Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,363	T→C	S492S (TCT→TCC)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,363	0	T	C	100.0%	314.0 / NA	86	S492S (TCT→TCC)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/42);  total (44/42)

ACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGT  >  NC_000913/3230227‑3230496                                                                                                                                         |                                                                                                                                      aCTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCggg                                                                                                                                    <  2:724166/140‑1 (MQ=255) aCTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCggg                                                                                                                                    <  1:846304/140‑1 (MQ=255)  cTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGc                                                                                                                                   >  1:1147459/1‑140 (MQ=255)     gtggtTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGa                                                                                                                                <  2:275864/140‑1 (MQ=255)     gtggtTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGa                                                                                                                                >  2:916844/1‑140 (MQ=255)     gtggtTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGa                                                                                                                                >  2:385330/1‑140 (MQ=255)        gtTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGaaca                                                                                                                             >  1:898278/1‑140 (MQ=255)               aaCCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGc                                                                                                                      >  1:644468/1‑140 (MQ=255)                aCCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGcc                                                                                                                     >  1:713909/1‑140 (MQ=255)                aCCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGcc                                                                                                                     >  2:41119/1‑140 (MQ=255)                  cgcgATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCcg                                                                                                                   <  1:605679/140‑1 (MQ=255)                    cgATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCcgcg                                                                                                                 >  2:818918/1‑140 (MQ=255)                    cgATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCcgcg                                                                                                                 <  2:644468/140‑1 (MQ=255)                      aTCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCgtg                                                                                                               >  1:134409/1‑140 (MQ=255)                             cggcaacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGgaga                                                                                                        >  2:1033652/1‑140 (MQ=255)                             cggcaacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGgaga                                                                                                        <  1:394350/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCAGAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  2:1087671/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  1:41119/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  1:818918/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  2:1110200/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  2:774146/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  2:898278/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  1:916844/140‑1 (MQ=255)                                caacggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACGGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGAAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAg                                                                                                     <  1:425171/140‑1 (MQ=255)                                  acggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGc                                                                                                   <  2:717396/140‑1 (MQ=255)                                  acggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGc                                                                                                   <  2:861174/140‑1 (MQ=255)                                  acggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGc                                                                                                   <  2:294102/140‑1 (MQ=255)                                  acggcGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGc                                                                                                   <  2:188261/140‑1 (MQ=255)                                        tGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTAc                                                                                             >  2:1070725/1‑140 (MQ=255)                                         gCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACg                                                                                            >  2:477654/1‑140 (MQ=255)                                         gCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACg                                                                                            <  1:1070725/140‑1 (MQ=255)                                          ccGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGa                                                                                           <  2:943229/140‑1 (MQ=255)                                                tatGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCt                                                                                     >  2:700231/1‑140 (MQ=255)                                                        gACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTc                                                                             >  1:1077316/1‑140 (MQ=255)                                                        gACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGACCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTc                                                                             >  1:898047/1‑140 (MQ=255)                                                         aCCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGa                                                                                  >  2:430886/1‑134 (MQ=255)                                                         aCCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGa                                                                                  <  1:430886/134‑1 (MQ=255)                                                             gcgACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGACCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGca                                                                        <  2:898047/140‑1 (MQ=255)                                                               gACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGcaca                                                                      <  1:980097/140‑1 (MQ=255)                                                                   aaGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGtt                                                                  >  1:1035398/1‑140 (MQ=255)                                                                    aGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTtg                                                                 >  2:44850/1‑140 (MQ=255)                                                                    aGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTtg                                                                 >  1:1136781/1‑140 (MQ=255)                                                                       ccACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTtgctg                                                              <  1:385330/140‑1 (MQ=255)                                                                           aacaCTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATc                                                          >  1:309104/1‑140 (MQ=255)                                                                           aacaCTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATc                                                          >  2:417462/1‑140 (MQ=255)                                                                            acacTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCg                                                         <  1:44850/140‑1 (MQ=255)                                                                                tGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTggg                                                     >  1:980952/1‑140 (MQ=255)                                                                                tGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTggg                                                     >  2:59071/1‑140 (MQ=255)                                                                                tGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTggg                                                     >  1:1115918/1‑140 (MQ=255)                                                                                tGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTggg                                                     >  1:811872/1‑140 (MQ=255)                                                                                  agagCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGaa                                                   <  2:1115918/140‑1 (MQ=255)                                                                                   gagCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAAt                                                  <  1:289688/140‑1 (MQ=255)                                                                                       ggcgAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCggg                                              >  2:26293/1‑140 (MQ=255)                                                                                       ggcgAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCggg                                              >  2:27997/1‑140 (MQ=255)                                                                                       ggcgAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCggg                                              >  1:586834/1‑140 (MQ=255)                                                                                       ggcgAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCggg                                              >  2:532312/1‑140 (MQ=255)                                                                                              tGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGtgat                                       >  2:707093/1‑140 (MQ=255)                                                                                              tGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGtgat                                       >  2:948961/1‑140 (MQ=255)                                                                                                     tACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccg                                <  1:1033652/140‑1 (MQ=255)                                                                                                     tACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccg                                <  2:829810/140‑1 (MQ=255)                                                                                                      aCGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccgc                               <  2:10708/140‑1 (MQ=255)                                                                                                        ggTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGccgccg                             >  1:519096/1‑140 (MQ=255)                                                                                                        ggTGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGccgccg                             >  2:187490/1‑140 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccgc                               >  1:764358/1‑136 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccgc                               <  2:764358/136‑1 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccg                                >  2:353716/1‑135 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccg                                <  1:353716/135‑1 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTc                           <  2:596003/140‑1 (MQ=255)                                                                                                          tGAAAAAAGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTc                           >  2:915687/1‑140 (MQ=255)                                                                                                             aaaaaGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCttt                        >  1:976832/1‑140 (MQ=255)                                                                                                             aaaaaGGCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCttt                        >  2:385365/1‑140 (MQ=255)                                                                                                                  ggCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGtt                   >  2:745994/1‑140 (MQ=255)                                                                                                                  ggCGATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGtt                   <  1:187490/140‑1 (MQ=255)                                                                                                                     gATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTAt                <  1:550234/140‑1 (MQ=255)                                                                                                                     gATAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTAt                <  2:976832/140‑1 (MQ=255)                                                                                                                       tAAAGAAGAGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCg              <  2:99149/140‑1 (MQ=255)                                                                                                                          agaagaGACGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACa           <  1:700231/140‑1 (MQ=255)                                                                                                                                 aCGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACa    >  2:733764/1‑140 (MQ=255)                                                                                                                                 aCGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACa    >  2:500835/1‑140 (MQ=255)                                                                                                                                 aCGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACa    >  1:712855/1‑140 (MQ=255)                                                                                                                                 aCGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACa    >  2:1114178/1‑140 (MQ=255)                                                                                                                                  cGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAg   <  1:417462/140‑1 (MQ=255)                                                                                                                                  cGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAg   <  2:811872/140‑1 (MQ=255)                                                                                                                                  cGCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAg   <  1:552685/140‑1 (MQ=255)                                                                                                                                   gCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGt  <  1:26293/140‑1 (MQ=255)                                                                                                                                   gCAGTCCGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGt  >  1:681128/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGT  >  NC_000913/3230227‑3230496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑