Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,452	T→C	F62L (TTC→CTC)	nlpE →	lipoprotein NlpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,452	0	T	C	100.0%	350.9 / NA	96	F62L (TTC→CTC)	nlpE	lipoprotein NlpE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/48);  total (48/48)

TTACTCTGATGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGTTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGAAA  >  NC_000913/215315‑215587                                                                                                                                          |                                                                                                                                        ttACTCTGATGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTc                                                                                                                                       >  1:356872/1‑140 (MQ=255)         aTGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCTATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaa                                                                                                                               >  1:690471/1‑140 (MQ=255)         aTGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGATACCTCTCTGCTCCTCGaaaa                                                                                                                               >  1:850612/1‑140 (MQ=255)         aTGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaa                                                                                                                               >  1:706823/1‑140 (MQ=255)         aTGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaa                                                                                                                               >  2:843301/1‑140 (MQ=255)         aTGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaa                                                                                                                               >  1:40363/1‑140 (MQ=255)          tGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaaa                                                                                                                              >  2:199388/1‑140 (MQ=255)          tGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaaa                                                                                                                              >  2:503630/1‑140 (MQ=255)          tGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaaa                                                                                                                              >  1:422448/1‑140 (MQ=255)          tGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGaaaaa                                                                                                                              >  1:243557/1‑140 (MQ=255)            ggATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCTATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGa                                                                                                                            <  2:690471/140‑1 (MQ=255)            ggATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGa                                                                                                                            <  2:40363/140‑1 (MQ=255)            ggATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGa                                                                                                                            <  2:585555/140‑1 (MQ=255)            ggATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGa                                                                                                                            <  1:317709/140‑1 (MQ=255)                   ataatCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACa                                                                                                                     <  2:706823/140‑1 (MQ=255)                   ataatCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACa                                                                                                                     <  2:734105/140‑1 (MQ=255)                      atCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATgg                                                                                                                  >  2:483461/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  2:1162288/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  1:482097/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  1:57586/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  2:145120/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  2:629043/1‑140 (MQ=255)                       tCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATggg                                                                                                                 >  2:884992/1‑140 (MQ=255)                        cGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGt                                                                                                                >  1:551807/1‑140 (MQ=255)                        cGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGt                                                                                                                >  2:960822/1‑140 (MQ=255)                        cGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCACAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGt                                                                                                                >  2:192333/1‑140 (MQ=255)                         gggCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGtg                                                                                                               >  2:1073772/1‑140 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATg                                                                                                                   <  1:1028600/134‑1 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATg                                                                                                                   >  2:1028600/1‑134 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGtgat                                                                                                             >  1:1108869/1‑140 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGtgat                                                                                                             >  2:503111/1‑140 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGtgat                                                                                                             >  1:259201/1‑140 (MQ=255)                           gCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGtgat                                                                                                             >  1:52997/1‑140 (MQ=255)                              gAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGatgaa                                                                                                          <  1:884992/140‑1 (MQ=255)                               aaGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGatgaat                                                                                                         <  1:960822/140‑1 (MQ=255)                               aaGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGatgaat                                                                                                         <  1:1032929/140‑1 (MQ=255)                               aaGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGatgaat                                                                                                         <  1:107182/140‑1 (MQ=255)                               aaGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGatgaat                                                                                                         <  1:503630/140‑1 (MQ=255)                                  tCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAg                                                                                                      >  1:191883/1‑140 (MQ=255)                                    gATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCg                                                                                                    >  1:58111/1‑140 (MQ=255)                                    gATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCg                                                                                                    <  2:52997/140‑1 (MQ=255)                                          cTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTAtct                                                                                              <  2:551102/140‑1 (MQ=255)                                          cTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTAtct                                                                                              <  1:199388/140‑1 (MQ=255)                                          cTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTAtct                                                                                              <  1:767952/140‑1 (MQ=255)                                          cTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTAtct                                                                                              <  2:811940/140‑1 (MQ=255)                                           tttCTCCGACGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTAtctc                                                                                             >  2:286749/1‑140 (MQ=255)                                             tctcCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCgg                                                                                           <  2:760591/140‑1 (MQ=255)                                               tcCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTg                                                                                         >  1:393543/1‑140 (MQ=255)                                               tcCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTg                                                                                         <  1:932611/140‑1 (MQ=255)                                                        ggCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTgaag                                                                                <  1:843301/140‑1 (MQ=255)                                                             ccGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCt                                                                           >  2:211523/1‑140 (MQ=255)                                                                     aaaCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCtt                                                                   >  2:755290/1‑140 (MQ=255)                                                                     aaaCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCtt                                                                   >  2:1103272/1‑140 (MQ=255)                                                                       aCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTg                                                                                   <  2:729338/122‑1 (MQ=255)                                                                       aCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTg                                                                                   <  2:262155/122‑1 (MQ=255)                                                                       aCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTg                                                                                   >  1:729338/1‑122 (MQ=255)                                                                       aCCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTg                                                                                   >  1:262155/1‑122 (MQ=255)                                                                         cGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCt                                                               <  1:1162288/140‑1 (MQ=255)                                                                           aTGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTc                                                             >  2:1018982/1‑140 (MQ=255)                                                                           aTGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTc                                                             <  1:423234/140‑1 (MQ=255)                                                                            tGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCg                                                                 <  2:963785/135‑1 (MQ=255)                                                                            tGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCg                                                                 >  1:963785/1‑135 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  1:286749/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  2:57586/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  1:503111/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  1:145120/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  2:494657/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  2:191883/140‑1 (MQ=255)                                                                              ccGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTa                                                          <  2:259201/140‑1 (MQ=255)                                                                               cGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTAc                                                         >  1:885034/1‑140 (MQ=255)                                                                                        ggCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATgg                                                <  1:1103272/140‑1 (MQ=255)                                                                                             ggcgTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGcgcg                                           <  2:482097/140‑1 (MQ=255)                                                                                              gcgTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGa                                          >  2:415655/1‑140 (MQ=255)                                                                                                      ccGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGa                                  <  1:629043/140‑1 (MQ=255)                                                                                                      ccGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGa                                  <  2:551807/140‑1 (MQ=255)                                                                                                      ccGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGa                                  <  1:184799/140‑1 (MQ=255)                                                                                                          gtgCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAg                              <  1:1087482/140‑1 (MQ=255)                                                                                                           tgCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGc                             >  2:415272/1‑140 (MQ=255)                                                                                                           tgCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGc                             >  2:134358/1‑140 (MQ=255)                                                                                                            gCCGATTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCt                            <  2:1108869/140‑1 (MQ=255)                                                                                                                aTTGCGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTa                        >  1:1041594/1‑140 (MQ=255)                                                                                                                    cGAAGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTaa                    >  2:682712/1‑140 (MQ=255)                                                                                                                       aGGAATCGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGTGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCg                 >  1:130887/1‑140 (MQ=255)                                                                                                                             cGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGTGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCa           <  2:130887/140‑1 (MQ=255)                                                                                                                             cGAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCa           <  1:1073772/140‑1 (MQ=255)                                                                                                                              gAAACCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCaa          <  2:58111/140‑1 (MQ=255)                                                                                                                               aaaCCTCTCTGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCaaa         <  2:376720/140‑1 (MQ=255)                                                                                                                                   ctctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAAcc                  >  1:875217/1‑127 (MQ=255)                                                                                                                                   ctctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAAcc                  <  2:875217/127‑1 (MQ=255)                                                                                                                                   ctctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTg     >  2:673096/1‑140 (MQ=255)                                                                                                                                   ctctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTg     >  1:203632/1‑140 (MQ=255)                                                                                                                                    tctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGa    <  2:850612/140‑1 (MQ=255)                                                                                                                                     ctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGaa   <  1:415272/140‑1 (MQ=255)                                                                                                                                     ctctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGaa   <  1:134358/140‑1 (MQ=255)                                                                                                                                      tctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGaaa  >  1:386187/1‑140 (MQ=255)                                                                                                                                      tctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGaaa  >  1:128032/1‑140 (MQ=255)                                                                                                                                      tctGCTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGaaa  <  2:393543/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTACTCTGATGGGATGTAATAATCGGGCCGAAGTCGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCCGATTGCGAAGGAATCGAAACCTCTCTGTTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAAGAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGAAA  >  NC_000913/215315‑215587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑