Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,634,737	T→C	V169A (GTG→GCG)	xseA →	exodeoxyribonuclease VII subunit XseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,634,737	0	T	C	100.0%	189.1 / NA	53	V169A (GTG→GCG)	xseA	exodeoxyribonuclease VII subunit XseA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/20);  total (33/20)

AAGGTTTGTTCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGTGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATG  >  NC_000913/2634602‑2634872                                                                                                                                        |                                                                                                                                        aaGGTTTGTTCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGatc                                                                                                                                     >  2:770838/1‑140 (MQ=255)    gTTTGTTCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGatcatc                                                                                                                                  <  2:555590/140‑1 (MQ=255)       tGTTCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTAc                                                                                                                               >  1:375150/1‑140 (MQ=255)         ttCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTAccc                                                                                                                             <  1:1060869/140‑1 (MQ=255)         ttCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTAccc                                                                                                                             >  2:688382/1‑140 (MQ=255)              tCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTgccg                                                                                                                        >  2:915881/1‑140 (MQ=255)                   aaTACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGtt                                                                                                                   <  2:1122536/140‑1 (MQ=255)                       cAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAgg                                                                                                               >  1:1063284/1‑140 (MQ=255)                        aaaaaaCCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCCGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAggg                                                                                                              <  2:1150598/140‑1 (MQ=255)                                 cTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGAcgc                                                                                                     >  2:376011/1‑140 (MQ=255)                                    ccctcccCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGcc                                                                                                  <  1:915881/140‑1 (MQ=255)                                              cgcATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAAtt                                                                                        >  2:1016605/1‑140 (MQ=255)                                                   tGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTcg                                                                                   <  1:1016605/140‑1 (MQ=255)                                                   tGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTcg                                                                                   >  1:1018635/1‑140 (MQ=255)                                                    gCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTCCCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTcgc                                                                                  <  2:144540/140‑1 (MQ=255)                                                     cGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCaaa                                                                                          <  1:1100991/131‑1 (MQ=255)                                                     cGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCaaa                                                                                          >  2:1100991/1‑131 (MQ=255)                                                             tgATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGaa                                                                         >  1:686422/1‑140 (MQ=255)                                                                 cACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTgg                                                                     <  1:519099/140‑1 (MQ=255)                                                                  aCCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGc                                                                    >  2:670138/1‑140 (MQ=255)                                                                    cTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGa                                                                          >  2:1065450/1‑132 (MQ=255)                                                                    cTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGa                                                                          <  1:1065450/132‑1 (MQ=255)                                                                    cTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGa                                                                  >  1:145817/1‑140 (MQ=255)                                                                    cTCAAAAACCGGTGATGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTgg                                                                     >  2:505419/1‑137 (MQ=255)                                                                    cTCAAAAACCGGTGATGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTgg                                                                     <  1:505419/137‑1 (MQ=255)                                                                      cAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAAt                                                                <  1:670138/140‑1 (MQ=255)                                                                             cGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCa                                                         <  2:1063284/140‑1 (MQ=255)                                                                                      gcTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCg                                                <  2:1018635/140‑1 (MQ=255)                                                                                          cATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGa                                                     >  2:927790/1‑131 (MQ=255)                                                                                          cATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGa                                                     <  1:927790/131‑1 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  2:1042572/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  2:1003536/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  1:991856/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  2:903113/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  1:195333/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  1:785351/1‑140 (MQ=255)                                                                                                    tGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGAtcgtc                                  >  2:534659/1‑140 (MQ=255)                                                                                                         gtgtTAAAACGTCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGgcg                             >  2:104359/1‑140 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGcgcg                           <  2:108012/131‑1 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGcgcg                           >  1:108012/1‑131 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGtt                  <  1:770838/140‑1 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGtt                  >  1:16162/1‑140 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGtt                  <  2:785351/140‑1 (MQ=255)                                                                                                                    tCGCGATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGtt                  >  1:137665/1‑140 (MQ=255)                                                                                                                     cgcgATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTc                 >  1:40787/1‑140 (MQ=255)                                                                                                                     cgcgATCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTc                 >  1:351393/1‑140 (MQ=255)                                                                                                                         aTCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTg             >  1:666446/1‑140 (MQ=255)                                                                                                                          tCCTTCTCTGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTgg            <  2:686422/140‑1 (MQ=255)                                                                                                                               ctctGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGaa          >  2:172801/1‑137 (MQ=255)                                                                                                                               ctctGCCGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGaa          <  1:172801/137‑1 (MQ=255)                                                                                                                                    ccGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATg  >  2:598142/1‑140 (MQ=255)                                                                                                                                    ccGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATg  >  1:81657/1‑140 (MQ=255)                                                                                                                                    ccGGCGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATg  >  2:684779/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        AAGGTTTGTTCGATCAGCAATACAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATTTTGCATGTGTTAAAACGTCGCGATCCTTCTCTGCCGGTGATCATCTACCCTGCCGCCGTTCAGGGCGATGACGCGCCGGGGCAAATTGTTCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATG  >  NC_000913/2634602‑2634872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑