Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,147,044 T→C pseudogene (648/836 nt) yghX ← putative hydrolase fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,147,0440TC100.0% 188.4 / NA 53pseudogene (648/836 nt)yghXputative hydrolase fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

GCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCA  >  NC_000913/3146908‑3147177
                                                                                                                                        |                                                                                                                                     
gcggcAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTc                                                                                                                                    <  1:1050230/140‑1 (MQ=255)
       aTTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTcc                                                                                                                             >  2:290558/1‑140 (MQ=255)
          tGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCcgcg                                                                                                                          <  1:220137/140‑1 (MQ=255)
            tCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGaa                                                                                                                        <  1:662568/140‑1 (MQ=255)
             cATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAg                                                                                                                       <  2:326680/140‑1 (MQ=255)
              aTAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAg                                                                                                                       <  2:819920/139‑1 (MQ=255)
              aTAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAg                                                                                                                       >  1:819920/1‑139 (MQ=255)
                aaCGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTgg                                                                                                                    <  2:605138/140‑1 (MQ=255)
                aaCGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTgg                                                                                                                    <  2:1107189/140‑1 (MQ=255)
                 aCGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGa                                                                                                                   >  1:341982/1‑140 (MQ=255)
                   ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGagt                                                                                                                 <  1:660023/140‑1 (MQ=255)
                      gCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAg                                                                                                                       >  2:566759/1‑131 (MQ=255)
                      gCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAg                                                                                                                       <  1:566759/131‑1 (MQ=255)
                          ggAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAg                                                                                                          <  1:804055/140‑1 (MQ=255)
                                aTTATGGAATCCGTGATTAACTCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCtt                                                                                                    <  2:1059810/140‑1 (MQ=255)
                                      gAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTGCGCGAAGTGGAGTAGTAAAGGCGCTTGAATCt                                                                                              >  2:1029672/1‑140 (MQ=255)
                                         tCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCg                                                                                           >  1:576438/1‑140 (MQ=255)
                                         tCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCg                                                                                           >  1:88302/1‑140 (MQ=255)
                                          ccGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGc                                                                                          >  2:424321/1‑140 (MQ=255)
                                           cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCt                                                                                                     <  2:412307/128‑1 (MQ=255)
                                           cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCt                                                                                                     >  1:412307/1‑128 (MQ=255)
                                                  aaCCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCgg                                                                                  <  2:540874/140‑1 (MQ=255)
                                                   aCCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGc                                                                                 >  1:755303/1‑140 (MQ=255)
                                                   aCCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGc                                                                                 <  2:225058/140‑1 (MQ=255)
                                                                    cGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTgacgac                                                                >  2:54638/1‑140 (MQ=255)
                                                                     gCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGAAGTGGGTGCCTGACGAcc                                                               >  2:709507/1‑140 (MQ=255)
                                                                      ccTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCa                                                              >  1:414299/1‑140 (MQ=255)
                                                                       cTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGTCTGACGACCa                                                              <  2:951546/139‑1 (MQ=255)
                                                                       cTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGTCTGACGACCa                                                              >  1:951546/1‑139 (MQ=255)
                                                                       cTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCAt                                                             >  1:622813/1‑140 (MQ=255)
                                                                        tCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATa                                                            <  1:44102/140‑1 (MQ=255)
                                                                         cATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCTCCACATCGGCAGTg                                                                             >  1:424452/1‑122 (MQ=255)
                                                                         cATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCTCCACATCGGCAGTg                                                                             <  2:424452/122‑1 (MQ=255)
                                                                         cATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATaa                                                           >  1:890296/1‑140 (MQ=255)
                                                                          aTAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATaaa                                                          >  2:1007231/1‑140 (MQ=255)
                                                                                cTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCa                                                    <  2:890296/140‑1 (MQ=255)
                                                                                cTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCa                                                    <  2:341982/140‑1 (MQ=255)
                                                                                        tGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAg                                            <  2:1110725/140‑1 (MQ=255)
                                                                                         ggCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCCGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAgg                                           >  2:697599/1‑140 (MQ=255)
                                                                                          gCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGc                                          >  1:277697/1‑140 (MQ=255)
                                                                                          gCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGc                                          <  1:290558/140‑1 (MQ=255)
                                                                                          gCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGc                                          <  2:622813/140‑1 (MQ=255)
                                                                                          gCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGc                                          >  2:798088/1‑140 (MQ=255)
                                                                                          gCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGc                                          <  1:1007231/140‑1 (MQ=255)
                                                                                              tCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGt                                      >  1:12071/1‑140 (MQ=255)
                                                                                                 aCGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTcc                                   <  1:54638/140‑1 (MQ=255)
                                                                                                                 aGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccg                   <  2:414299/140‑1 (MQ=255)
                                                                                                                 aGGCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccg                   <  1:798088/140‑1 (MQ=255)
                                                                                                                   gCCAGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTCCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccgcc                 >  2:1004497/1‑140 (MQ=255)
                                                                                                                      aGCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCCGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCcgcg              <  1:697599/140‑1 (MQ=255)
                                                                                                                       gCCCTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGTCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGt             >  1:744263/1‑140 (MQ=255)
                                                                                                                        cccTCGTTGATTCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGtt            >  1:171759/1‑140 (MQ=255)
                                                                                                                                  ttCGGGCGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCa  >  2:367003/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCA  >  NC_000913/3146908‑3147177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑