Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,502,450	A→G	intergenic (+295/‑7)	tehB → / → ydcL	tellurite methyltransferase/DUF3313 domain‑containing lipoprotein YdcL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,502,450	0	A	G	100.0%	199.2 / NA	55	intergenic (+295/‑7)	tehB/ydcL	tellurite methyltransferase/DUF3313 domain‑containing lipoprotein YdcL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

ACTTAGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAACTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTAAAAGA  >  NC_000913/1502313‑1502584                                                                                                                                          |                                                                                                                                       aCTTAGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCt                                                                                                                                      >  2:917440/1‑140 (MQ=255) aCTTAGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCt                                                                                                                                      >  2:422443/1‑140 (MQ=255)   ttAGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTAt                                                                                                                                    <  2:290630/140‑1 (MQ=255)     aGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTc                                                                                                                                  >  1:715614/1‑140 (MQ=255)     aGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTc                                                                                                                                  <  1:513204/140‑1 (MQ=255)       ccccTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCAt                                                                                                                                <  2:287445/140‑1 (MQ=255)        cccTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATg                                                                                                                               <  2:892167/140‑1 (MQ=255)         ccTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGc                                                                                                                              <  2:260664/140‑1 (MQ=255)           tttttCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGt                                                                                                                            >  2:559623/1‑140 (MQ=255)           tttttCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGt                                                                                                                            >  1:820330/1‑140 (MQ=255)           tttttCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGt                                                                                                                            >  2:416932/1‑140 (MQ=255)                        aCTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTg                                                                                                               >  2:215827/1‑140 (MQ=255)                          tATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCg                                                                                                             <  1:559623/140‑1 (MQ=255)                             gtcgtcTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGt                                                                                                                            >  2:704305/1‑122 (MQ=255)                             gtcgtcTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGt                                                                                                                            <  1:704305/122‑1 (MQ=255)                                gtcTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGtt                                                                                                       >  1:733885/1‑140 (MQ=255)                                  cTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGc                                                                                                     >  2:465189/1‑140 (MQ=255)                                  cTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGc                                                                                                     >  2:134616/1‑140 (MQ=255)                                  cTGAAAAGGGGCTGAAAAATTTATTTTCACCAACAC‑TTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCa                                                                                                    >  1:323373/1‑140 (MQ=255)                                   tGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCa                                                                                                    >  1:780153/1‑140 (MQ=255)                                           ggCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATg                                                                                            <  2:29163/140‑1 (MQ=255)                                            gCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGc                                                                                           >  2:882307/1‑140 (MQ=255)                                             cTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCg                                                                                          <  2:780153/140‑1 (MQ=255)                                              tGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCgg                                                                                         <  1:215827/140‑1 (MQ=255)                                                      ttatttTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTgg                                                                                 <  1:422443/140‑1 (MQ=255)                                                      ttatttTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTgg                                                                                 <  1:375109/140‑1 (MQ=255)                                                        atttTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGct                                                                               <  1:917440/140‑1 (MQ=255)                                                        atttTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGct                                                                               <  1:465189/140‑1 (MQ=255)                                                        atttTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGct                                                                               <  2:1149545/140‑1 (MQ=255)                                                         ttttCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGctc                                                                              <  2:715614/140‑1 (MQ=255)                                                                     cTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTtgtg                                                                  <  1:416932/140‑1 (MQ=255)                                                                        tttttGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTtgtg                                                                  >  2:610571/1‑137 (MQ=255)                                                                        tttttGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTtgtg                                                                  <  1:610571/137‑1 (MQ=255)                                                                                acaacaCGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATc                                                       >  1:1136521/1‑140 (MQ=255)                                                                                 caacaCGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCa                                                      <  2:733885/140‑1 (MQ=255)                                                                                       gAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACccagc                                                <  2:608171/140‑1 (MQ=255)                                                                                       gAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACccagc                                                <  2:917867/140‑1 (MQ=255)                                                                                       gAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACccagc                                                <  2:1136521/140‑1 (MQ=255)                                                                                       gAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACccagc                                                <  2:323373/140‑1 (MQ=255)                                                                                        aaGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACccagcc                                               >  1:362756/1‑140 (MQ=255)                                                                                              cgcTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATaa                                         >  2:485409/1‑140 (MQ=255)                                                                                              cgcTTTTTGCTATAACTTAGAAAGCAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATaa                                         >  2:262335/1‑140 (MQ=255)                                                                                               gcTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATaaa                                        >  1:1144690/1‑140 (MQ=255)                                                                                               gcTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATaaa                                        >  1:676178/1‑140 (MQ=255)                                                                                                      gCTATAACTTAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTg                                 <  2:362756/140‑1 (MQ=255)                                                                                                              ttAGAAAGTAATATAATCATCTCAGGAAGCTATTCATGCGTAACACATCATTTGCGAAAGTTGCAGCTTTATGCGCCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTa                         <  2:1082852/140‑1 (MQ=255)                                                                                                                      tAATATAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTa                 >  1:219020/1‑140 (MQ=255)                                                                                                                        atatAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTAct               >  2:688787/1‑140 (MQ=255)                                                                                                                        atatAATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTAct               <  1:882307/140‑1 (MQ=255)                                                                                                                         tataATCATCTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTActc              <  1:51953/140‑1 (MQ=255)                                                                                                                             atcatcTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGAt          <  2:529759/140‑1 (MQ=255)                                                                                                                                atcTCAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTa       >  1:101746/1‑140 (MQ=255)                                                                                                                                   tcAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTaaaa    >  1:724394/1‑140 (MQ=255)                                                                                                                                    cAGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTAAAAg   >  2:56392/1‑140 (MQ=255)                                                                                                                                     aGGAAGCTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTAAAAGa  >  1:929245/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ACTTAGCCCCTTTTTCGGCATTGACTATGTCGTCTGAAAAGGGGCTGAAAAATTTATTTTCACCAACACTTTTTTTGCCACAACACGAAGCGGCGCTTTTTGCTATAACTTAGAAAGTAATATAATCATCTCAGGAAACTATTCATGCGTACCACATCATTTGCGAAAGTTGCAGCTTTATGCGGCTTATTGGCTCTGTCTGGTTGTGCATCTAAAATCACCCAGCCAGATAAATATTCTGGTTTTTTAAACAATTACTCTGATTTAAAAGA  >  NC_000913/1502313‑1502584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑