Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,974	T→C	T103A (ACC→GCC)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,974	0	T	C	100.0%	261.1 / NA	72	T103A (ACC→GCC)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

ATTACACACCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCC  >  NC_000913/2924837‑2925107                                                                                                                                          |                                                                                                                                      aTTACACACCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGctt                                                                                                                                     >  1:962944/1‑140 (MQ=255)     cacacCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTgcag                                                                                                                                 <  1:80949/140‑1 (MQ=255)       cacCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTgcagca                                                                                                                               >  2:20674/1‑140 (MQ=255)        acCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCaa                                                                                                                              >  1:387623/1‑140 (MQ=255)          cGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCTGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATg                                                                                                                            >  1:443353/1‑140 (MQ=255)          cGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATg                                                                                                                            <  1:20674/140‑1 (MQ=255)          cGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATg                                                                                                                            >  2:265241/1‑140 (MQ=255)           gagaTGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGt                                                                                                                           >  1:923548/1‑140 (MQ=255)            agaTGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTc                                                                                                                          >  1:1009549/1‑140 (MQ=255)            agaTGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTc                                                                                                                          >  2:314833/1‑140 (MQ=255)                  ccTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTg                                                                                                                    >  1:348976/1‑140 (MQ=255)                            ttcttcCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCaa                                                                                                          >  2:493921/1‑140 (MQ=255)                             tcttcCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCaaa                                                                                                         >  1:1145626/1‑140 (MQ=255)                             tcttcCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCaaa                                                                                                         >  1:371335/1‑140 (MQ=255)                              cttcCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAAc                                                                                                        <  2:327776/140‑1 (MQ=255)                                 ccAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgc                                                                                                     <  2:1009549/140‑1 (MQ=255)                                 ccAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgc                                                                                                     <  2:160572/140‑1 (MQ=255)                                  cAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgcg                                                                                                    <  1:634388/140‑1 (MQ=255)                                  cAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgcg                                                                                                    <  1:493921/140‑1 (MQ=255)                                  cAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgcg                                                                                                    <  1:202837/140‑1 (MQ=255)                                   aGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTgcgc                                                                                                   <  1:314833/140‑1 (MQ=255)                                    gCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGcgcg                                                                                                  >  2:278707/1‑140 (MQ=255)                                        cgcgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCa                                                                                              >  1:59406/1‑140 (MQ=255)                                        cgcgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCa                                                                                              >  2:1049340/1‑140 (MQ=255)                                        cgcgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCa                                                                                              >  2:849456/1‑140 (MQ=255)                                          cgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCata                                                                                            >  2:1105894/1‑140 (MQ=255)                                                     gtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCaaa                                                                                 >  2:451226/1‑140 (MQ=255)                                                     gtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCaaa                                                                                 >  2:642702/1‑140 (MQ=255)                                                      tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAAc                                                                                >  2:733203/1‑140 (MQ=255)                                                      tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAAc                                                                                >  2:1027054/1‑140 (MQ=255)                                                      tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAAc                                                                                >  1:768623/1‑140 (MQ=255)                                                      tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAAc                                                                                >  2:411973/1‑140 (MQ=255)                                                      tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAAc                                                                                >  2:446812/1‑140 (MQ=255)                                                       gggCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACt                                                                               <  1:278707/140‑1 (MQ=255)                                                       gggCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACt                                                                               <  1:919704/140‑1 (MQ=255)                                                        ggcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTg                                                                              >  2:946701/1‑140 (MQ=255)                                                         gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGa                                                                             >  2:571422/1‑140 (MQ=255)                                                          ctgcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAg                                                                            <  2:443353/138‑1 (MQ=255)                                                          cggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAg                                                                            <  1:1105894/140‑1 (MQ=255)                                                          cggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAg                                                                            <  2:1046006/140‑1 (MQ=255)                                                          cggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAg                                                                            <  2:962944/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATcc                                                                                         <  1:763206/126‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATcc                                                                                         >  2:763206/1‑126 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  2:374696/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  1:946701/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  2:982194/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  2:184428/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  1:878054/140‑1 (MQ=255)                                                           ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGt                                                                           <  1:446812/140‑1 (MQ=255)                                                            gcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTc                                                                          >  1:1123819/1‑140 (MQ=255)                                                                  ttAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATaa                                                                    <  1:571422/140‑1 (MQ=255)                                                                          ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATg                                                            >  1:297139/1‑140 (MQ=255)                                                                                 tCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATtgtg                                                     <  2:371335/140‑1 (MQ=255)                                                                                   cccAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  2:808528/139‑1 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   >  1:158911/1‑140 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  2:598243/140‑1 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  1:58599/140‑1 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  2:923548/140‑1 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   >  1:708324/1‑140 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  1:1027054/140‑1 (MQ=255)                                                                                   aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                   <  1:849456/140‑1 (MQ=255)                                                                                    ccAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGt                                                  <  2:59406/140‑1 (MQ=255)                                                                                         tGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTAt                                             <  2:446882/140‑1 (MQ=255)                                                                                               aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAat                                       >  2:234294/1‑140 (MQ=255)                                                                                                  aGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATc                                    >  1:802236/1‑140 (MQ=255)                                                                                                   gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCa                                   <  2:708324/140‑1 (MQ=255)                                                                                                                 cGACGGAAGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAAc                     <  1:1022186/140‑1 (MQ=255)                                                                                                                       aaGTCGTCTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGtt               >  2:241373/1‑140 (MQ=255)                                                                                                                           cgtcTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACa           <  1:84465/140‑1 (MQ=255)                                                                                                                             tcTTCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtt         >  1:659621/1‑140 (MQ=255)                                                                                                                                tCACTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTg      <  1:265241/140‑1 (MQ=255)                                                                                                                                  aCTCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGtt    <  1:642702/140‑1 (MQ=255)                                                                                                                                    tCCAGGCTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTcc  >  1:1049583/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ATTACACACCGAGATGACCTCTAACTCTTCTTCCAGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCC  >  NC_000913/2924837‑2925107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑