Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,825	A→T	I248N (ATC→AAC)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,825	0	A	T	100.0%	212.8 / NA	61	I248N (ATC→AAC)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (32/29);  total (32/29)

CAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGT  >  NC_000913/2941688‑2941958                                                                                                                                          |                                                                                                                                      cagcGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGttt                                                                                                                                     >  1:856912/1‑140 (MQ=255)         ttCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTg                                                                                                                             <  1:390224/140‑1 (MQ=255)                 gATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCa                                                                                                                     >  1:935850/1‑140 (MQ=255)                        tGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTAtt                                                                                                              >  2:177785/1‑140 (MQ=255)                        tGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTAtt                                                                                                              >  2:21208/1‑140 (MQ=255)                        tGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTAtt                                                                                                              >  1:27093/1‑140 (MQ=255)                          gCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTg                                                                                                            >  1:1021055/1‑140 (MQ=255)                           cGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGc                                                                                                           <  1:776118/140‑1 (MQ=255)                               aGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAgc                                                                                                       >  1:127031/1‑140 (MQ=255)                                           tACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGc                                                                                           <  2:856912/140‑1 (MQ=255)                                               cAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGt                                                                                       >  1:541515/1‑140 (MQ=255)                                                  ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGa                                                                                    >  1:892672/1‑140 (MQ=255)                                                  ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGa                                                                                    >  1:727538/1‑140 (MQ=255)                                                  ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGa                                                                                    >  1:224837/1‑140 (MQ=255)                                                     tGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAg                                                                                 <  2:727538/140‑1 (MQ=255)                                                       ccTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGcc                                                                               <  2:935850/140‑1 (MQ=255)                                                       ccTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGcc                                                                               >  2:184225/1‑140 (MQ=255)                                                            cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTg                                                                          >  2:552420/1‑140 (MQ=255)                                                            cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTg                                                                          <  1:544087/140‑1 (MQ=255)                                                            cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTg                                                                          >  2:544087/1‑140 (MQ=255)                                                            cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTg                                                                          >  2:879417/1‑140 (MQ=255)                                                               tCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTgcag                                                                       >  1:1011948/1‑140 (MQ=255)                                                                     cAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTgcag                                                                       >  1:907317/1‑134 (MQ=255)                                                                     cAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTgcag                                                                       <  2:907317/134‑1 (MQ=255)                                                                         ccAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGgcg                                                             >  1:948286/1‑140 (MQ=255)                                                                         ccAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGgcc                                                             >  1:112670/1‑139 (MQ=255)                                                                            gATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTa                                                          <  2:532709/140‑1 (MQ=255)                                                                                            aCTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGcc                                          <  1:177785/140‑1 (MQ=255)                                                                                             cTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGccc                                         <  2:224837/140‑1 (MQ=255)                                                                                                aCCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGc                                           <  2:293637/135‑1 (MQ=255)                                                                                                aCCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGc                                           >  1:293637/1‑135 (MQ=255)                                                                                                  cAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCt                                    >  2:365436/1‑140 (MQ=255)                                                                                                  cAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCt                                    >  2:376708/1‑140 (MQ=255)                                                                                                  cAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCt                                    <  1:757476/140‑1 (MQ=255)                                                                                                  cAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCt                                    <  2:1021055/140‑1 (MQ=255)                                                                                                    gAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGa                                  <  2:1011948/140‑1 (MQ=255)                                                                                                    gAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGa                                  <  1:21208/140‑1 (MQ=255)                                                                                                     aaCATCATTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGaa                                 <  1:376708/140‑1 (MQ=255)                                                                                                        atcatTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGt                              <  2:1155569/140‑1 (MQ=255)                                                                                                        atcatTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGt                              <  2:1004876/140‑1 (MQ=255)                                                                                                          catTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                            >  2:816259/1‑140 (MQ=255)                                                                                                          catTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                            >  1:501623/1‑140 (MQ=255)                                                                                                          catTAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGCTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                            >  1:881721/1‑140 (MQ=255)                                                                                                            ttAAACGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGat                          <  1:828422/140‑1 (MQ=255)                                                                                                               aaCGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATg                       >  1:178893/1‑140 (MQ=255)                                                                                                               aaCGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATg                       >  2:239331/1‑140 (MQ=255)                                                                                                                 cGGGCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAt                     <  2:127031/140‑1 (MQ=255)                                                                                                                    gCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGat                          <  2:319289/132‑1 (MQ=255)                                                                                                                    gCAAACAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGat                          >  1:319289/1‑132 (MQ=255)                                                                                                                      aaaCAAGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAc                >  2:961535/1‑140 (MQ=255)                                                                                                                          aaGACGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCa            <  2:511924/140‑1 (MQ=255)                                                                                                                              cGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg        >  1:707700/1‑140 (MQ=255)                                                                                                                              cGTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg        <  2:27093/140‑1 (MQ=255)                                                                                                                               gTCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt       <  2:707700/140‑1 (MQ=255)                                                                                                                                tCCGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTc      <  1:816259/140‑1 (MQ=255)                                                                                                                                  cGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg        >  2:576231/1‑136 (MQ=255)                                                                                                                                  cGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg        <  1:576231/136‑1 (MQ=255)                                                                                                                                  cGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt       >  1:528234/1‑137 (MQ=255)                                                                                                                                  cGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt       <  2:528234/137‑1 (MQ=255)                                                                                                                                  cGGCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCgg    >  2:726165/1‑140 (MQ=255)                                                                                                                                    gCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGt  <  2:1016875/140‑1 (MQ=255)                                                                                                                                    gCCTCGTTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGt  <  2:12599/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGT  >  NC_000913/2941688‑2941958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑