Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,174,447	C→T	R40R (CGG→CGA)	gatB ←	galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,174,447	0	C	T	100.0%	260.7 / NA	72	R40R (CGG→CGA)	gatB	galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/38);  total (34/38)

TTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTAT  >  NC_000913/2174311‑2174582                                                                                                                                         |                                                                                                                                        tttGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGa                                                                                                                                      <  1:92812/140‑1 (MQ=255)   tGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGaca                                                                                                                                    >  1:183470/1‑140 (MQ=255)    gTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGacac                                                                                                                                   <  2:519066/140‑1 (MQ=255)    gTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGacac                                                                                                                                   <  1:399339/140‑1 (MQ=255)        tGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGa                                                                                                                               >  2:389375/1‑140 (MQ=255)          cTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGAtt                                                                                                                             >  1:890620/1‑140 (MQ=255)             cgataccgatacCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAAt                                                                                                                          >  1:616763/1‑140 (MQ=255)                 accgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCaa                                                                                                                      <  2:890620/140‑1 (MQ=255)                   cgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACa                                                                                                                    <  2:1118236/140‑1 (MQ=255)                   cgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACa                                                                                                                    >  2:121152/1‑140 (MQ=255)                     ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGaca                                                                                                                                    <  1:454058/122‑1 (MQ=255)                     ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGaca                                                                                                                                    >  2:454058/1‑122 (MQ=255)                     ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAgg                                                                                                                  <  1:580075/140‑1 (MQ=255)                     ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAgg                                                                                                                  >  2:518252/1‑140 (MQ=255)                     ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAgg                                                                                                                  >  2:608930/1‑140 (MQ=255)                      taccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGa                                                                                                                 >  2:765461/1‑140 (MQ=255)                      taccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGa                                                                                                                 <  1:567180/140‑1 (MQ=255)                       accAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGaa                                                                                                                <  1:515994/140‑1 (MQ=255)                       accAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGaa                                                                                                                <  2:438045/140‑1 (MQ=255)                           gAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAAtatt                                                                                                            <  1:652205/140‑1 (MQ=255)                           gAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAAtatt                                                                                                            <  1:608930/140‑1 (MQ=255)                               tAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGa                                                                                                        <  2:107089/140‑1 (MQ=255)                                aaaaGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGAt                                                                                                       <  1:3472/140‑1 (MQ=255)                                  aaGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTc                                                                                                     <  1:836165/140‑1 (MQ=255)                                  aaGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTc                                                                                                     <  1:758530/140‑1 (MQ=255)                                        tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGacac                                                                                               >  1:34707/1‑140 (MQ=255)                                        tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGacac                                                                                               <  1:518252/140‑1 (MQ=255)                                         gCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGAcaca                                                                                              >  2:115718/1‑140 (MQ=255)                                          ccGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACaa                                                                                             <  2:445812/140‑1 (MQ=255)                                           cGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAAc                                                                                            <  1:511409/140‑1 (MQ=255)                                                            tCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgc                                                                           <  1:1112275/140‑1 (MQ=255)                                                              gCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgccg                                                                         <  1:765461/140‑1 (MQ=255)                                                              gCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgcc                                                                          <  1:63012/139‑1 (MQ=255)                                                              gCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgcc                                                                          >  2:63012/1‑139 (MQ=255)                                                                      tACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTa                                                                 >  2:181774/1‑140 (MQ=255)                                                                      tACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTa                                                                 <  2:1121884/140‑1 (MQ=255)                                                                         gATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTagag                                                              >  1:620727/1‑140 (MQ=255)                                                                                tttGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCaa                                                       >  2:680507/1‑140 (MQ=255)                                                                                 ttGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAAc                                                      <  2:34707/140‑1 (MQ=255)                                                                                  tGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAAcc                                                     <  1:121152/140‑1 (MQ=255)                                                                                     cAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACcgcg                                                  >  1:797493/1‑140 (MQ=255)                                                                                          gtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTcc                                             >  1:291214/1‑140 (MQ=255)                                                                                                atCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGc                                       <  2:291214/140‑1 (MQ=255)                                                                                                  cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCaa                                         >  2:975148/1‑136 (MQ=255)                                                                                                  cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCaa                                         <  1:975148/136‑1 (MQ=255)                                                                                                  cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGa                                     <  1:181774/140‑1 (MQ=255)                                                                                                    aaTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACa                                   >  1:1030743/1‑140 (MQ=255)                                                                                                    aaTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACa                                   >  2:753174/1‑140 (MQ=255)                                                                                                     aTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACaa                                  >  1:1047371/1‑140 (MQ=255)                                                                                                     aTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACaa                                  >  1:700085/1‑140 (MQ=255)                                                                                                     aTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACaa                                  >  1:633346/1‑140 (MQ=255)                                                                                                     aTGCACACCATCCATATAGGTTTCTATTTCATTAACTCGACACAGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGGGCCTCCGCAAGCGACaa                                  >  2:1036657/1‑140 (MQ=255)                                                                                                             catccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTg                          >  2:517807/1‑140 (MQ=255)                                                                                                             catccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTg                          >  1:123310/1‑140 (MQ=255)                                                                                                              atccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGc                         <  1:1162337/140‑1 (MQ=255)                                                                                                               tccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCg                        >  1:446254/1‑140 (MQ=255)                                                                                                                ccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGt                       <  1:680507/140‑1 (MQ=255)                                                                                                                ccatATAGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGt                       <  1:389375/140‑1 (MQ=255)                                                                                                                   tataGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTc                    <  1:753174/140‑1 (MQ=255)                                                                                                                     taGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCAt                  >  2:933327/1‑140 (MQ=255)                                                                                                                      aGGTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATa                 >  1:863519/1‑140 (MQ=255)                                                                                                                       ggTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATaa                >  1:984516/1‑140 (MQ=255)                                                                                                                        gTTTCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATaaa               <  1:517807/140‑1 (MQ=255)                                                                                                                          ttCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATa             <  2:797493/140‑1 (MQ=255)                                                                                                                          ttCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATa             >  2:919212/1‑140 (MQ=255)                                                                                                                           tCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAg            >  1:214680/1‑140 (MQ=255)                                                                                                                           tCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAg            >  1:239551/1‑140 (MQ=255)                                                                                                                           tCTATTTCATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAg            >  1:604536/1‑140 (MQ=255)                                                                                                                                  cATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCtttt     <  2:700085/140‑1 (MQ=255)                                                                                                                                  cATTAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCtttt     <  2:446254/140‑1 (MQ=255)                                                                                                                                    ttAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTa   <  2:301306/140‑1 (MQ=255)                                                                                                                                     tAACTCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTAt  <  2:239551/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTAT  >  NC_000913/2174311‑2174582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑