Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,237,776 T→C H505R (CAC→CGC)  mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,237,7760TC100.0% 197.5 / NA 55H505R (CAC→CGC) mglAD‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

ACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATA  >  NC_000913/2237640‑2237910
                                                                                                                                        |                                                                                                                                      
aCTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCa                                                                                                                                     >  1:900546/1‑140 (MQ=255)
 cTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCaa                                                                                                                                    <  1:3033/140‑1 (MQ=255)
      aaaTGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacg                                                                                                                               <  2:955953/140‑1 (MQ=255)
             gggTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaagacg                                                                                                                        <  1:990087/140‑1 (MQ=255)
                   tGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGGAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAt                                                                                                                  >  1:589198/1‑140 (MQ=255)
                   tGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAt                                                                                                                  >  2:219140/1‑140 (MQ=255)
                             taatCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGc                                                                                                        <  1:625900/140‑1 (MQ=255)
                                  gCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                                                                   <  2:397769/140‑1 (MQ=255)
                                             tAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAt                                                                                        <  2:924084/140‑1 (MQ=255)
                                              aaaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATc                                                                                       >  2:744181/1‑140 (MQ=255)
                                                 aGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGAc                                                                                    <  2:219821/140‑1 (MQ=255)
                                                       acgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTcc                                                                              >  2:774110/1‑140 (MQ=255)
                                                       acgTAAATACCGCCCTCTTTCAGATAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTcc                                                                              >  2:13532/1‑140 (MQ=255)
                                                                  gCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAc                                                                   >  1:1111764/1‑140 (MQ=255)
                                                                   cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAcc                                                                  >  2:232624/1‑140 (MQ=255)
                                                                   cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAcc                                                                  >  1:533039/1‑140 (MQ=255)
                                                                    ccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                                                                 <  1:527528/140‑1 (MQ=255)
                                                                     ctctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAt                                                                <  1:744181/140‑1 (MQ=255)
                                                                        tttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGc                                                             >  1:163444/1‑140 (MQ=255)
                                                                         ttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCt                                                            <  2:900546/140‑1 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:597479/1‑140 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:551230/1‑140 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:315679/1‑140 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:458072/1‑140 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:496456/1‑140 (MQ=255)
                                                                                  gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                                   >  2:1067538/1‑140 (MQ=255)
                                                                                   taagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGa                                                  >  2:629719/1‑140 (MQ=255)
                                                                                     agAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt                                                <  2:1111764/140‑1 (MQ=255)
                                                                                     agAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt                                                <  2:163444/140‑1 (MQ=255)
                                                                                         aaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACgg                                            <  1:635249/140‑1 (MQ=255)
                                                                                         aaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACgg                                            <  2:533039/140‑1 (MQ=255)
                                                                                         aaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACgg                                            <  1:629719/140‑1 (MQ=255)
                                                                                           cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTc                                          >  1:132342/1‑140 (MQ=255)
                                                                                                  tATTTAACGCACTCATGGGAAGCCCCTAATCTTTAAGGCGCAAAGACGCAAGACGCAGAATTTCATTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATc                                   <  2:928084/140‑1 (MQ=255)
                                                                                                   aTTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATcc                                  >  1:200782/1‑140 (MQ=255)
                                                                                                         cGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTaaca                            >  1:970625/1‑140 (MQ=255)
                                                                                                                aTGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg                     <  1:551230/140‑1 (MQ=255)
                                                                                                                     aaGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCAttt                <  1:1072412/140‑1 (MQ=255)
                                                                                                                       gCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCa              <  1:414453/140‑1 (MQ=255)
                                                                                                                        ccccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCag             >  1:1019056/1‑140 (MQ=255)
                                                                                                                        ccccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCag             >  1:968125/1‑140 (MQ=255)
                                                                                                                        ccccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCag             >  1:827281/1‑140 (MQ=255)
                                                                                                                         cccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCaga            <  2:676534/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           <  1:774110/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           <  2:64046/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           <  2:132342/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           <  2:589198/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           <  2:874178/140‑1 (MQ=255)
                                                                                                                          ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCagag           >  1:992038/1‑140 (MQ=255)
                                                                                                                            tAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAgagga         <  2:827281/140‑1 (MQ=255)
                                                                                                                              aTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGgaga       <  1:496456/140‑1 (MQ=255)
                                                                                                                               tCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGat      <  1:458072/140‑1 (MQ=255)
                                                                                                                               tCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGat      <  1:315679/140‑1 (MQ=255)
                                                                                                                               tCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGat      <  1:172749/140‑1 (MQ=255)
                                                                                                                                   aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGataata  >  1:255878/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATA  >  NC_000913/2237640‑2237910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑