Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,864	C→T	A127A (GCC→GCT)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,864	0	C	T	100.0%	145.3 / NA	43	A127A (GCC→GCT)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/20);  total (23/20)

CTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTAT  >  NC_000913/3403736‑3403999                                                                                                                                 |                                                                                                                                        cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                                                                              <  1:827350/140‑1 (MQ=255)  tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                                                                              >  2:148417/1‑139 (MQ=255)  tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                                                                              <  1:148417/139‑1 (MQ=255)      tggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                                                                              <  2:964820/135‑1 (MQ=255)      tggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                                                                              >  1:964820/1‑135 (MQ=255)      tggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGc                                                                                                                         >  1:508011/1‑140 (MQ=255)      gggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGc                                                                                                                         <  2:442462/139‑1 (MQ=255)       ggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCt                                                                                                                        <  1:319882/140‑1 (MQ=255)        gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATg                                                                                                                          >  2:642042/1‑137 (MQ=255)        gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATg                                                                                                                          <  1:642042/137‑1 (MQ=255)             ggCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgtgtgt                                                                                                                  >  1:661210/1‑140 (MQ=255)              gCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgtgtgtg                                                                                                                 >  1:256090/1‑140 (MQ=255)               cGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGa                                                                                                                <  2:661210/140‑1 (MQ=255)               cGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGa                                                                                                                <  1:644709/140‑1 (MQ=255)                gTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGAt                                                                                                               >  1:1083245/1‑140 (MQ=255)                  tGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATgg                                                                                                             >  1:224291/1‑140 (MQ=255)                     tGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGgcgc                                                                                                          <  1:303501/140‑1 (MQ=255)                              cTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATg                                                                                                 >  2:859420/1‑140 (MQ=255)                               tGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTCCTGCTGGTTTTACCGCCATGCTGTGTGAGATGGCGCTGGAAGATGc                                                                                                <  2:264988/140‑1 (MQ=255)                               tGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGc                                                                                                >  1:1079438/1‑140 (MQ=255)                                      ggCTGGCGGAGCAGGCGCGGGTGAAAGGGGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTCCTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGCGtt                                                                                         >  2:739434/1‑140 (MQ=255)                                      ggCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGc                                                                                                >  2:326012/1‑133 (MQ=255)                                      ggCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGc                                                                                                <  1:326012/133‑1 (MQ=255)                                      ggCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGtt                                                                                         >  1:1031045/1‑140 (MQ=255)                                       gCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTc                                                                                        <  2:256090/140‑1 (MQ=255)                                           gCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCTGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGccc                                                                                    <  2:1079438/140‑1 (MQ=255)                                           gCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGccc                                                                                    >  1:1159301/1‑140 (MQ=255)                                                             aaaGGTGATGGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGt                                                                  <  2:224291/140‑1 (MQ=255)                                                                  tGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGa                                                             >  1:1113822/1‑140 (MQ=255)                                                                  tGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGa                                                             >  1:521152/1‑140 (MQ=255)                                                                        gctggTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTg                                                       >  1:443981/1‑140 (MQ=255)                                                                           ggTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCa                                                    <  2:1083245/140‑1 (MQ=255)                                                                              tGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAgtg                                                 >  1:19198/1‑140 (MQ=255)                                                                                   tGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGc                                            <  2:521152/140‑1 (MQ=255)                                                                                        cAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCgg                                       <  2:356435/140‑1 (MQ=255)                                                                                               tGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTAcc                                >  1:795478/1‑140 (MQ=255)                                                                                                   cgcgctTAAAGCAATAATTATCGGTACTGCTGGTTTTTCCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTAccgccg                            <  2:1031045/140‑1 (MQ=255)                                                                                                      gcgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTAccgccg                            <  2:685595/137‑1 (MQ=255)                                                                                                      gcgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTAccgccg                            >  1:685595/1‑137 (MQ=255)                                                                                                       cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGgc                        >  1:542708/1‑140 (MQ=255)                                                                                                       cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGgc                        >  2:678267/1‑140 (MQ=255)                                                                                                          aaaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCgct                     <  2:1159301/140‑1 (MQ=255)                                                                                                                 tGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCAt              <  2:443981/140‑1 (MQ=255)                                                                                                                  gATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATa             >  1:980065/1‑140 (MQ=255)                                                                                                                      aTCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGctgctg                 <  2:1000279/132‑1 (MQ=255)                                                                                                                      aTCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGctgctg                 >  1:1000279/1‑132 (MQ=255)                                                                                                                             ctgctgGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTAt  >  1:858757/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        CTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTAT  >  NC_000913/3403736‑3403999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑