Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,816	T→C	H140R (CAC→CGC)	ygbI ←	putative DeoR‑type DNA‑binding transcriptional regulator YgbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,816	0	T	C	100.0%	244.8 / NA	67	H140R (CAC→CGC)	ygbI	putative DeoR‑type DNA‑binding transcriptional regulator YgbI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/34);  total (33/34)

CGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCAGATAGATACA  >  NC_000913/2860680‑2860952                                                                                                                                         |                                                                                                                                         cGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGaa                                                                                                                                       >  1:1009980/1‑140 (MQ=255) cGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGaa                                                                                                                                       >  2:1144276/1‑140 (MQ=255) cGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGGCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGGCACACTGCACCGCCAGTGCGaa                                                                                                                                       >  2:410411/1‑140 (MQ=255)   cTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGaata                                                                                                                                     >  2:919624/1‑140 (MQ=255)     ggCGTAGAAATCCCCCGCCCCCCCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGaataat                                                                                                                                   <  2:99583/140‑1 (MQ=255)     ggCGTAGAAACCCCCCGCACCCCCCATGACGATGCAAAAATAAAAGCTTGATAAATCATCAGGCTGCGCAGCATGGTCGCGGCGGCTCCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGaataat                                                                                                                                   <  2:1120810/140‑1 (MQ=255)       cagaGAAATCCCCCGCCCCCCCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTCCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATtg                                                                                                                                 <  2:1095977/137‑1 (MQ=255)        gTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATAAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATtgt                                                                                                                                <  1:638042/140‑1 (MQ=255)           gAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATtgt                                                                                                                                <  2:355922/137‑1 (MQ=255)           gAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATtgt                                                                                                                                >  1:355922/1‑137 (MQ=255)                 ccccGCCCACTCCAGGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCCCAGCATGGCCGCAGCGGCTCCCCCAACACAGGAACGGTTTCCCCGACACACTCCACCGCCAGTGCGAATAATTGTGCAATTACt                                                                                                                       <  2:733831/140‑1 (MQ=255)                  ccccCACCCCCCATGACGATGCAAAAATAAAACCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACtg                                                                                                                      <  2:890486/140‑1 (MQ=255)                     gCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTActgctg                                                                                                                   >  1:1095977/1‑140 (MQ=255)                            ccATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCa                                                                                                            >  1:780099/1‑140 (MQ=255)                                  cGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAagtagt                                                                                                      <  1:702203/140‑1 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAag                                                                                                          <  2:487725/133‑1 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAag                                                                                                          >  1:487725/1‑133 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAag                                                                                                          <  2:594672/133‑1 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAag                                                                                                          >  1:594672/1‑133 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCg                                                                                                   >  1:5679/1‑140 (MQ=255)                                     tGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCg                                                                                                   >  2:838490/1‑140 (MQ=255)                                                   ccTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCg                                                                                     <  1:772630/140‑1 (MQ=255)                                                    cTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAAt                                                                                       <  2:245523/137‑1 (MQ=255)                                                    cTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAAt                                                                                       >  1:245523/1‑137 (MQ=255)                                                    cTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGt                                                                                    >  1:865350/1‑140 (MQ=255)                                                    cTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGt                                                                                    >  2:553317/1‑140 (MQ=255)                                                      gATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGttt                                                                                  >  2:494681/1‑140 (MQ=255)                                                      gATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGttt                                                                                  >  1:93242/1‑140 (MQ=255)                                                       atcaatcaTCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTtg                                                                                 >  1:876193/1‑140 (MQ=255)                                                       atcaatcaTCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTtg                                                                                 >  2:227235/1‑140 (MQ=255)                                                           atcatcAGGCTGCGCAGCATGGCCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGAc                                                                             <  1:77538/140‑1 (MQ=255)                                                               tcaGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACcaca                                                                         <  2:5679/140‑1 (MQ=255)                                                                 aGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagt                                                                       >  2:237496/1‑140 (MQ=255)                                                                 aGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTCGTGACCAcagt                                                                       >  2:95188/1‑140 (MQ=255)                                                                    cTGCGCAGCATGGTCGCGGCGGCTCCCCCGACACAGGAACGTTTTCCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcag                                                                    <  2:865350/140‑1 (MQ=255)                                                                     tGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcagt                                                                   <  1:237496/140‑1 (MQ=255)                                                                       cgcAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCGAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGa                                                                 >  2:284254/1‑140 (MQ=255)                                                                       cgcAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGa                                                                 >  1:39538/1‑140 (MQ=255)                                                                       cgcAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGa                                                                 >  2:287381/1‑140 (MQ=255)                                                                             aTGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACt                                                               >  1:683110/1‑136 (MQ=255)                                                                             aTGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACt                                                               <  2:683110/136‑1 (MQ=255)                                                                               ggTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgt                                                         <  1:553317/140‑1 (MQ=255)                                                                                 tCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa                                                       <  1:931930/140‑1 (MQ=255)                                                                                 tCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa                                                       >  2:931930/1‑140 (MQ=255)                                                                                      gCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAg                                                  >  1:311680/1‑140 (MQ=255)                                                                                       cGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGa                                                 <  1:494681/140‑1 (MQ=255)                                                                                              cccGACACAGGAACGTTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCtgtg                                          <  2:1009980/140‑1 (MQ=255)                                                                                               ccGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGc                                         <  2:93242/140‑1 (MQ=255)                                                                                               ccGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGc                                         <  1:1144276/140‑1 (MQ=255)                                                                                               ccGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGc                                         <  2:876193/140‑1 (MQ=255)                                                                                               ccGACAAAGGAACGGTTTTCCCGGCACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGc                                         <  1:410411/140‑1 (MQ=255)                                                                                                    acaGGAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCg                                    <  2:474526/140‑1 (MQ=255)                                                                                                        gAACGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTaa                                >  1:192915/1‑140 (MQ=255)                                                                                                         aaCGGTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAAc                               <  2:1148414/140‑1 (MQ=255)                                                                                                             gTTTTCCCGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTgg                           >  1:74859/1‑140 (MQ=255)                                                                                                                    cGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTcc                    <  1:919624/140‑1 (MQ=255)                                                                                                                    cGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTcc                    <  1:838490/140‑1 (MQ=255)                                                                                                                    cGACACACTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTcc                    <  2:8854/140‑1 (MQ=255)                                                                                                                          ccggCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATc              <  1:534739/137‑1 (MQ=255)                                                                                                                          acTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATc              <  1:227235/140‑1 (MQ=255)                                                                                                                          acTGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATc              >  1:932241/1‑140 (MQ=255)                                                                                                                            tGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCa            >  1:312216/1‑140 (MQ=255)                                                                                                                            tGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCa            >  1:1160687/1‑140 (MQ=255)                                                                                                                            tGCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCa            >  1:202205/1‑140 (MQ=255)                                                                                                                             gCACCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCag           >  1:2013/1‑140 (MQ=255)                                                                                                                               aCCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCagat         <  1:122110/140‑1 (MQ=255)                                                                                                                               aCCGCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCagat         <  2:311680/140‑1 (MQ=255)                                                                                                                                  gCCAGTGCGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCagataga      >  2:770265/1‑140 (MQ=255)                                                                                                                                      gtgcGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCAGATAGATACa  >  1:623799/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCAGATAGATACA  >  NC_000913/2860680‑2860952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑