Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,616,960	T→C	R289R (CGT→CGC)	bepA →	beta‑barrel assembly‑enhancing protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,616,960	0	T	C	100.0%	221.4 / NA	61	R289R (CGT→CGC)	bepA	beta‑barrel assembly‑enhancing protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/27);  total (34/27)

ACCCGTTGCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGTAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCG  >  NC_000913/2616830‑2617095                                                                                                                                   |                                                                                                                                        aCCCGTTGCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTc                                                                                                                                >  1:180728/1‑140 (MQ=255)       tGCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAAc                                                                                                                                      <  1:1049824/128‑1 (MQ=255)       tGCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAAc                                                                                                                                      >  2:1049824/1‑128 (MQ=255)        gCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTg                                                                                                                         >  1:1088578/1‑140 (MQ=255)          cGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGAt                                                                                                                       <  1:442161/140‑1 (MQ=255)             aaaGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTg                                                                                                                    <  1:620383/140‑1 (MQ=255)             aaaGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTg                                                                                                                    >  2:916614/1‑140 (MQ=255)             aaaGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTg                                                                                                                    >  2:236911/1‑140 (MQ=255)                      tGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGaa                                                                                                           <  1:916614/140‑1 (MQ=255)                          aGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATggg                                                                                                       <  1:1019797/140‑1 (MQ=255)                           gATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTgg                                                                                                                <  1:263418/130‑1 (MQ=255)                           gATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTgg                                                                                                                >  2:263418/1‑130 (MQ=255)                               cccGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGaaa                                                                                                  <  1:236911/140‑1 (MQ=255)                                  gCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGa                                                                                               >  2:300596/1‑140 (MQ=255)                                   cAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGaa                                                                                              <  2:1088745/140‑1 (MQ=255)                                      ccGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACg                                                                                           <  1:626012/140‑1 (MQ=255)                                             aaTCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCa                                                                                    >  2:319621/1‑140 (MQ=255)                                              aTCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAg                                                                                   >  1:1154566/1‑140 (MQ=255)                                                cAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCa                                                                                 <  2:590029/140‑1 (MQ=255)                                                     gcgcCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGag                                                                            >  2:299992/1‑140 (MQ=255)                                                        cccGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         <  2:325723/140‑1 (MQ=255)                                                        cccGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         >  2:425004/1‑140 (MQ=255)                                                        cccGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         >  2:954209/1‑140 (MQ=255)                                                        cccGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         <  1:425004/140‑1 (MQ=255)                                                               gtggtgCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAata                                                                  <  2:180728/140‑1 (MQ=255)                                                                    gCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTc                                                             <  1:300596/140‑1 (MQ=255)                                                                     cAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCg                                                            <  1:299992/140‑1 (MQ=255)                                                                       gtcgtcGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         >  2:784274/1‑125 (MQ=255)                                                                       gtcgtcGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGagcag                                                                         <  1:784274/125‑1 (MQ=255)                                                                       gtcgtcGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTg                                                          >  1:1027944/1‑140 (MQ=255)                                                                       gtcgtcGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTg                                                          <  2:1068195/140‑1 (MQ=255)                                                                        tcgtcgGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc                                                         >  1:4040/1‑140 (MQ=255)                                                                         cgtcgGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCt                                                        >  1:2024/1‑140 (MQ=255)                                                                          gtcgGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCtt                                                       >  2:61648/1‑140 (MQ=255)                                                                          gtcgGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCtt                                                       >  1:743527/1‑140 (MQ=255)                                                                              gAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACa                                                   <  1:954209/140‑1 (MQ=255)                                                                                   tttCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGa                                              <  1:378292/140‑1 (MQ=255)                                                                                      cTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATgg                                           >  1:170012/1‑140 (MQ=255)                                                                                         tCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAg                                        <  2:806874/140‑1 (MQ=255)                                                                                         tCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAg                                        >  1:806874/1‑140 (MQ=255)                                                                                             gCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaa                                    <  2:743527/140‑1 (MQ=255)                                                                                             gCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaa                                    >  1:411403/1‑140 (MQ=255)                                                                                                  aGCGCGCACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaataaat                               <  2:37177/140‑1 (MQ=255)                                                                                                       gcACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaat                                   <  1:1025703/131‑1 (MQ=255)                                                                                                       gcACACTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaat                                   >  2:1025703/1‑131 (MQ=255)                                                                                                          cacTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAg                       <  2:1154566/140‑1 (MQ=255)                                                                                                          cacTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAg                       <  2:411403/140‑1 (MQ=255)                                                                                                           acTGGGGATGTATAATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAgg                      <  2:4040/140‑1 (MQ=255)                                                                                                                     tataATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAAc            >  2:166323/1‑140 (MQ=255)                                                                                                                     tataATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAAc            >  1:1079524/1‑140 (MQ=255)                                                                                                                     tataATTCCGGACGCAACCAGCTCACCAGTGATTNGCTGGATGAATGGGCAAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAAc            >  1:290430/1‑140 (MQ=255)                                                                                                                      ataATTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACg           >  1:1064802/1‑140 (MQ=255)                                                                                                                         aTTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTg        >  1:935675/1‑140 (MQ=255)                                                                                                                         aTTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTg        >  1:453493/1‑140 (MQ=255)                                                                                                                         aTTCCGGACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATCGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTg        >  1:468438/1‑140 (MQ=255)                                                                                                                              ggACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGc       <  2:708727/136‑1 (MQ=255)                                                                                                                              ggACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGc       >  1:708727/1‑136 (MQ=255)                                                                                                                              ggACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCAAATAAATACGACGAGGCGCGTAAAACGCTGCatac   >  2:570460/1‑137 (MQ=255)                                                                                                                               gACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCg  >  2:659926/1‑140 (MQ=255)                                                                                                                               gACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCg  >  1:178770/1‑140 (MQ=255)                                                                                                                               gACGCAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCg  >  2:785522/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        ACCCGTTGCCGGAAAGTCGTCTGGCAGATGCCCGCAACCGTGCTAATCAGATGCGCCCGATGGTGGTGCAGTCGTCGGAAGATTTCTATCTGGCGAAAGCGCGCACACTGGGGATGTATAATTCCGGACGTAACCAGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCG  >  NC_000913/2616830‑2617095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑