Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,646	T→C	H292R (CAC→CGC)	cpsB ←	mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,646	0	T	C	100.0%	198.4 / NA	58	H292R (CAC→CGC)	cpsB	mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/29);  total (29/29)

ACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGC  >  NC_000913/2123511‑2123779                                                                                                                                        |                                                                                                                                      aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGgc                                                                                                                                   >  2:448600/1‑140 (MQ=255)   cGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGgcgc                                                                                                                                 >  2:562552/1‑140 (MQ=255)   cGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGgcgc                                                                                                                                 <  1:731493/140‑1 (MQ=255)     cATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTg                                                                                                                               <  1:749063/140‑1 (MQ=255)     cATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTg                                                                                                                               >  1:633582/1‑140 (MQ=255)     cATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTg                                                                                                                               <  1:341612/140‑1 (MQ=255)       tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAGACGTTGCCCTCGGCGGTGCGGGCGCTGAt                                                                                                                             <  1:1128383/140‑1 (MQ=255)       tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGAt                                                                                                                             <  1:803469/140‑1 (MQ=255)        cTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGAtc                                                                                                                            <  2:548687/140‑1 (MQ=255)                tGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTccc                                                                                                                        <  2:441036/136‑1 (MQ=255)                tGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTccc                                                                                                                        >  1:441036/1‑136 (MQ=255)                 gCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAAt                                                                                                                   <  1:448600/140‑1 (MQ=255)                        tACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGAcc                                                                                                            >  1:768961/1‑140 (MQ=255)                         aCCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCa                                                                                                           <  2:802784/140‑1 (MQ=255)                          ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAg                                                                                                          >  2:854381/1‑140 (MQ=255)                            aGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGACAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGa                                                                                                        >  2:100265/1‑140 (MQ=255)                               tCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGcc                                                                                                     >  1:377190/1‑140 (MQ=255)                                   tCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACa                                                                                                 <  1:731504/140‑1 (MQ=255)                                        ccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCt                                                                                            <  2:768961/140‑1 (MQ=255)                                           aCGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAg                                                                                                       <  1:338408/126‑1 (MQ=255)                                           aCGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAg                                                                                                       >  2:338408/1‑126 (MQ=255)                                                 gtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGc                                                                                   >  1:269152/1‑140 (MQ=255)                                                   gACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcat                                                                                 <  2:633582/140‑1 (MQ=255)                                                        ggCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                            <  1:327278/140‑1 (MQ=255)                                                              aTTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                            <  2:338097/134‑1 (MQ=255)                                                              aTTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                            >  1:338097/1‑134 (MQ=255)                                                                tCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGcaccac                                                                    >  2:889806/1‑140 (MQ=255)                                                                       acacATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCa                                                             >  1:422130/1‑140 (MQ=255)                                                                        cacaTAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                            <  2:381913/124‑1 (MQ=255)                                                                        cacaTAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                            >  1:381913/1‑124 (MQ=255)                                                                        cacaTAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGcac                                                                       <  2:653830/129‑1 (MQ=255)                                                                        cacaTAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGcac                                                                       >  1:653830/1‑129 (MQ=255)                                                                             aGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaac                                                                 <  2:784098/130‑1 (MQ=255)                                                                             aGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaac                                                                 >  1:784098/1‑130 (MQ=255)                                                                                  ttttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACg                                                  >  1:1119572/1‑140 (MQ=255)                                                                                  ttttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCACCCATCGGCACCACAACAGCATCTGCCGTACg                                                  >  1:268319/1‑140 (MQ=255)                                                                                     tCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTc                                               >  1:213321/1‑140 (MQ=255)                                                                                         ttttGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATg                                           <  2:1119572/140‑1 (MQ=255)                                                                                          tttGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGa                                          <  1:974564/140‑1 (MQ=255)                                                                                              tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCg                                                      >  2:930401/1‑124 (MQ=255)                                                                                              tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCg                                                      <  1:930401/124‑1 (MQ=255)                                                                                              tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACcgc                                      >  2:590808/1‑140 (MQ=255)                                                                                                  tAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTaa                                  <  1:708057/140‑1 (MQ=255)                                                                                                           cGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAATACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTTCATGACCGCGTAATCCACCGAc                         >  1:1091837/1‑140 (MQ=255)                                                                                                           cGCCGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGAc                         >  1:762524/1‑140 (MQ=255)                                                                                                              cGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGActct                      >  2:207659/1‑140 (MQ=255)                                                                                                              cGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGActct                      >  1:865472/1‑140 (MQ=255)                                                                                                              cGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGActct                      >  2:68479/1‑140 (MQ=255)                                                                                                              cGTGGCAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGActct                      >  1:838738/1‑140 (MQ=255)                                                                                                                   cAAACGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCgg                 >  1:761329/1‑140 (MQ=255)                                                                                                                    aaaCGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCggg                <  2:213321/140‑1 (MQ=255)                                                                                                                    aaaCGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCggg                <  2:838738/140‑1 (MQ=255)                                                                                                                       cGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAg             <  2:865472/140‑1 (MQ=255)                                                                                                                       cGTTGCCCTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCACCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAg             <  2:268319/140‑1 (MQ=255)                                                                                                                            cccTCGGCGGTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCgag        <  1:854381/140‑1 (MQ=255)                                                                                                                                 ggcggTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACg   >  2:905380/1‑140 (MQ=255)                                                                                                                                 ggcggTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACg   <  2:377190/140‑1 (MQ=255)                                                                                                                                  gcggTGCGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGc  <  2:554393/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGC  >  NC_000913/2123511‑2123779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑