Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,966,676	A→G	V121A (GTA→GCA)	cheZ ←	chemotaxis protein CheZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,966,676	0	A	G	100.0%	177.2 / NA	51	V121A (GTA→GCA)	cheZ	chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

GCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGA  >  NC_000913/1966539‑1966812                                                                                                                                          |                                                                                                                                         gCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCa                                                                                                                                        >  2:489293/1‑140 (MQ=255)  cACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCAt                                                                                                                                       >  2:390612/1‑140 (MQ=255)            aaCTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGaaa                                                                                                                             <  2:251820/140‑1 (MQ=255)            aaCTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGaaa                                                                                                                             <  1:854053/140‑1 (MQ=255)             aCTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAAt                                                                                                                            >  1:58949/1‑140 (MQ=255)               tGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTg                                                                                                                          <  1:443572/140‑1 (MQ=255)                 gCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTg                                                                                                                          <  2:342687/138‑1 (MQ=255)                 gCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTg                                                                                                                          >  1:342687/1‑138 (MQ=255)                 gCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTc                                                                                                                        >  2:128321/1‑140 (MQ=255)                    ttCGATCTCCTGAATGACATCGATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCgtg                                                                                                                     <  2:387835/140‑1 (MQ=255)                    ttCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCgtg                                                                                                                     <  1:128321/140‑1 (MQ=255)                      cGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTa                                                                                                                   >  1:716609/1‑140 (MQ=255)                       gATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTAt                                                                                                                  <  1:489293/140‑1 (MQ=255)                         tctcCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCgtg                                                                                                                     >  2:1133688/1‑135 (MQ=255)                         tctcCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCgtg                                                                                                                     <  1:1133688/135‑1 (MQ=255)                         tctcCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCt                                                                                                                <  1:390612/140‑1 (MQ=255)                         tctcCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCt                                                                                                                <  1:964932/140‑1 (MQ=255)                              tGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTAc                                                                                                           <  2:607641/140‑1 (MQ=255)                              tGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTAc                                                                                                           <  2:1053422/140‑1 (MQ=255)                              tGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTAc                                                                                                           <  1:1082636/140‑1 (MQ=255)                                     catccatcATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCa                                                                                                    <  2:7455/140‑1 (MQ=255)                                     catccatcATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCa                                                                                                    >  2:480169/1‑140 (MQ=255)                                     catccatcATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCa                                                                                                    >  2:607847/1‑140 (MQ=255)                                            catcCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGt                                                                                             >  1:58924/1‑140 (MQ=255)                                              tcCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTAc                                                                                                           <  1:694928/124‑1 (MQ=255)                                              tcCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTAc                                                                                                           >  2:694928/1‑124 (MQ=255)                                                  cTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCaa                                                                                       >  1:1159051/1‑140 (MQ=255)                                                    tAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAgg                                                                                     >  1:220598/1‑140 (MQ=255)                                                      aTGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTc                                                                                   >  1:366657/1‑140 (MQ=255)                                                       tGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTCTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCa                                                                                  >  2:564864/1‑140 (MQ=255)                                                         aCCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTCTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAat                                                                                <  1:564864/140‑1 (MQ=255)                                                             gCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAatcgga                                                                            >  1:87838/1‑140 (MQ=255)                                                                     agaTCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCaaa                                                                    <  1:671024/140‑1 (MQ=255)                                                                        tCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCa                                                                 >  1:512104/1‑140 (MQ=255)                                                                          cTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGt                                                               >  1:964896/1‑140 (MQ=255)                                                                               aaTCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCa                                                                      <  2:378740/128‑1 (MQ=255)                                                                               aaTCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCa                                                                      >  1:378740/1‑128 (MQ=255)                                                                                aTCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATccc                                                         <  1:480169/140‑1 (MQ=255)                                                                                     gcgcCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATGGGCAAACCAGTCATCCCAACGt                                                    >  1:822321/1‑140 (MQ=255)                                                                                           tcatGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAGCGTTGGGtt                                              >  2:364535/1‑140 (MQ=255)                                                                                                 tttCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCt                                        >  1:315041/1‑140 (MQ=255)                                                                                                  ttCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCtt                                       <  1:607847/140‑1 (MQ=255)                                                                                                          gTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGAt                               <  2:716609/140‑1 (MQ=255)                                                                                                             gcgcGTTAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTtc                            >  2:110389/1‑140 (MQ=255)                                                                                                                  ttAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGAt                               >  2:924591/1‑132 (MQ=255)                                                                                                                  ttAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGAt                               <  1:924591/132‑1 (MQ=255)                                                                                                                   tAGTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCAtt                      >  1:798084/1‑140 (MQ=255)                                                                                                                     gTAAAGCTGGTATGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTg                    <  2:964896/140‑1 (MQ=255)                                                                                                                                aTGCGCGGGTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCg         >  1:914578/1‑140 (MQ=255)                                                                                                                                       ggTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGa  <  2:512104/140‑1 (MQ=255)                                                                                                                                       ggTGCATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGa  <  2:1159051/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGA  >  NC_000913/1966539‑1966812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑