Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,421	A→G	S114P (TCA→CCA)	ycjD ←	DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,421	0	A	G	100.0%	189.0 / NA	54	S114P (TCA→CCA)	ycjD	DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCAACTGC  >  NC_000913/1351285‑1351556                                                                                                                                         |                                                                                                                                        tACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCcg                                                                                                                                      >  2:865264/1‑140 (MQ=255)      ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGt                                                                                                                                 >  2:60285/1‑140 (MQ=255)             cccTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTc                                                                                                                          <  2:1105431/140‑1 (MQ=255)                tttCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTg                                                                                                                       >  2:323291/1‑140 (MQ=255)                 ttCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGc                                                                                                                      >  1:939019/1‑140 (MQ=255)                  tCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCa                                                                                                                     >  2:883441/1‑140 (MQ=255)                  tCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCa                                                                                                                     >  2:496734/1‑140 (MQ=255)                       gCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAata                                                                                                                >  1:186668/1‑140 (MQ=255)                       gCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAata                                                                                                                <  2:939019/140‑1 (MQ=255)                       gCGATGGCTGGGACGGTCCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAata                                                                                                                <  1:146000/140‑1 (MQ=255)                        cGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAatat                                                                                                               >  1:240082/1‑140 (MQ=255)                                       ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtct                                                                                                >  2:251517/1‑140 (MQ=255)                                       ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtct                                                                                                >  2:37670/1‑140 (MQ=255)                                        tCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtctc                                                                                               <  1:251517/140‑1 (MQ=255)                                        tCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGt                                                                                                  <  1:998044/137‑1 (MQ=255)                                        tCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGt                                                                                                  >  2:998044/1‑137 (MQ=255)                                            aCCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCa                                                                                           >  2:418539/1‑140 (MQ=255)                                                 gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCAcc                                                                                                    >  2:912601/1‑126 (MQ=255)                                                 gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCAcc                                                                                                    <  1:912601/126‑1 (MQ=255)                                                 gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAca                                                                                      >  2:235214/1‑140 (MQ=255)                                                       tatTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaatcaat                                                                                <  2:1135588/140‑1 (MQ=255)                                                            tcCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGt                                                                           <  1:1011001/140‑1 (MQ=255)                                                             ccAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGtt                                                                          <  1:235214/140‑1 (MQ=255)                                                                 cACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTTTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTc                                                                      >  1:622012/1‑140 (MQ=255)                                                                   ccGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTAATCCa                                                                    >  2:1131882/1‑140 (MQ=255)                                                                        tcCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAAc                                                               >  1:1056427/1‑140 (MQ=255)                                                                         ccATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACg                                                              <  2:240082/140‑1 (MQ=255)                                                                            tGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcag                                                           <  1:285404/140‑1 (MQ=255)                                                                            tGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcag                                                           <  1:94614/140‑1 (MQ=255)                                                                             gTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagc                                                          <  1:496734/140‑1 (MQ=255)                                                                              ttttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca                                                         <  1:412678/140‑1 (MQ=255)                                                                              ttttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca                                                         <  2:284159/140‑1 (MQ=255)                                                                                ttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACg                                                       >  1:911557/1‑140 (MQ=255)                                                                                             cTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCg                                          >  2:426868/1‑140 (MQ=255)                                                                                               aTTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGAc                                        >  2:252547/1‑140 (MQ=255)                                                                                                 tCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTc                                      <  1:37670/140‑1 (MQ=255)                                                                                                     cTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGc                                  >  1:252183/1‑140 (MQ=255)                                                                                                     cTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGc                                  >  2:1130123/1‑140 (MQ=255)                                                                                                                  cTCGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGcc                     <  2:252183/140‑1 (MQ=255)                                                                                                                    cGCTCTTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTg                   >  2:193545/1‑140 (MQ=255)                                                                                                                       tctTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGaa                <  1:865264/140‑1 (MQ=255)                                                                                                                        ctTTCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAAt               >  2:1130881/1‑140 (MQ=255)                                                                                                                           tCAGGGAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCAt            >  2:657173/1‑140 (MQ=255)                                                                                                                              gggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAgg         <  1:418539/140‑1 (MQ=255)                                                                                                                               ggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGc        <  1:883441/140‑1 (MQ=255)                                                                                                                               ggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGc        <  1:252547/140‑1 (MQ=255)                                                                                                                               ggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGc        <  1:426868/140‑1 (MQ=255)                                                                                                                               ggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGc        <  2:186668/140‑1 (MQ=255)                                                                                                                               ggAGAGGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGc        <  1:60285/140‑1 (MQ=255)                                                                                                                                   agGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCa       >  2:411713/1‑137 (MQ=255)                                                                                                                                   agGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCa       <  1:411713/137‑1 (MQ=255)                                                                                                                                   agGGTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCCTTTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCAACt    >  2:1094554/1‑140 (MQ=255)                                                                                                                                    gggTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCAACTg   <  2:1056427/140‑1 (MQ=255)                                                                                                                                     ggTGGCCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGGATCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAAGCAACTGc  >  1:851694/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGACTCAAGCCAGCTAGTGCGCCTGGAATCATAGGCAACTGC  >  NC_000913/1351285‑1351556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑