCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCAttt                                                                                                      >  1:1061943/1‑140 (MQ=255)                            tCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCAttt                                                                                                      >  1:765643/1‑140 (MQ=255)                             cTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCAtttt                                                                                                     <  2:1081971/140‑1 (MQ=255)                                tGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTc                                                                                                  <  1:696396/140‑1 (MQ=255)                                tGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAAAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCGGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTc                                                                                                  <  2:1122415/140‑1 (MQ=255)                                       cTGTTCAGGCGGTCCTGGCGGCACGTACTCGGTCCAGTTGTAAAAATACGGCGTGTTGTTGTCAGCGGCGGGGGCGGCGCTCTGGCCAAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           <  2:765643/140‑1 (MQ=255)                                       cTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCGGTGCCAGGGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           <  2:322420/140‑1 (MQ=255)                                       cTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           <  1:142613/140‑1 (MQ=255)                                       cTGTTAAAGCAGCCCGGGCGGCACGTCCTCGGTCCGGTTGTAAAAATACAGCGTGTTGTTTTCAGCGGCGGGAGCGGCGCTCAGGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           <  2:1061943/140‑1 (MQ=255)                                          ttCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTc                                                                                        >  1:741163/1‑140 (MQ=255)                                           tCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATGGGGGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTcc                                                                                       <  2:1073812/140‑1 (MQ=255)                                               gCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCtg                                                                                   >  2:883704/1‑140 (MQ=255)                                                  gTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTgtgt                                                                                >  1:1308/1‑140 (MQ=255)                                                    ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTaa                                                                                            >  1:598539/1‑126 (MQ=255)                                                    ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTaa                                                                                            <  2:598539/126‑1 (MQ=255)                                                    ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTa                                                                              >  1:374446/1‑140 (MQ=255)                                                         cggcACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           >  1:889263/1‑122 (MQ=255)                                                         cggcACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAAc                                                                                           <  2:889263/122‑1 (MQ=255)                                                              cGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGttt                                                                    >  2:104049/1‑140 (MQ=255)                                                                  cTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATc                                                                >  2:971591/1‑140 (MQ=255)                                                                    cGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCAc                                                              <  1:1033493/140‑1 (MQ=255)                                                                      gTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGa                                                            >  2:147081/1‑140 (MQ=255)                                                                                  aaaTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCGGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctgg                                                <  1:674803/140‑1 (MQ=255)                                                                                  aaaTACAGCGTGTTGTTGTCAGCGGCGTGAGCGCCGCTCATGCCCGGTGCCAGGCCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctgg                                                <  2:329328/140‑1 (MQ=255)                                                                                   aaTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggc                                               <  2:124200/140‑1 (MQ=255)                                                                                    aTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggct                                              >  2:237063/1‑140 (MQ=255)                                                                                    aTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggct                                              <  1:883704/140‑1 (MQ=255)                                                                                        ggCGTGTTTTTGTCACCGGCGTGGGCGGCGCTCATGCCCAGTGCCAGGGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa                                          <  2:57745/139‑1 (MQ=255)                                                                                        aGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa                                          >  2:836988/1‑140 (MQ=255)                                                                                         gCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCaa                                         >  2:683793/1‑140 (MQ=255)                                                                                         gCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCaa                                         <  2:1308/140‑1 (MQ=255)                                                                                         gCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCaa                                         >  2:305925/1‑140 (MQ=255)                                                                                              ttgttgtCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAAcc                                    >  1:871137/1‑140 (MQ=255)                                                                                                      aTCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAg                            >  2:382180/1‑140 (MQ=255)                                                                                                        gggggTGAGCGGCGCTCAGGCCAGGTGCCGGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCg                          <  2:374446/136‑1 (MQ=255)                                                                                                        cGGCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCg                          <  1:971591/140‑1 (MQ=255)                                                                                                         ggCGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGa                         >  2:828911/1‑140 (MQ=255)                                                                                                           cGTGAGCGGCGCTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACa                       <  1:185389/140‑1 (MQ=255)                                                                                                               ggcggcgCTCATGCCCAGTGCCAGGGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAc                   <  1:75433/139‑1 (MQ=255)                                                                                                               ggcggcgCTCATGCCCAGGGCCGGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAc                   <  2:741163/139‑1 (MQ=255)                                                                                                                    cgcTCATGCCCAGTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTa              >  1:919471/1‑140 (MQ=255)                                                                                                                           ccccGGTGCCGGGCCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGt       <  1:113940/139‑1 (MQ=255)                                                                                                                                gTGCCGGGGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCa  <  2:881738/140‑1 (MQ=255)                                                                                                                                gTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAcc                  <  2:344891/124‑1 (MQ=255)                                                                                                                                gTGCCAGGGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAcc                  >  1:344891/1‑124 (MQ=255)                                                                                                                                       |                                                                                                                                     GTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCA  >  NC_000913/1182657‑1182923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑