Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,384	A→G	K448K (AAA→AAG)	dgcJ →	putative diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,384	0	A	G	100.0%	234.5 / NA	65	K448K (AAA→AAG)	dgcJ	putative diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

AGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAAC  >  NC_000913/1873255‑1873519                                                                                                                                  |                                                                                                                                        aGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAAc                                                                                                                               >  2:735468/1‑140 (MQ=255)   cTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACat                                                                                                                             >  1:613253/1‑140 (MQ=255)    tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACata                                                                                                                            >  1:691640/1‑140 (MQ=255)     cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACatat                                                                                                                           >  2:605093/1‑140 (MQ=255)     cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACatat                                                                                                                           >  1:396682/1‑140 (MQ=255)        gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                      >  2:1072372/1‑126 (MQ=255)        gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                      <  1:1072372/126‑1 (MQ=255)        gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc                                                                                                                        >  2:148068/1‑140 (MQ=255)        gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc                                                                                                                        >  1:315606/1‑140 (MQ=255)        gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc                                                                                                                        >  1:678588/1‑140 (MQ=255)            ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg                                                                                                                    <  2:476189/140‑1 (MQ=255)                    tCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAaga                                                                                                            >  2:321467/1‑140 (MQ=255)                     cGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAagag                                                                                                           >  1:428722/1‑140 (MQ=255)                       aTTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGAt                                                                                                         <  1:605093/140‑1 (MQ=255)                             aaaaGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt                                                                                                   <  1:427427/140‑1 (MQ=255)                                  tGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCtt                                                                                              >  1:690704/1‑140 (MQ=255)                                   gATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc                                                                                             <  1:735468/140‑1 (MQ=255)                                    aTTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt                                                                                                   >  2:292609/1‑133 (MQ=255)                                    aTTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt                                                                                                   <  1:292609/133‑1 (MQ=255)                                     ttATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCg                                                                                           <  2:690704/140‑1 (MQ=255)                                            aTCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTa                                                                                    <  2:351583/140‑1 (MQ=255)                                             tCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAc                                                                                   >  1:668665/1‑140 (MQ=255)                                             tCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAc                                                                                   <  1:419434/140‑1 (MQ=255)                                              ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                                  >  2:1104026/1‑140 (MQ=255)                                              ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                                  >  1:521040/1‑140 (MQ=255)                                              ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                                  <  1:148068/140‑1 (MQ=255)                                                     ggTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaa                                                                           >  2:119200/1‑140 (MQ=255)                                                       tGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaag                                                                         >  2:971219/1‑140 (MQ=255)                                                         gCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaagaa                                                                       >  2:189896/1‑140 (MQ=255)                                                         gCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaagaa                                                                       <  2:691640/140‑1 (MQ=255)                                                         gCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaagaa                                                                       >  1:770503/1‑140 (MQ=255)                                                          cGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaagaaa                                                                      <  1:1119118/140‑1 (MQ=255)                                                            aTGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAAc                                                                    >  1:13480/1‑140 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  2:315606/140‑1 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  1:794108/140‑1 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  1:12895/140‑1 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  2:613253/140‑1 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  2:823594/140‑1 (MQ=255)                                                             tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                   <  1:607300/140‑1 (MQ=255)                                                               aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt                                                                 <  2:304384/140‑1 (MQ=255)                                                               aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt                                                                 <  1:325941/140‑1 (MQ=255)                                                               aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt                                                                 <  1:1074789/140‑1 (MQ=255)                                                                      catcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTAc                                                          >  1:846284/1‑140 (MQ=255)                                                                         catTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatg                                                       >  2:983906/1‑140 (MQ=255)                                                                           ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                     <  2:396682/140‑1 (MQ=255)                                                                           ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                     >  2:533050/1‑140 (MQ=255)                                                                            tCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgatg                                                    <  1:983906/140‑1 (MQ=255)                                                                                gTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatga                                                      >  2:334094/1‑134 (MQ=255)                                                                                gTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatga                                                      <  1:334094/134‑1 (MQ=255)                                                                                gTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGta                                                >  1:805075/1‑140 (MQ=255)                                                                                      tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                          >  2:1007226/1‑140 (MQ=255)                                                                                      tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                          >  2:931937/1‑140 (MQ=255)                                                                                             cgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTCCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGAt                                   <  2:805075/140‑1 (MQ=255)                                                                                              gcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGGCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATg                                  <  1:186241/140‑1 (MQ=255)                                                                                              gcAAATTGCAGCACAACTGCATGAGCGAATCGAAAAGCGTCTGCAACATATCGCGCCCCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGCTACACGATGCGTATAAAGCATCCGagg                                  <  2:770503/140‑3 (MQ=255)                                                                                               cAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                                 <  1:971219/140‑1 (MQ=255)                                                                                                aaaTTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAg                                <  1:132532/140‑1 (MQ=255)                                                                                                         cacaACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTatat                       >  2:635510/1‑140 (MQ=255)                                                                                                              cTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCaa                  >  1:699899/1‑140 (MQ=255)                                                                                                              cTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCaa                  >  1:716120/1‑140 (MQ=255)                                                                                                              cTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCaa                  >  1:961826/1‑140 (MQ=255)                                                                                                                 ccTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATaa               >  2:806446/1‑140 (MQ=255)                                                                                                                  cTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg              <  1:189896/140‑1 (MQ=255)                                                                                                                  cTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg              <  2:846284/140‑1 (MQ=255)                                                                                                                   tGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGTGTTTATATGTCAATAAGc             <  2:954987/140‑1 (MQ=255)                                                                                                                           tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaaa     >  1:833217/1‑140 (MQ=255)                                                                                                                              aaaagCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAAc  >  2:911577/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        AGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAAC  >  NC_000913/1873255‑1873519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑