Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,305,395	C→T	G454R (GGG→AGG)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,305,395	0	C	T	100.0%	139.6 / NA	43	G454R (GGG→AGG)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

GTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTT  >  NC_000913/2305258‑2305530                                                                                                                                          |                                                                                                                                        gTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTCCGCGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGGTGCCCTCa                                                                                                                                       >  2:817316/1‑140 (MQ=255)    cAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGa                                                                                                                                    >  2:788481/1‑140 (MQ=255)      gCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAgc                                                                                                                                  <  2:21662/140‑1 (MQ=255)          gCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCgg                                                                                                                              >  2:289316/1‑140 (MQ=255)          gCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCgg                                                                                                                              >  2:803206/1‑140 (MQ=255)                    gACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTcc                                                                                                                    <  2:334756/140‑1 (MQ=255)                              tCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCa                                                                                                          >  1:105590/1‑140 (MQ=255)                              tCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCa                                                                                                          <  1:134364/140‑1 (MQ=255)                              tCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCa                                                                                                          <  1:733790/140‑1 (MQ=255)                                gCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACt                                                                                                        >  2:35506/1‑140 (MQ=255)                                   ttCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgac                                                                                                     <  1:1124480/140‑1 (MQ=255)                                   ttCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgac                                                                                                     >  2:453616/1‑140 (MQ=255)                                   ttCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgac                                                                                                     <  1:509056/140‑1 (MQ=255)                                    tCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgacg                                                                                                    <  2:31261/140‑1 (MQ=255)                                        cGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACtt                                                                                                <  1:788481/140‑1 (MQ=255)                                            gCCTGCCATTGCGGGCTGGAAACATGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTgg                                                                                                             <  2:1055387/123‑1 (MQ=255)                                            gCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTgg                                                                                                             >  1:1055387/1‑123 (MQ=255)                                            gCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGt                                                                                            >  1:1119265/1‑140 (MQ=255)                                                gCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTAccc                                                                                        >  1:1061756/1‑140 (MQ=255)                                                gCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTAccc                                                                                        >  1:178586/1‑140 (MQ=255)                                                gCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTAccc                                                                                        >  2:132737/1‑140 (MQ=255)                                                gCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCCGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTTCTGGTTACTGACGACTTCAGTAccc                                                                                        >  2:200411/1‑140 (MQ=255)                                                     tGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACg                                                                                   <  1:132737/140‑1 (MQ=255)                                                       cGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCTCTGACGACTTCAGTACCCAGACGCa                                                                                 <  1:200411/140‑1 (MQ=255)                                                         ggCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGt                                                                               >  2:140805/1‑140 (MQ=255)                                                              gAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGcc                                                                          >  1:159235/1‑140 (MQ=255)                                                               aaaCACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCa                                                                         <  2:105590/140‑1 (MQ=255)                                                               aaaCACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCa                                                                         <  1:289316/140‑1 (MQ=255)                                                                     gATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTtc                                                                   >  1:843720/1‑140 (MQ=255)                                                                           cAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCa                                                             <  1:803206/140‑1 (MQ=255)                                                                            aaaTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCAt                                                            <  2:178586/140‑1 (MQ=255)                                                                            aaaTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCAt                                                            <  1:140805/140‑1 (MQ=255)                                                                              aTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCa                                                          >  1:881630/1‑140 (MQ=255)                                                                               tACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCAc                                                         <  1:35506/140‑1 (MQ=255)                                                                                 cTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGc                                                       <  2:159235/140‑1 (MQ=255)                                                                                     ttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGa                                                   >  2:260198/1‑140 (MQ=255)                                                                                         agcagATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAAt                                               >  1:265107/1‑140 (MQ=255)                                                                                                         tCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcccc                               >  1:310882/1‑140 (MQ=255)                                                                                                            gcgcggcgGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCt                            >  2:419403/1‑140 (MQ=255)                                                                                                              gcggcgGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCAcg                                        <  2:867982/126‑1 (MQ=255)                                                                                                              gcggcgGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCAcg                                        >  1:867982/1‑126 (MQ=255)                                                                                                              gcggcgGTTGACGCCCCTGGCGATGCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTg                          >  1:19983/1‑140 (MQ=255)                                                                                                                                      gCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt  >  2:143048/1‑140 (MQ=255)                                                                                                                                      gCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt  >  2:683647/1‑140 (MQ=255)                                                                                                                                      gCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt  <  1:551678/140‑1 (MQ=255)                                                                                                                                      gCCCTCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt  <  1:419403/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTT  >  NC_000913/2305258‑2305530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑