Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,786	G→A	A30A (GCC→GCT)	prs ←	ribose‑phosphate diphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,786	0	G	A	100.0%	219.6 / NA	61	A30A (GCC→GCT)	prs	ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/29);  total (32/29)

ATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGA  >  NC_000913/1261658‑1261912                                                                                                                                 |                                                                                                                               aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAg                                                                                                                     >  2:929084/1‑140 (MQ=255)   aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTg                                                                                                                   <  2:297603/140‑1 (MQ=255)       aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGc                                                                                                                         >  2:371272/1‑130 (MQ=255)       aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGc                                                                                                                         <  1:371272/130‑1 (MQ=255)         tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAgtgt                                                                                                             >  2:233811/1‑140 (MQ=255)          aaTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTa                                                                                                            >  1:351788/1‑140 (MQ=255)                aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                      >  1:795240/1‑140 (MQ=255)                aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                      >  2:174010/1‑140 (MQ=255)                aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                      <  1:174010/140‑1 (MQ=255)                 tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                     >  2:852359/1‑140 (MQ=255)                 tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                     >  2:1143477/1‑140 (MQ=255)                  cAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGt                                                                                                    <  2:131931/140‑1 (MQ=255)                  cAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGt                                                                                                    <  1:877406/140‑1 (MQ=255)                         tCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCaaa                                                                                             >  2:514163/1‑139 (MQ=255)                         tCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                             <  2:213213/140‑1 (MQ=255)                            ttAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACg                                                                                          <  2:519349/140‑1 (MQ=255)                             tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGt                                                                                         <  2:879476/140‑1 (MQ=255)                              agtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                        >  2:1098928/1‑140 (MQ=255)                              agtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                        >  2:10427/1‑140 (MQ=255)                                   gggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCt                                                                                   >  2:183809/1‑140 (MQ=255)                                     gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                 >  2:642046/1‑140 (MQ=255)                                     gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                 >  1:358639/1‑140 (MQ=255)                                      cacaAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGt                                                                                >  1:777387/1‑140 (MQ=255)                                          aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                            >  2:153758/1‑140 (MQ=255)                                          aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                            >  2:593221/1‑140 (MQ=255)                                               aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCggg                                                                          >  1:965333/1‑137 (MQ=255)                                               aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCggg                                                                          <  2:965333/137‑1 (MQ=255)                                                   gatgatGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGCACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGt                                                                   >  1:740640/1‑140 (MQ=255)                                                             atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                         >  2:334473/1‑140 (MQ=255)                                                             atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                         <  2:56179/140‑1 (MQ=255)                                                             atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                         >  1:306397/1‑140 (MQ=255)                                                             atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                         <  1:183809/140‑1 (MQ=255)                                                              tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaaa                                                        <  1:153758/140‑1 (MQ=255)                                                                tCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                      <  1:1161852/140‑1 (MQ=255)                                                                  accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCt                                                    <  1:10427/140‑1 (MQ=255)                                                                  accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAGCt                                                    >  1:1017775/1‑140 (MQ=255)                                                                  accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAGCt                                                    >  1:175094/1‑140 (MQ=255)                                                                       cgcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                               >  1:903601/1‑140 (MQ=255)                                                                        gcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCatat                                              >  1:325234/1‑140 (MQ=255)                                                                         cgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTATAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                             >  2:504548/1‑140 (MQ=255)                                                                         cgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                             >  1:1036090/1‑140 (MQ=255)                                                                         cgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                             >  2:728654/1‑140 (MQ=255)                                                                         cgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                             >  2:587643/1‑140 (MQ=255)                                                                          gTACATTTTCATTAATTCGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa                                            <  2:1036090/140‑1 (MQ=255)                                                                             cATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGc                                         <  2:1017775/140‑1 (MQ=255)                                                                              aTTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCa                                        <  1:642046/140‑1 (MQ=255)                                                                                 ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGGTACCAGCAAAAAGCTTCATATCAGGCACga                                     >  2:123620/1‑140 (MQ=255)                                                                                     ttAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                 <  2:843974/140‑1 (MQ=255)                                                                                        aTTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                 <  2:207434/137‑1 (MQ=255)                                                                                        aTTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                 >  1:207434/1‑137 (MQ=255)                                                                                           tGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTc                           <  2:903601/140‑1 (MQ=255)                                                                                               cGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                       <  1:852359/140‑1 (MQ=255)                                                                                               cGCTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                       <  1:1143477/140‑1 (MQ=255)                                                                                                 cTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAt                     <  2:175094/140‑1 (MQ=255)                                                                                                 cTGACTTCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAt                     <  1:1098928/140‑1 (MQ=255)                                                                                                      ttCGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTc                <  2:358639/140‑1 (MQ=255)                                                                                                        cGCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa              <  2:795240/140‑1 (MQ=255)                                                                                                         gCCATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt             <  1:115068/140‑1 (MQ=255)                                                                                                          ccATCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAtt            <  2:306397/140‑1 (MQ=255)                                                                                                            aTCGCTAAAGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtgg          >  2:934766/1‑140 (MQ=255)                                                                                                                    aGCGACCTACAGCAGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa  <  2:777387/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                               ATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGA  >  NC_000913/1261658‑1261912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑