Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,553	T→C	T94A (ACG→GCG)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,553	0	T	C	100.0%	267.6 / NA	75	T94A (ACG→GCG)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/32);  total (43/32)

CTGCATTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAG  >  NC_000913/2517417‑2517688                                                                                                                                         |                                                                                                                                        ctgcATTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTg                                                                                                                                      >  1:488407/1‑140 (MQ=255)    cATTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCCCACAACACGACAAACGCCTGGCt                                                                                                                                   >  1:1091481/1‑140 (MQ=255)    cATTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCt                                                                                                                                   >  1:149728/1‑140 (MQ=255)     aTTTGATGCGGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGGCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTa                                                                                                                                  <  2:107798/140‑1 (MQ=255)     aTTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTa                                                                                                                                  <  2:613054/140‑1 (MQ=255)      tttGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTAc                                                                                                                                 <  2:283702/140‑1 (MQ=255)       ttGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTAcc                                                                                                                                >  2:352713/1‑140 (MQ=255)            gCCGATCGGTGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCCCACAACACGACAAACGCCTGGCTACCGAGCa                                                                                                                           >  2:848168/1‑140 (MQ=255)            gCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCGGGCACACAACACGACAAACGCCTGGCTACCGAGCa                                                                                                                           >  1:987965/1‑140 (MQ=255)              cGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATg                                                                                                                         >  2:956654/1‑140 (MQ=255)                 tCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCGGGCACACAACACGACAAACGCCTGGCTACCGAGCATGAcc                                                                                                                      <  2:987965/140‑1 (MQ=255)                    gtgTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCa                                                                                                                   <  1:848168/138‑1 (MQ=255)                    gCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCa                                                                                                                   <  1:863674/140‑1 (MQ=255)                    gCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCa                                                                                                                   <  2:279969/140‑1 (MQ=255)                     cGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAg                                                                                                                  >  2:564025/1‑140 (MQ=255)                      gTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGt                                                                                                                 <  1:981114/140‑1 (MQ=255)                      gTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGt                                                                                                                 <  1:617066/140‑1 (MQ=255)                              accaaGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGt                                                                                                                 <  2:883070/132‑1 (MQ=255)                              accaaGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGt                                                                                                                 >  1:883070/1‑132 (MQ=255)                               ccaaGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTaaaaa                                                                                                        <  2:750303/140‑1 (MQ=255)                                  aGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAaga                                                                                                     >  2:731110/1‑140 (MQ=255)                                  aGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAaga                                                                                                     >  1:1061988/1‑140 (MQ=255)                                   gCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAagag                                                                                                    >  1:432613/1‑140 (MQ=255)                                   gCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAagag                                                                                                    >  2:564319/1‑140 (MQ=255)                                       gAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGAt                                                                                                <  1:187966/140‑1 (MQ=255)                                        aaCAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATg                                                                                               >  1:891598/1‑140 (MQ=255)                                             aCGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGGGCAGGGACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAgg                                                                                          >  2:6713/1‑139 (MQ=255)                                             aCGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAGGt                                                                                          >  1:795590/1‑140 (MQ=255)                                             aCGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGt                                                                                          >  1:150224/1‑140 (MQ=255)                                                  gCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCcac                                                                                     >  1:1106110/1‑140 (MQ=255)                                                      gACATATCGGGTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATGCCGCAGGCAATGAACACAACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCGCAGAt                                                                                 <  2:263487/140‑1 (MQ=255)                                                      gACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGAt                                                                                 <  2:150224/140‑1 (MQ=255)                                                      gACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGAt                                                                                 <  1:956654/140‑1 (MQ=255)                                                       aCATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGAtt                                                                                >  1:1152912/1‑140 (MQ=255)                                                           atCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTg                                                                            >  2:359053/1‑140 (MQ=255)                                                           atCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTg                                                                            >  2:997693/1‑140 (MQ=255)                                                                 tGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAAc                                                                      >  1:621965/1‑140 (MQ=255)                                                                  ggTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCTGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACa                                                                     >  1:990209/1‑140 (MQ=255)                                                                  ggTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACa                                                                     >  2:503621/1‑140 (MQ=255)                                                                    tCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATc                                                                   <  1:564025/140‑1 (MQ=255)                                                                    tCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATc                                                                   >  2:667103/1‑140 (MQ=255)                                                                             aaCGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATaaa                                                          >  2:1155480/1‑140 (MQ=255)                                                                             aaCGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATaaa                                                          >  1:409106/1‑140 (MQ=255)                                                                             aaCGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATaaa                                                          >  2:607968/1‑140 (MQ=255)                                                                                  cAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACggg                                                     >  2:333782/1‑140 (MQ=255)                                                                                  cAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACggg                                                     >  1:1088857/1‑140 (MQ=255)                                                                                  cAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACggg                                                     >  1:315091/1‑140 (MQ=255)                                                                                      gcgTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACg                                                 >  1:1117692/1‑140 (MQ=255)                                                                                         tCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTATCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGaca                                              >  1:246108/1‑140 (MQ=255)                                                                                         tCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGaca                                              <  1:930409/140‑1 (MQ=255)                                                                                         tCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGaca                                              >  1:294933/1‑140 (MQ=255)                                                                                           cccAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACAcc                                            >  2:54960/1‑140 (MQ=255)                                                                                              aGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACcgcg                                         <  2:209547/140‑1 (MQ=255)                                                                                              aGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACcgcg                                         <  1:359053/140‑1 (MQ=255)                                                                                               gCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGaca                                              <  1:34519/134‑1 (MQ=255)                                                                                               gCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGaca                                              >  2:34519/1‑134 (MQ=255)                                                                                               gCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCgcgc                                        <  1:820079/140‑1 (MQ=255)                                                                                               gCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCgcgc                                        <  2:1152912/140‑1 (MQ=255)                                                                                                    cAGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCgccagcc                                   <  2:1117692/140‑1 (MQ=255)                                                                                                     aGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACcgcg                                         >  1:781820/1‑133 (MQ=255)                                                                                                     aGCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACcgcg                                         <  2:781820/133‑1 (MQ=255)                                                                                                      gCGTAATATTGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCaa                                 <  2:64029/140‑1 (MQ=255)                                                                                                             atTGCGCAGGCACACAACACGACAAACGCCTGGCTATCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATaaa                          <  2:246108/140‑1 (MQ=255)                                                                                                              ttGCGCAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAAc                         <  2:315091/140‑1 (MQ=255)                                                                                                                 cgcAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAg                      >  1:448988/1‑140 (MQ=255)                                                                                                                 cgcAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAg                      >  2:414969/1‑140 (MQ=255)                                                                                                                 cgcAGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAg                      >  2:182451/1‑140 (MQ=255)                                                                                                                    aGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGAtt                   >  1:681593/1‑140 (MQ=255)                                                                                                                    aGGCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGAATGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGAtt                   <  1:521594/140‑1 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGa                 <  2:149728/140‑1 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGa                 >  2:1114447/1‑140 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGa                 >  2:515230/1‑140 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGa                 >  1:345862/1‑140 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTGCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGa                 <  2:218295/140‑1 (MQ=255)                                                                                                                      gCACACAACACGACAAACGCCTGGCTACCGAGCATGACCGCAGTC‑‑TAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACa               <  1:365341/140‑1 (MQ=255)                                                                                                                                cGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACg       >  2:757563/1‑140 (MQ=255)                                                                                                                                cGACAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACg       >  2:448643/1‑140 (MQ=255)                                                                                                                                  aCAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAATGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGca     <  1:732373/140‑1 (MQ=255)                                                                                                                                  aCAAACGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGca     <  1:667103/140‑1 (MQ=255)                                                                                                                                      aCGCCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAg  <  1:326646/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGCATTTGATGCCGATCGGCGTCACCAAACCAAGCCAGAACAGACGTTGCCAGACATATCGGCTGGTCAATCCATAACGCCAACGCGTCCCCAGCTGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAG  >  NC_000913/2517417‑2517688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑