Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,966	A→G	V65A (GTC→GCC)	ypeB ←	PF12843 family protein YpeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,966	0	A	G	100.0%	176.4 / NA	49	V65A (GTC→GCC)	ypeB	PF12843 family protein YpeB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

TTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCT  >  NC_000913/2527840‑2528100                                                                                                                               |                                                                                                                                       ttCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAg                                                                                                                           <  1:230371/140‑1 (MQ=255)    cATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGccc                                                                                                                        <  1:927978/140‑1 (MQ=255)     aTCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGcccc                                                                                                                       >  1:24977/1‑140 (MQ=255)     aTCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTAAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGcccc                                                                                                                       >  2:16898/1‑140 (MQ=255)         gATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCgg                                                                                                                   <  1:817858/140‑1 (MQ=255)                ttGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAAt                                                                                                            <  2:386201/140‑1 (MQ=255)                     ggTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATcagca                                                                                                       >  1:847941/1‑140 (MQ=255)                        cTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCg                                                                                                    <  1:16898/140‑1 (MQ=255)                          gtgCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCagcagc                                                                                                     >  2:937405/1‑137 (MQ=255)                          gtgCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCagcagc                                                                                                     <  1:937405/137‑1 (MQ=255)                          gtgCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTg                                                                                                  >  1:394754/1‑140 (MQ=255)                                tGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGc                                                                                            >  1:50587/1‑140 (MQ=255)                                 gCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCa                                                                                           <  1:53377/140‑1 (MQ=255)                                     gcgcGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCagcagc                                                                                                     >  2:429608/1‑126 (MQ=255)                                     gcgcGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCagcagc                                                                                                     <  1:429608/126‑1 (MQ=255)                                     gcgcGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAg                                                                                       <  2:24977/140‑1 (MQ=255)                                     gcgcGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAg                                                                                       >  2:838715/1‑140 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGa                                                                                                 >  2:1138644/1‑125 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGa                                                                                                 <  1:1138644/125‑1 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCAc                                                                                  >  1:119534/1‑140 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCAc                                                                                  >  1:981747/1‑140 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCAc                                                                                  >  1:943465/1‑140 (MQ=255)                                          tCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCAc                                                                                  >  1:633087/1‑140 (MQ=255)                                              tACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAg                                                                              <  1:808287/140‑1 (MQ=255)                                                        aGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGcc                                                                    <  2:394754/140‑1 (MQ=255)                                                        aGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGcc                                                                    >  1:671609/1‑140 (MQ=255)                                                         gACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg                                                                   >  2:289558/1‑140 (MQ=255)                                                             gAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGaa                                                               <  1:751758/140‑1 (MQ=255)                                                                     aCAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCtt                                                       >  1:756460/1‑140 (MQ=255)                                                                        ggCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGc                                                    >  2:660385/1‑140 (MQ=255)                                                                           gcagcaGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCggg                                                 <  2:50587/140‑1 (MQ=255)                                                                            cagcagGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGc                                                >  2:444253/1‑140 (MQ=255)                                                                             agcagGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCa                                               >  2:937944/1‑140 (MQ=255)                                                                             agcagGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCa                                               >  2:860236/1‑140 (MQ=255)                                                                                  gAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACca                                          <  2:584094/140‑1 (MQ=255)                                                                                   aaaGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACcac                                         <  2:756460/140‑1 (MQ=255)                                                                                    aaGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACcaca                                        <  1:444253/140‑1 (MQ=255)                                                                                    aaGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTCTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACcaca                                        <  2:1096078/140‑1 (MQ=255)                                                                                         gcgcCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa                                   <  2:671609/140‑1 (MQ=255)                                                                                          cgcCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCaaa                                             >  1:38488/1‑129 (MQ=255)                                                                                          cgcCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCaaa                                             <  2:38488/129‑1 (MQ=255)                                                                                              gATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATActc                              <  1:911588/140‑1 (MQ=255)                                                                                               aTAAAGCTTAAAGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATActcc                             >  2:135657/1‑140 (MQ=255)                                                                                                        aaaGCGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAgg                    <  1:660385/140‑1 (MQ=255)                                                                                                            cGGACGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGa                <  2:943465/140‑1 (MQ=255)                                                                                                               aCGCTTCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTa             >  1:311704/1‑140 (MQ=255)                                                                                                                    tCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGa        <  2:633087/140‑1 (MQ=255)                                                                                                                    tCAGGGGCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGa        <  2:311704/140‑1 (MQ=255)                                                                                                                          gCTGGGCCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCt  <  2:1146645/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       TTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCT  >  NC_000913/2527840‑2528100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑