Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,917,003	Δ1 bp	coding (746/1284 nt)	yebS →	intermembrane transport protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,917,001	0	C	.	100.0%	234.3 / NA	55	coding (744/1284 nt)	yebS	intermembrane transport protein YebS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/30);  total (25/30)

GTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGG  >  NC_000913/1916864‑1917135                                                                                                                                          |                                                                                                                                       gTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGccg                                                                                                                                      <  2:357516/140‑2 (MQ=255) gTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGccg                                                                                                                                      <  1:1189241/140‑2 (MQ=255)   gACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGccgcg                                                                                                                                    <  2:1382345/140‑4 (MQ=255)   gACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGccgcg                                                                                                                                    <  2:615530/140‑4 (MQ=255)      gTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTg                                                                                                                                <  2:961591/140‑1 (MQ=255)        tGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGcc                                                                                                                              <  1:455280/140‑1 (MQ=255)            acaTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCatat                                                                                                                          <  1:503812/140‑1 (MQ=255)            acaTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACACGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCatat                                                                                                                          <  1:1092111/140‑1 (MQ=255)                  tAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCt                                                                                                                    >  2:1328073/1‑140 (MQ=255)                  tAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCt                                                                                                                    <  2:546550/140‑1 (MQ=255)                    aTGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTAc                                                                                                                  >  2:1289610/1‑140 (MQ=255)                        cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCt                                                                                                              >  1:1399013/1‑140 (MQ=255)                         gaagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTg                                                                                                             >  1:1409304/1‑140 (MQ=255)                          aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCata                                                                                                                           <  1:1286229/125‑1 (MQ=255)                          aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCata                                                                                                                           >  2:1286229/1‑125 (MQ=255)                          aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGc                                                                                                            >  1:97220/1‑140 (MQ=255)                          aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGc                                                                                                            >  1:70327/1‑140 (MQ=255)                              aCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCg                                                                                                        <  2:1399013/140‑1 (MQ=255)                                 gtgGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCg                                                                                                     <  1:1328073/140‑1 (MQ=255)                                    ggAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCa                                                                                                  >  1:1122804/1‑140 (MQ=255)                                    ggAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCa                                                                                                  <  1:1263858/140‑1 (MQ=255)                                            tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGc                                                                                          >  1:260754/1‑140 (MQ=255)                                              tATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCaa                                                                                        <  1:649351/140‑1 (MQ=255)                                                  cGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAAt                                                                                    >  2:154558/1‑140 (MQ=255)                                                     aGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCt                                                                                 >  1:1255904/1‑140 (MQ=255)                                                     aGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCt                                                                                 >  2:1394869/1‑140 (MQ=255)                                                           ccGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGggcgg                                                                           <  1:1158523/140‑1 (MQ=255)                                                             gCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGgcggcg                                                                         <  2:187529/140‑1 (MQ=255)                                                                 cgcgTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGt                                                                     >  2:970712/1‑140 (MQ=255)                                                                        ggACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTc                                                              <  2:260754/140‑1 (MQ=255)                                                                                       tgtctgtcTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGtt                                               <  2:97220/140‑1 (MQ=255)                                                                                       tgtctgtcTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGtt                                               <  2:70327/140‑1 (MQ=255)                                                                                            gtctTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTg                                          >  1:651417/1‑140 (MQ=255)                                                                                             tctTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGc                                         >  1:465867/1‑140 (MQ=255)                                                                                              ctTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGcc                                        <  1:64327/140‑1 (MQ=255)                                                                                              ctTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGcc                                        <  1:489965/140‑1 (MQ=255)                                                                                              ctTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGcc                                        <  1:970712/140‑1 (MQ=255)                                                                                                    tGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTg                                  <  2:1122804/140‑1 (MQ=255)                                                                                                    tGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTg                                  <  2:651417/140‑1 (MQ=255)                                                                                                     gCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAAc                                     >  2:75448/1‑136 (MQ=255)                                                                                                     gCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAAc                                     <  1:75448/136‑1 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAAc                                     >  1:913269/1‑134 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAAc                                     <  2:913269/134‑1 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTg                               >  2:318783/1‑140 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTg                               <  2:654534/140‑1 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTg                               >  2:1393684/1‑140 (MQ=255)                                                                                                       cATTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTCCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGC‑AAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGc                              >  2:1083183/1‑140 (MQ=255)                                                                                                        aTTTTACCGGCTATGCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGc                              <  2:1507039/140‑1 (MQ=255)                                                                                                        aTTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGc                              <  2:465867/140‑1 (MQ=255)                                                                                                        aTTTTACCGGCTATCCAGATCAGCGTGGTCGCTG‑CCGCGTTCCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGC‑AAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGcc                             <  1:1083183/140‑1 (MQ=255)                                                                                                                    aTCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAATCTGTTGCCTATTTCTATCa                  <  1:1463994/140‑1 (MQ=255)                                                                                                                    aTCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCa                  <  1:499304/140‑1 (MQ=255)                                                                                                                    aTCCAGATCAGCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCa                  >  2:1113887/1‑140 (MQ=255)                                                                                                                              gCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTGTCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGaa        <  2:1409304/140‑1 (MQ=255)                                                                                                                              gCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGaa        <  2:372396/140‑1 (MQ=255)                                                                                                                              gCGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGaa        >  2:1259112/1‑140 (MQ=255)                                                                                                                               cGTGGTCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTAGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAAc       >  2:256477/1‑140 (MQ=255)                                                                                                                                    tCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACggagg  >  2:597031/1‑140 (MQ=255)                                                                                                                                    tCGCTG‑CCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACggagg  >  2:480479/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGG  >  NC_000913/1916864‑1917135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑