Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	135,426	A→G	S53P (TCA→CCA)	speD ←	S‑adenosylmethionine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	135,426	0	A	G	100.0%	285.5 / NA	79	S53P (TCA→CCA)	speD	S‑adenosylmethionine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/40);  total (39/40)

TTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGACAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTA  >  NC_000913/135295‑135556                                                                                                                                    |                                                                                                                                   ttCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGAttt                                                                                                                            >  2:776622/1‑140 (MQ=255) ttCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGAttt                                                                                                                            <  1:776622/140‑1 (MQ=255) ttCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGAttt                                                                                                                            <  1:332733/140‑1 (MQ=255)      ttttGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTc                                                                                                                       <  2:74904/140‑1 (MQ=255)         tGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGa                                                                                                                    >  2:521391/1‑140 (MQ=255)           tCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACg                                                                                                                  >  1:187482/1‑140 (MQ=255)             gATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGt                                                                                                                <  1:50601/140‑1 (MQ=255)             gATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGt                                                                                                                <  1:470234/140‑1 (MQ=255)             gATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGt                                                                                                                <  2:364108/140‑1 (MQ=255)                gAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTgg                                                                                                             >  2:545054/1‑140 (MQ=255)                gAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTgg                                                                                                             >  2:402441/1‑140 (MQ=255)                          tCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATagag                                                                                                   <  1:521977/140‑1 (MQ=255)                           cAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGt                                                                                                  >  2:371099/1‑140 (MQ=255)                           cAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGt                                                                                                  >  2:669791/1‑140 (MQ=255)                             aCCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTc                                                                                                >  2:303591/1‑140 (MQ=255)                             aCCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTc                                                                                                >  1:1057796/1‑140 (MQ=255)                              ccGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCa                                                                                               <  1:371099/140‑1 (MQ=255)                              ccGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCa                                                                                               <  1:736045/140‑1 (MQ=255)                                   tcttcACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGat                                                                                          <  2:1077487/140‑1 (MQ=255)                                   tcttcACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGat                                                                                          <  2:1021790/140‑1 (MQ=255)                                      tcactcacCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGAtata                                                                                       >  2:598457/1‑140 (MQ=255)                                       cactcacCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATaa                                                                                      <  2:1140135/140‑1 (MQ=255)                                       cactcacCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATaa                                                                                      <  2:748922/140‑1 (MQ=255)                                       cactcacCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATaa                                                                                      <  1:144199/140‑1 (MQ=255)                                             ccAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAACGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAata                                                                                <  2:1057796/140‑1 (MQ=255)                                                 aaTAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAAc                                                                            >  1:846667/1‑140 (MQ=255)                                                    aGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCTCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGt                                                                         >  1:540159/1‑140 (MQ=255)                                                      tGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTcg                                                                       <  1:1132798/140‑1 (MQ=255)                                                      tGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTcg                                                                       >  2:1087670/1‑140 (MQ=255)                                                          gCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGct                                                                   >  2:1037953/1‑140 (MQ=255)                                                           cTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGctc                                                                  >  1:207277/1‑140 (MQ=255)                                                           cTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGctc                                                                  >  1:180295/1‑140 (MQ=255)                                                           cTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGctc                                                                  >  1:839134/1‑140 (MQ=255)                                                              gCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTc                                                               >  2:193885/1‑140 (MQ=255)                                                              gCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTc                                                               >  2:993655/1‑140 (MQ=255)                                                                aCCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCgg                                                             <  2:420251/140‑1 (MQ=255)                                                                aCCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCgg                                                             <  2:839134/140‑1 (MQ=255)                                                                    tgtgGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGt                                                         <  1:402441/140‑1 (MQ=255)                                                                    tgtgGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGt                                                         >  1:327787/1‑140 (MQ=255)                                                                    tgtgGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGt                                                         >  2:303696/1‑140 (MQ=255)                                                                    tgtgGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGt                                                         >  2:854738/1‑140 (MQ=255)                                                                      tgGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGttt                                                       <  2:640848/140‑1 (MQ=255)                                                                       ggTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCgg                                                             <  2:734822/133‑1 (MQ=255)                                                                       ggTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCgg                                                             >  1:734822/1‑133 (MQ=255)                                                                        gTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTg                                                     <  1:993655/140‑1 (MQ=255)                                                                          tCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGc                                                   <  1:521901/140‑1 (MQ=255)                                                                            gTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGt                                                 <  1:669791/140‑1 (MQ=255)                                                                              aaTCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAg                                               <  2:951522/140‑1 (MQ=255)                                                                                 ccTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAg                                            <  1:193885/140‑1 (MQ=255)                                                                                   tGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGat                                          <  2:846667/140‑1 (MQ=255)                                                                                     gcgggcgATTCTAAGAATATTAGCCCCGATAATGGAACATGTTTCTGGCAGGATTTCGGTCAGACGGTTGGGATTATAGAGTTCATCGCTATAAGCAATATAACCGTCGCGCCCTTCGGCAGATTTGGCGTAGCACatat                                        >  2:1073258/1‑140 (MQ=255)                                                                                     gcgggcgATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATGAGCAATATAACCGTCGCGCTCTTGGGCAGTTTTGGCGTAGCAGatat                                        >  2:289172/1‑140 (MQ=255)                                                                                     gcgggcgATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTTGCGTAGCAGatat                                        >  2:1165551/1‑140 (MQ=255)                                                                                     gcgggcgATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGatat                                        >  1:891185/1‑140 (MQ=255)                                                                                     gcgggcgATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGatat                                        >  1:68110/1‑140 (MQ=255)                                                                                      cgggcgATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATc                                       >  1:971757/1‑140 (MQ=255)                                                                                                 aaGAATATTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACaaa                            <  1:854738/140‑1 (MQ=255)                                                                                                       atTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCa                      >  1:521498/1‑140 (MQ=255)                                                                                                       atTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCa                      >  2:550044/1‑140 (MQ=255)                                                                                                       atTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCa                      >  1:221251/1‑140 (MQ=255)                                                                                                       atTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCa                      >  1:567183/1‑140 (MQ=255)                                                                                                       atTAGCCCCGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCa                      >  1:512370/1‑140 (MQ=255)                                                                                                              ccGATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACtttt               >  1:1032838/1‑140 (MQ=255)                                                                                                                gATAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTgg             >  1:139419/1‑140 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  1:550044/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  2:327787/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  1:598457/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  1:1087670/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            >  1:1109583/1‑140 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  1:763245/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            <  2:558553/140‑1 (MQ=255)                                                                                                                 aTAATGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGt            >  2:534193/1‑140 (MQ=255)                                                                                                                    aTGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAg         <  2:540159/140‑1 (MQ=255)                                                                                                                    aTGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAg         <  2:971757/140‑1 (MQ=255)                                                                                                                     tGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGa        <  2:130683/140‑1 (MQ=255)                                                                                                                     tGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGa        <  2:139419/140‑1 (MQ=255)                                                                                                                     tGGAACAGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGa        <  2:187482/140‑1 (MQ=255)                                                                                                                           aGGTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGAttatta  >  1:999958/1‑140 (MQ=255)                                                                                                                             gTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAg         >  2:725129/1‑131 (MQ=255)                                                                                                                             gTTTCTGGCAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAg         <  1:725129/131‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   TTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGCGGGCGATGTTAAGAATATTAGCCCCGATAATGGAACAGGTTTCTGACAGGATTTCGGTCAGACGGTTGGCATTATAGAGTTCATCGATATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTA  >  NC_000913/135295‑135556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑