Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	151,130	A→G	V157A (GTA→GCA)	yadK ←	putative fimbrial protein YadK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	151,130	0	A	G	100.0%	361.6 / NA	99	V157A (GTA→GCA)	yadK	putative fimbrial protein YadK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/49);  total (50/49)

TGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTACTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATATTTGCCTA  >  NC_000913/151001‑151261                                                                                                                                  |                                                                                                                                    tgttATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAAc                                                                                                                           <  1:926161/140‑1 (MQ=255)   ttATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACgg                                                                                                                         >  2:701/1‑140 (MQ=255)   ttATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACgg                                                                                                                         >  2:786996/1‑140 (MQ=255)   ttATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACgg                                                                                                                         >  2:729088/1‑140 (MQ=255)     aTTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGaa                                                                                                                       >  1:688291/1‑140 (MQ=255)          tagtaAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACgaga                                                                                                                  <  1:315265/140‑1 (MQ=255)           agtaAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGAt                                                                                                                 >  2:591413/1‑140 (MQ=255)             taAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATcc                                                                                                               >  1:866536/1‑140 (MQ=255)             taAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATcc                                                                                                               >  1:1148206/1‑140 (MQ=255)                atatCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGcc                                                                                                            >  2:301261/1‑140 (MQ=255)                  atCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTg                                                                                                          <  1:76384/140‑1 (MQ=255)                    ccACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGaa                                                                                                        >  1:715637/1‑140 (MQ=255)                     cACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAg                                                                                                       >  1:513621/1‑140 (MQ=255)                     cACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAg                                                                                                       >  1:804748/1‑140 (MQ=255)                     cACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAg                                                                                                       >  2:413320/1‑140 (MQ=255)                      aCTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGc                                                                                                      <  1:1090331/140‑1 (MQ=255)                      aCTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGc                                                                                                      <  1:1153362/140‑1 (MQ=255)                        tAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCAt                                                                                                    >  1:884966/1‑140 (MQ=255)                         aaCACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCAtt                                                                                                   >  2:520112/1‑140 (MQ=255)                             caTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTa                                                                                                  >  2:859360/1‑137 (MQ=255)                             caTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTa                                                                                                  <  1:859360/137‑1 (MQ=255)                             caTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGa                                                                                               <  2:399445/140‑1 (MQ=255)                             caTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGa                                                                                               >  1:588871/1‑140 (MQ=255)                             caTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGa                                                                                               <  2:588871/140‑1 (MQ=255)                              aTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTa                                                                                                  <  1:670290/136‑1 (MQ=255)                              aTGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTa                                                                                                  >  2:670290/1‑136 (MQ=255)                               tGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAAc                                                                                             >  2:896259/1‑140 (MQ=255)                                ggCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGa                                                                                               >  1:183459/1‑137 (MQ=255)                                ggCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGa                                                                                               <  2:183459/137‑1 (MQ=255)                                ggCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACa                                                                                            <  1:591413/140‑1 (MQ=255)                                  cTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACAtt                                                                                          >  2:462342/1‑140 (MQ=255)                                  cTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACAtt                                                                                          >  1:39019/1‑140 (MQ=255)                                  cTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACAtt                                                                                          >  1:644813/1‑140 (MQ=255)                                      tAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGTATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCAt                                                                                                    <  2:1152029/126‑1 (MQ=255)                                      tAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGTATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCAt                                                                                                    >  1:1152029/1‑126 (MQ=255)                                          ttGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTAt                                                                                  <  2:669004/140‑1 (MQ=255)                                               cAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGcc                                                                             <  1:646555/140‑1 (MQ=255)                                               cAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGcc                                                                             <  2:215488/140‑1 (MQ=255)                                                  aTGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGaa                                                                          <  2:321988/140‑1 (MQ=255)                                                    gTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATc                                                                        >  2:1007040/1‑140 (MQ=255)                                                    gTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCACGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATc                                                                        <  2:928003/140‑1 (MQ=255)                                                      aaCACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCt                                                                      <  2:550371/140‑1 (MQ=255)                                                       acacTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAAAGTATTCGCCGAATCCTg                                                                     >  2:1035550/1‑140 (MQ=255)                                                        cacTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGa                                                                    <  2:715637/140‑1 (MQ=255)                                                         acTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAg                                                                   >  1:442580/1‑140 (MQ=255)                                                         acTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAg                                                                   >  2:155075/1‑140 (MQ=255)                                                         acTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAg                                                                   >  2:296484/1‑140 (MQ=255)                                                           tCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTa                                                                 <  2:39019/140‑1 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGcc                                                                             <  1:611747/127‑1 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGcc                                                                             >  2:611747/1‑127 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAg                                                                >  1:254200/1‑140 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAg                                                                >  1:255921/1‑140 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAg                                                                >  1:275128/1‑140 (MQ=255)                                                            cAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAg                                                                >  2:1147388/1‑140 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGTATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTa                                                                 >  1:111972/1‑138 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGTATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTa                                                                 <  2:111972/138‑1 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAGGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGa                                                               >  1:829123/1‑140 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGa                                                               <  2:866536/140‑1 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGa                                                               >  2:9658/1‑140 (MQ=255)                                                             aaTGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGa                                                               >  1:85433/1‑140 (MQ=255)                                                                gCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGaaaa                                                            <  1:296484/140‑1 (MQ=255)                                                                 cAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAGGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAat                                                           <  2:829123/140‑1 (MQ=255)                                                                   ggATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAatat                                                         >  1:1019969/1‑140 (MQ=255)                                                                    gATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTa                                                                 >  2:1066513/1‑131 (MQ=255)                                                                    gATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTa                                                                 <  1:1066513/131‑1 (MQ=255)                                                                    gATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAAtata                                                        >  2:897232/1‑140 (MQ=255)                                                                    gATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAAtata                                                        <  2:662238/140‑1 (MQ=255)                                                                     aTCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATaa                                                       >  2:1160467/1‑140 (MQ=255)                                                                     aTCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACCAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATaa                                                       <  1:94045/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  1:1069457/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  1:120247/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  1:786996/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  2:688291/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  2:884966/140‑1 (MQ=255)                                                                      tCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAac                                                      <  1:1054814/140‑1 (MQ=255)                                                                       cGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAact                                                     >  1:505297/1‑140 (MQ=255)                                                                       cGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCACCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAact                                                     >  1:324122/1‑140 (MQ=255)                                                                        gATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAacta                                                    <  2:512782/140‑1 (MQ=255)                                                                        gATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAacta                                                    <  1:1147388/140‑1 (MQ=255)                                                                         aTTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAactac                                                   <  2:644813/140‑1 (MQ=255)                                                                          ttttttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAactact                                                  <  2:773143/140‑1 (MQ=255)                                                                           tttttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTc                                                 <  1:823985/140‑1 (MQ=255)                                                                            ttttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTcc                                                <  2:95782/140‑1 (MQ=255)                                                                              ttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAactac                                                   <  1:311859/135‑1 (MQ=255)                                                                              ttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAactac                                                   >  2:311859/1‑135 (MQ=255)                                                                              ttGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTa                                              >  1:1127615/1‑140 (MQ=255)                                                                                           cTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTg                                     <  2:1128405/136‑1 (MQ=255)                                                                                           cTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTg                                     >  1:1128405/1‑136 (MQ=255)                                                                                                        aTGAACCATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCa                    <  1:1007040/140‑1 (MQ=255)                                                                                                             ccATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGc               <  1:413320/140‑1 (MQ=255)                                                                                                              cATTCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCa              <  2:1019969/140‑1 (MQ=255)                                                                                                                ttCATGGCCACCGACATTGCTGGGTAAACTGAACGAGAACCGCCTGAAACCTTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCaaa            <  2:1148206/140‑1 (MQ=255)                                                                                                                 tCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCa                    <  1:1092417/131‑1 (MQ=255)                                                                                                                 tCATGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCa                    >  2:1092417/1‑131 (MQ=255)                                                                                                                   aTGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAatat         <  2:442580/140‑1 (MQ=255)                                                                                                                    tGTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATAtt        <  2:370127/140‑1 (MQ=255)                                                                                                                     gTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATAttt       >  1:855710/1‑140 (MQ=255)                                                                                                                     gTCATCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATAttt       >  1:584318/1‑140 (MQ=255)                                                                                                                        aTCCGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATATTTGcc    >  2:260913/1‑140 (MQ=255)                                                                                                                          ccGACATTGCTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATATTTGCCTa  <  1:729088/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    TGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCATTCGGGTGCTAAATTTCCAGGATGAACCATTCATGTCATCCGACATTACTGGATAAACGGAACGAGATCCGCCTGAAGCATTAAGAACATTGAATGTATTCGCCGAATCCTGAGTAGAAAATATAACTACTCCTACATTACTTGCTCCTGACGAAATTTCATTAGCAAATATTTGCCTA  >  NC_000913/151001‑151261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑