Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,369	(T)6→5	intergenic (+39/+43)	bhsA → / ← ldtC	DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA/L,D‑transpeptidase YcfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,364	0	T	.	100.0%	160.3 / NA	38	intergenic (+34/+48)	bhsA/ldtC	DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA/L,D‑transpeptidase YcfS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/15);  total (23/15)

CAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTC  >  NC_000913/1169229‑1169495                                                                                                                                        |                                                                                                                                    cagcTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGAttttt                                                                                                                                 >  1:664281/1‑140 (MQ=255)   gcTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGAtttttcc                                                                                                                               <  1:14499/140‑3 (MQ=255)      ggCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCcg                                                                                                                           <  2:236787/140‑1 (MQ=255)        cgcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCcgcg                                                                                                                         <  2:664281/140‑1 (MQ=255)                             gcgcAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGAtt                                                                                                    >  1:123744/1‑140 (MQ=255)                                    aTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTAt                                                                                             <  1:1304856/140‑1 (MQ=255)                                      cTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATcc                                                                                           <  2:1068882/140‑1 (MQ=255)                                      cTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATcc                                                                                           <  2:1360186/140‑1 (MQ=255)                                             tATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt                                                                                     >  1:1143817/1‑139 (MQ=255)                                             tATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt                                                                                     <  2:1143817/139‑1 (MQ=255)                                                          aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                       >  2:831062/1‑140 (MQ=255)                                                              gTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                                   >  2:488881/1‑140 (MQ=255)                                                               tCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCa                                                                  >  1:1036430/1‑140 (MQ=255)                                                               tCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCa                                                                  >  1:4931/1‑140 (MQ=255)                                                                          tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcgg                                                       >  2:1471107/1‑140 (MQ=255)                                                                             aTGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                                    >  2:491927/1‑140 (MQ=255)                                                                             aTGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                                    >  1:939878/1‑140 (MQ=255)                                                                             aTGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                                    >  1:1153730/1‑140 (MQ=255)                                                                              tGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGa                                                   <  2:1153730/140‑1 (MQ=255)                                                                                gAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                 <  1:1471107/140‑1 (MQ=255)                                                                                       agTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGGTACTGCTGA‑TTTTTCCCTGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCCCCg                                          <  2:570101/140‑1 (MQ=255)                                                                                        gTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCgg                                         <  1:491927/140‑1 (MQ=255)                                                                                           aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                                      >  1:1337801/1‑140 (MQ=255)                                                                                                taaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCTCCGGCCTCCCgc                                 <  2:861698/140‑2 (MQ=255)                                                                                                taaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCgg                                 >  1:355594/1‑140 (MQ=255)                                                                                                    taaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg                             >  1:777649/1‑140 (MQ=255)                                                                                                    taaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg                             >  1:204959/1‑140 (MQ=255)                                                                                                      aGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                           >  2:899142/1‑140 (MQ=255)                                                                                                      aGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                           >  1:1246536/1‑140 (MQ=255)                                                                                                         aTTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcat                        <  2:4931/140‑1 (MQ=255)                                                                                                            aaCCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcca                     <  2:939878/140‑1 (MQ=255)                                                                                                             aCCCTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccat                    <  2:123744/140‑1 (MQ=255)                                                                                                                cTCATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGACATCCCGGAACGGACATCCATcac                 >  2:551834/1‑140 (MQ=255)                                                                                                                  cATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg                            <  2:443649/127‑1 (MQ=255)                                                                                                                  cATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg                            >  1:443649/1‑127 (MQ=255)                                                                                                                  cATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACAt               >  1:1217316/1‑140 (MQ=255)                                                                                                                  cATTAATGCCTGCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACAt               >  1:1172915/1‑140 (MQ=255)                                                                                                                             gCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt             <  2:726459/131‑1 (MQ=255)                                                                                                                             gCTACTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt             >  1:726459/1‑131 (MQ=255)                                                                                                                              cTACTGCTGA‑‑TTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTc  >  1:209650/1‑140 (MQ=255)                                                                                                                                aCTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt             >  2:946827/1‑128 (MQ=255)                                                                                                                                aCTGCTGA‑TTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt             <  1:946827/128‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTC  >  NC_000913/1169229‑1169495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑