Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,157,427	T→C	V722A (GTT→GCT)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,157,427	0	T	C	100.0%	277.7 / NA	76	V722A (GTT→GCT)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/46);  total (30/46)

TTTACCTTGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGTTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCGTGGTGCTGGAGCACA  >  NC_000913/2157291‑2157561                                                                                                                                         |                                                                                                                                       tttACCTTGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACATAGGGCTGGTGGCGTATGTCAATGCTGa                                                                                                                                     >  2:700490/1‑140 (MQ=255)   tACCTTGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATc                                                                                                                                   >  1:628174/1‑140 (MQ=255)     ccTTGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATcgc                                                                                                                                 >  2:121305/1‑140 (MQ=255)       ttGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGa                                                                                                                               >  1:709174/1‑140 (MQ=255)         gCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGa                                                                                                                               >  1:97057/1‑138 (MQ=255)         gCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGa                                                                                                                               <  2:97057/138‑1 (MQ=255)          cAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAGCTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAg                                                                                                                            >  1:708321/1‑140 (MQ=255)           aGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAgc                                                                                                                           >  2:834273/1‑140 (MQ=255)           aGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAgc                                                                                                                           >  2:86145/1‑140 (MQ=255)           aGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAgc                                                                                                                           >  2:100330/1‑140 (MQ=255)               cACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCa                                                                                                                       >  2:298433/1‑140 (MQ=255)                aCGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAg                                                                                                                      <  1:244028/140‑1 (MQ=255)                aCGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAg                                                                                                                      <  2:981926/140‑1 (MQ=255)                  gTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGcc                                                                                                                    <  2:709174/140‑1 (MQ=255)                    cACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGt                                                                                                                  <  2:446299/140‑1 (MQ=255)                     aCTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTc                                                                                                                 <  2:923207/140‑1 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAgcgc                                                                                                                         >  2:689322/1‑130 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAgcgc                                                                                                                         <  1:689322/130‑1 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTg                                                                                                               <  1:49931/140‑1 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTg                                                                                                               >  2:102855/1‑140 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTg                                                                                                               >  1:3841/1‑140 (MQ=255)                       tGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTg                                                                                                               <  1:364582/140‑1 (MQ=255)                           tGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGa                                                                                                           >  1:152894/1‑140 (MQ=255)                           tGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGa                                                                                                           >  1:975894/1‑140 (MQ=255)                            gcgcTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGAt                                                                                                          <  1:102855/140‑1 (MQ=255)                            gcgcTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGAt                                                                                                          <  1:379106/140‑1 (MQ=255)                                 cAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCa                                                                                                     >  1:952215/1‑140 (MQ=255)                                   gTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATg                                                                                                   >  1:1108368/1‑140 (MQ=255)                                   gTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATg                                                                                                   >  1:1032544/1‑140 (MQ=255)                                    tACCTGGGTGCCACAGTTGATGGAAGAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATgg                                                                                                  <  1:17069/140‑1 (MQ=255)                                          ggTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATg                                                                                            <  1:121305/140‑1 (MQ=255)                                           gTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGt                                                                                           <  1:183552/140‑1 (MQ=255)                                            tGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTc                                                                                          >  2:814740/1‑140 (MQ=255)                                              ccACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGa                                                                                        >  1:714441/1‑140 (MQ=255)                                                acaGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGGTCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATa                                                                                      <  1:527854/140‑1 (MQ=255)                                                acaGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATa                                                                                      <  1:298433/140‑1 (MQ=255)                                                acaGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATa                                                                                      <  2:975894/140‑1 (MQ=255)                                                acaGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATa                                                                                      <  1:814740/140‑1 (MQ=255)                                                  aGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAAc                                                                                    <  2:152894/140‑1 (MQ=255)                                                         ggAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGt                                                                             >  1:1104397/1‑140 (MQ=255)                                                              aaCTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAAc                                                                        >  1:995347/1‑140 (MQ=255)                                                                                  cTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGAt                                                    <  2:419249/140‑1 (MQ=255)                                                                                    ttCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGAttt                                                  <  1:834273/140‑1 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                <  2:883907/140‑1 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                >  2:968530/1‑140 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                <  2:995347/140‑1 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                >  1:508733/1‑140 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                <  1:658647/140‑1 (MQ=255)                                                                                      cTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTcc                                                <  1:763256/140‑1 (MQ=255)                                                                                        gATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCAc                                              <  2:708321/140‑1 (MQ=255)                                                                                        gATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCAc                                              <  1:100330/140‑1 (MQ=255)                                                                                        gATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCAc                                              <  1:858408/140‑1 (MQ=255)                                                                                                cAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTAGTCAGCGGCTGATTTCCACTATTtata                                      <  2:1032544/140‑1 (MQ=255)                                                                                                   cGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCAc                                              >  1:246326/1‑129 (MQ=255)                                                                                                   cGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCAc                                              <  2:246326/129‑1 (MQ=255)                                                                                                    gACTGGCAGGACAACGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCa                                  <  2:714441/140‑1 (MQ=255)                                                                                                      cTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAgg                                <  2:3841/140‑1 (MQ=255)                                                                                                      cTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAgg                                <  1:183178/140‑1 (MQ=255)                                                                                                      cTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAgg                                <  2:1094353/140‑1 (MQ=255)                                                                                                      cTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAgg                                <  2:1104397/140‑1 (MQ=255)                                                                                                       tGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGc                               >  2:609595/1‑140 (MQ=255)                                                                                                        ggCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGcc                              >  2:56355/1‑140 (MQ=255)                                                                                                        ggCAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGcc                              >  1:276727/1‑140 (MQ=255)                                                                                                          cAGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCaa                            <  1:737490/140‑1 (MQ=255)                                                                                                           aGGACAAAGGGCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAAc                           <  2:325033/140‑1 (MQ=255)                                                                                                                   gggCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATAc                                     >  1:986706/1‑122 (MQ=255)                                                                                                                   gggCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATAc                                     <  2:986706/122‑1 (MQ=255)                                                                                                                     gCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATcgcg                 <  2:369070/140‑1 (MQ=255)                                                                                                                     gCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATcgcg                 <  1:593841/140‑1 (MQ=255)                                                                                                                     gCTGGTGGCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATcgcg                 <  1:205259/140‑1 (MQ=255)                                                                                                                       tggtggCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCgtg               >  2:128231/1‑140 (MQ=255)                                                                                                                        ggtggCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCgtgg              <  2:1087296/140‑1 (MQ=255)                                                                                                                         gtggCGTATGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCgtggt             <  1:128231/140‑1 (MQ=255)                                                                                                                                aTGTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCGTGGTGCTGGAg      <  1:968530/140‑1 (MQ=255)                                                                                                                                  gTCAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTCCCACTATTTATACTCAGGCCAACCAGTATCGCGTGGTGCTGGAGca    <  2:952215/140‑1 (MQ=255)                                                                                                                                    cAATGCTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCGTGGTGCTGGAGcaca  <  2:508733/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TTTACCTTGCAGGCCACGTCACTGGATGCGCTCAGTACCTGGGTGCCACAGTTGATGGAAAAACTCCAGCAACTGCCACAGCTTTCTGATGTCTCCAGCGACTGGCAGGACAAAGGGCTGGTGGCGTATGTCAATGTTGATCGCGACAGCGCCAGCCGTCTGGGGATCAGCATGGCGGATGTCGATAACGCCCTGTACAACGCGTTTGGTCAGCGGCTGATTTCCACTATTTATACTCAGGCCAACCAGTATCGCGTGGTGCTGGAGCACA  >  NC_000913/2157291‑2157561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑