Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,281,216	G→A	V451V (GTG→GTA)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,281,216	0	G	A	100.0%	272.0 / NA	77	V451V (GTG→GTA)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/44);  total (33/44)

CGCGACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCA  >  NC_000913/1281079‑1281349                                                                                                                                          |                                                                                                                                      cgcgACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtact                                                                                                                                     >  2:165660/1‑140 (MQ=255) cgcgACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtact                                                                                                                                     >  1:130320/1‑140 (MQ=255)  gcgACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtactg                                                                                                                                    >  2:413595/1‑140 (MQ=255)   cgACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtactgc                                                                                                                                   <  1:76659/140‑1 (MQ=255)      cGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtactgctgc                                                                                                                                >  2:960696/1‑140 (MQ=255)      cGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGtactgctgc                                                                                                                                >  2:348146/1‑140 (MQ=255)         cAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGcaca                                                                                                                             <  2:927059/140‑1 (MQ=255)           aaaCTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACaaa                                                                                                                           >  2:913624/1‑140 (MQ=255)           aaaCTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACaaa                                                                                                                           <  2:898841/140‑1 (MQ=255)               tGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGG‑AAACGTACTGCTGCACAAACTGcc                                                                                                                      <  2:364774/140‑1 (MQ=255)                 gCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCg                                                                                                                     >  2:458447/1‑140 (MQ=255)                      gaaACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGaa                                                                                                                <  1:214947/140‑1 (MQ=255)                       aaaCCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGaaa                                                                                                               <  1:960696/140‑1 (MQ=255)                          ccGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGc                                                                                                            <  1:835796/140‑1 (MQ=255)                                 gCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAAc                                                                                                     <  1:1109210/140‑1 (MQ=255)                                 gCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAAc                                                                                                     >  1:78061/1‑140 (MQ=255)                                           ctgctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATgg                                                                                           <  1:165660/140‑1 (MQ=255)                                              ctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGgcag                                                                                        <  2:1163005/140‑1 (MQ=255)                                              ctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGgcag                                                                                        <  1:202863/140‑1 (MQ=255)                                              ctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTACCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGgcag                                                                                        >  2:423351/1‑140 (MQ=255)                                                gggTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGgcagca                                                                                      <  1:468151/140‑1 (MQ=255)                                                     gCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGcc                                                                                 <  1:986316/140‑1 (MQ=255)                                                     gCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGcc                                                                                 <  1:348146/140‑1 (MQ=255)                                                      ccAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGccc                                                                                >  2:397493/1‑140 (MQ=255)                                                          gATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGt                                                                            <  1:907200/140‑1 (MQ=255)                                                           aTGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTg                                                                           >  2:603812/1‑140 (MQ=255)                                                             gagaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         <  2:189025/140‑1 (MQ=255)                                                             gagaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         <  2:985300/140‑1 (MQ=255)                                                             gagaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         <  2:287119/140‑1 (MQ=255)                                                             gagaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         <  2:868938/140‑1 (MQ=255)                                                              agaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGacc                                                                        <  1:321189/140‑1 (MQ=255)                                                               gaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGacca                                                                       >  2:947039/1‑140 (MQ=255)                                                               gaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         <  2:340512/138‑1 (MQ=255)                                                               gaTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGac                                                                         >  1:340512/1‑138 (MQ=255)                                                                aTCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGaccac                                                                      <  2:997596/140‑1 (MQ=255)                                                                   gCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGt                                                                   >  2:849139/1‑140 (MQ=255)                                                                    cTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGtt                                                                  >  1:1087231/1‑140 (MQ=255)                                                                    cTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGtt                                                                  >  2:729475/1‑140 (MQ=255)                                                                    cTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGtt                                                                  >  2:62378/1‑140 (MQ=255)                                                                     tGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGttt                                                                 <  2:8749/140‑1 (MQ=255)                                                                     tGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGttt                                                                 <  2:78061/140‑1 (MQ=255)                                                                             gCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTg                                                         >  2:184772/1‑140 (MQ=255)                                                                              cTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGTTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGa                                                        >  1:309766/1‑140 (MQ=255)                                                                                 cccGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGc                                                     >  1:334210/1‑140 (MQ=255)                                                                                   cGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTg                                                   >  1:395321/1‑140 (MQ=255)                                                                                      aCTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCa                                                <  1:458447/140‑1 (MQ=255)                                                                                        tttGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCaaa                                              >  2:369041/1‑140 (MQ=255)                                                                                          tggtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGTTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACt                                            <  2:309766/140‑1 (MQ=255)                                                                                           ggtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTa                                           <  1:184772/140‑1 (MQ=255)                                                                                            gtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTAc                                          >  2:436204/1‑140 (MQ=255)                                                                                            gtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTAc                                          >  2:268076/1‑140 (MQ=255)                                                                                            gtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTAc                                          <  1:423351/140‑1 (MQ=255)                                                                                            gtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTAc                                          <  2:395321/140‑1 (MQ=255)                                                                                             tggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACg                                         <  2:1087231/140‑1 (MQ=255)                                                                                                  aCGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTg                                    >  1:242087/1‑140 (MQ=255)                                                                                                  aCGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTg                                    >  1:359463/1‑140 (MQ=255)                                                                                                    ggCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTa                                           <  2:357229/131‑1 (MQ=255)                                                                                                    ggCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTa                                           >  1:357229/1‑131 (MQ=255)                                                                                                    ggCACGGAACACTTCAACAAAGTGGAACTGCAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGa                                  >  1:911513/1‑140 (MQ=255)                                                                                                      cACGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAAc                                >  2:34917/1‑140 (MQ=255)                                                                                                       aCGGAACACTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACg                               >  1:24939/1‑140 (MQ=255)                                                                                                            acacTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGcc                          <  1:1153787/140‑1 (MQ=255)                                                                                                               cTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGa                       <  1:913624/140‑1 (MQ=255)                                                                                                               cTTCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGa                       <  2:598442/140‑1 (MQ=255)                                                                                                                ttCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGaa                      <  1:413595/140‑1 (MQ=255)                                                                                                                 tCAACAAAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAGACTACGGTCTGGAACGTGGCCTGAac                     <  1:947039/140‑1 (MQ=255)                                                                                                                     caaAGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgacg                 >  1:695413/1‑140 (MQ=255)                                                                                                                        aGTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTa              <  2:359463/140‑1 (MQ=255)                                                                                                                         gTGGAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTaa             >  2:118291/1‑140 (MQ=255)                                                                                                                            gAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACtg          >  1:915905/1‑140 (MQ=255)                                                                                                                            gAACTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACtg          >  2:428181/1‑140 (MQ=255)                                                                                                                             aaCTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgac                  >  1:298613/1‑131 (MQ=255)                                                                                                                             aaCTGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgac                  <  2:298613/131‑1 (MQ=255)                                                                                                                                tGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCa      <  2:695413/140‑1 (MQ=255)                                                                                                                                tGGAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCa      <  2:242087/140‑1 (MQ=255)                                                                                                                                 ggAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCaa     <  2:410534/140‑1 (MQ=255)                                                                                                                                  gAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc    >  2:144106/1‑140 (MQ=255)                                                                                                                                  gAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc    >  2:1140628/1‑140 (MQ=255)                                                                                                                                  gAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc    <  1:62378/140‑1 (MQ=255)                                                                                                                                  gAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc    <  1:118291/140‑1 (MQ=255)                                                                                                                                  gAAAACGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc    <  2:24939/140‑1 (MQ=255)                                                                                                                                   aaaaCGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAcc   <  1:34917/140‑1 (MQ=255)                                                                                                                                    aaaCGTACTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCa  >  2:523581/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CGCGACGGCAAAACTGGCGAAGAAACCGAGCTGCAACTGAGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCA  >  NC_000913/1281079‑1281349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑