Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,666,070	A→G	A212A (GCA→GCG)	csiE →	stationary phase inducible protein CsiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,666,070	0	A	G	100.0%	177.9 / NA	49	A212A (GCA→GCG)	csiE	stationary phase inducible protein CsiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

ACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAG  >  NC_000913/2665936‑2666205                                                                                                                                       |                                                                                                                                        aCGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCgctact                                                                                                                                    >  2:169856/1‑140 (MQ=255)  cGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACtt                                                                                                                                   >  1:1143582/1‑140 (MQ=255)  cGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACtt                                                                                                                                   >  2:550698/1‑140 (MQ=255)               tCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGc                                                                                                                      >  1:225404/1‑140 (MQ=255)                ccGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCg                                                                                                                     <  2:191309/140‑1 (MQ=255)                 cGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCgg                                                                                                                    <  1:501495/140‑1 (MQ=255)                 cGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCgg                                                                                                                    <  2:231154/140‑1 (MQ=255)                    tCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGtt                                                                                                                 >  1:460701/1‑140 (MQ=255)                        aCGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCgg                                                                                                                    <  2:158105/133‑1 (MQ=255)                        aCGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCgg                                                                                                                    >  1:158105/1‑133 (MQ=255)                          gTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGAt                                                                                                           <  1:315798/140‑1 (MQ=255)                          gTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGAt                                                                                                           <  2:1143582/140‑1 (MQ=255)                                    ggAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCggg                                                                                                 <  2:603466/140‑1 (MQ=255)                                        tCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATgc                                                                                             >  1:909879/1‑140 (MQ=255)                                            gCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCAt                                                                                         <  2:229524/140‑1 (MQ=255)                                               ttGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAg                                                                                      <  2:460701/140‑1 (MQ=255)                                                         ggTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAACCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagac                                                                            <  2:491318/140‑1 (MQ=255)                                                              cgcAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTg                                                                       >  2:280313/1‑140 (MQ=255)                                                              cgcAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTg                                                                       >  2:1048794/1‑140 (MQ=255)                                                              cgcAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTg                                                                       >  2:835826/1‑140 (MQ=255)                                                               gcAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGc                                                                      >  2:196701/1‑140 (MQ=255)                                                                  ggAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAAATGCGTc                                                                   <  2:237737/140‑1 (MQ=255)                                                                   gAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCa                                                                  <  2:81751/140‑1 (MQ=255)                                                                        tGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTa                                                             >  1:834777/1‑140 (MQ=255)                                                                         ggACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTAt                                                            <  1:774965/140‑1 (MQ=255)                                                                             gCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCaaa                                                        >  2:330364/1‑140 (MQ=255)                                                                               cACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACg                                                      >  2:355743/1‑140 (MQ=255)                                                                                         gTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAAc                                            >  1:682228/1‑140 (MQ=255)                                                                                         gTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAAc                                            >  2:382220/1‑140 (MQ=255)                                                                                                     aGCCTGTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAg                                <  1:330364/140‑1 (MQ=255)                                                                                                          gTACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTggg                           <  1:835826/140‑1 (MQ=255)                                                                                                           tACCACCTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGa                          >  1:644314/1‑140 (MQ=255)                                                                                                            accaccTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTc                                     <  2:1001275/128‑1 (MQ=255)                                                                                                            accaccTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTc                                     >  1:1001275/1‑128 (MQ=255)                                                                                                                ccTGATGAGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGt                     >  1:295126/1‑140 (MQ=255)                                                                                                                   gatgaGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCg                  <  2:295126/140‑1 (MQ=255)                                                                                                                     tgaGCCACTGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGtt                >  2:874260/1‑140 (MQ=255)                                                                                                                             tGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgat        >  1:712496/1‑140 (MQ=255)                                                                                                                             tGTTTATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgat        <  2:909879/140‑1 (MQ=255)                                                                                                                                ttATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     <  2:644314/140‑1 (MQ=255)                                                                                                                                ttATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     <  1:874260/140‑1 (MQ=255)                                                                                                                                ttATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     <  2:840061/140‑1 (MQ=255)                                                                                                                                ttATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     <  2:225404/140‑1 (MQ=255)                                                                                                                                 tATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAAc    >  2:985722/1‑140 (MQ=255)                                                                                                                                 tATGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAAc    <  1:1048794/140‑1 (MQ=255)                                                                                                                                   tGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     >  2:314194/1‑137 (MQ=255)                                                                                                                                   tGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa     <  1:314194/137‑1 (MQ=255)                                                                                                                                   tGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAg  <  2:834777/140‑1 (MQ=255)                                                                                                                                   tGGCGCTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAg  >  2:380542/1‑140 (MQ=255)                                                                                                                                   tGGCGCTACTTTTTTCCATGTTGCGGGTTCACGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAg  <  2:712496/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAG  >  NC_000913/2665936‑2666205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑