Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,543,164	A→G	G299G (GGT→GGC)	narU ←	nitrate/nitrite transporter NarU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,543,164	0	A	G	100.0%	160.4 / NA	45	G299G (GGT→GGC)	narU	nitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

ACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAG  >  NC_000913/1543032‑1543292                                                                                                                                     |                                                                                                                                 aCCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATaa                                                                                                                           <  2:151642/140‑1 (MQ=255) aCCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATaa                                                                                                                           <  2:381289/140‑1 (MQ=255)  ccGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGATCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATaaa                                                                                                                          <  2:1163411/140‑1 (MQ=255)  ccGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATaaa                                                                                                                          <  2:477116/140‑1 (MQ=255)  ccGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATaaa                                                                                                                          <  1:370863/140‑1 (MQ=255)            tgCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAg                                                                                                                >  2:800083/1‑140 (MQ=255)                  gtaaggtaagGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGcc                                                                                                          >  1:526814/1‑140 (MQ=255)                      ggtaaggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGgc                                                                                                      <  1:1102904/140‑1 (MQ=255)                      ggtaaggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGgc                                                                                                      >  2:180234/1‑140 (MQ=255)                        taaggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGgcgc                                                                                                    <  1:800083/140‑1 (MQ=255)                        taaggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGgcgc                                                                                                    >  2:620404/1‑140 (MQ=255)                         aaggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCa                                                                                                   <  1:498327/140‑1 (MQ=255)                          aggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAg                                                                                                  >  1:662607/1‑140 (MQ=255)                           ggAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCAACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGa                                                                                                 <  2:662607/140‑1 (MQ=255)                             aaCAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAat                                                                                               >  1:99388/1‑140 (MQ=255)                             aaCAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAat                                                                                               <  2:377423/140‑1 (MQ=255)                                  cagGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTca                                                                                          >  2:622912/1‑140 (MQ=255)                                      gCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATc                                                                                      <  1:622912/140‑1 (MQ=255)                                       cACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATcc                                                                                     <  1:1103953/140‑1 (MQ=255)                                       cACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATcc                                                                                     >  1:863517/1‑140 (MQ=255)                                          tGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCggg                                                                                  >  1:311838/1‑140 (MQ=255)                                              aaTCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACt                                                                              <  1:146246/140‑1 (MQ=255)                                                      tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGttttt                                                                      <  2:498602/140‑1 (MQ=255)                                                      tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGttttt                                                                      >  1:884226/1‑140 (MQ=255)                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGc                                                                <  2:370703/140‑1 (MQ=255)                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGc                                                                <  1:29323/140‑1 (MQ=255)                                                                               ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGaaa                                             >  1:289091/1‑140 (MQ=255)                                                                                         ccgAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                   >  1:1067949/1‑140 (MQ=255)                                                                                               ttATCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAAcc                             <  1:371391/140‑1 (MQ=255)                                                                                                 aTCGGAAATAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGa                           >  2:615297/1‑140 (MQ=255)                                                                                                       aaTAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTgg                     <  2:99388/140‑1 (MQ=255)                                                                                                       aaTAGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTgg                     <  2:289091/140‑1 (MQ=255)                                                                                                          aGCACCACCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                  <  1:620404/140‑1 (MQ=255)                                                                                                            caccacCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAgg                >  1:753582/1‑140 (MQ=255)                                                                                                            caccacCAACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAgg                >  2:1132717/1‑140 (MQ=255)                                                                                                               caccaACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa             >  1:631247/1‑140 (MQ=255)                                                                                                                accaACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa            >  1:270293/1‑140 (MQ=255)                                                                                                                 ccaACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAg           >  1:784077/1‑140 (MQ=255)                                                                                                                  caACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGc          >  1:385552/1‑140 (MQ=255)                                                                                                                  caACCGACCGCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGc          <  1:1132717/140‑1 (MQ=255)                                                                                                                    accgaccgCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAg        <  2:863517/140‑1 (MQ=255)                                                                                                                        accgCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc    >  2:1033927/1‑140 (MQ=255)                                                                                                                         ccgCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca   <  2:270293/140‑1 (MQ=255)                                                                                                                         ccgCGCGATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca   <  1:1033927/140‑1 (MQ=255)                                                                                                                          cgcgcgATGGCGCCGATAAATGGGCCAAAGAACGCCAGGCGTAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGCTAATGCAGGCTcag  >  2:715532/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 ACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAG  >  NC_000913/1543032‑1543292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑