| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→10 | coding (492/732 nt) | pgeF ← | polyphenol oxidase YfiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 93.2% | 142.2 / 3.5 | 44 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (22/19); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| * | NC_000913 | 2,734,535 | 2 | . | C | 88.6% | 133.6 / 10.9 | 44 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (21/18); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCA > NC_000913/2734398‑2734663 || cgccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑cc < 1:395771/140‑1 (MQ=255) aaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑cccc‑‑ccccc < 1:1055461/140‑2 (MQ=255) aaCACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGaa < 1:836260/140‑1 (MQ=255) aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGt > 2:74880/1‑140 (MQ=255) gCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCCCCATGCTGAATGAAAGCTGCCCTTGCTTTAGCGTCTACTGCCATAAACCCCTCGCGACCCCCC‑‑‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCa < 1:604272/140‑1 (MQ=255) aGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑CCCC‑‑CCCCCACTCGAACGCGCGTGGACCAATTGc < 1:297771/140‑1 (MQ=255) gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTC‑‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGc < 1:1135929/140‑1 (MQ=255) aCGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGc < 1:378463/140‑1 (MQ=255) cGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTTCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGcc > 2:124152/1‑140 (MQ=255) ccGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCCCCCCC‑‑CCCCACTTCGAACGCGCGTGGTCCAATTGCCGGcc < 1:1478652/140‑1 (MQ=255) gCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc < 1:867209/140‑1 (MQ=255) aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt < 1:501877/140‑1 (MQ=255) tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa > 2:38534/1‑140 (MQ=255) tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa > 2:1472185/1‑140 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc > 2:1403968/1‑140 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc > 2:1159911/1‑140 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg > 1:550247/1‑140 (MQ=255) aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAc > 1:259770/1‑140 (MQ=255) aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAc > 1:880932/1‑140 (MQ=255) tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg > 2:187889/1‑140 (MQ=255) tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg > 2:1104011/1‑140 (MQ=255) gaaAGCTTCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt < 1:124152/140‑1 (MQ=255) gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt < 2:259770/140‑1 (MQ=255) aaaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc < 2:550247/140‑1 (MQ=255) aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑CCCCCACCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtctc > 2:1032373/1‑140 (MQ=255) gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct > 2:2332/1‑140 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTcc > 1:1071672/1‑140 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTcc > 2:1385110/1‑140 (MQ=255) ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:38534/140‑1 (MQ=255) ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:1472185/140‑1 (MQ=255) gCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACg > 1:5524/1‑140 (MQ=255) aGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTgc > 2:1516613/1‑140 (MQ=255) gTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:1159911/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 2:1071672/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:1403968/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:1385110/140‑1 (MQ=255) aCTGCCATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGt < 1:187889/140‑1 (MQ=255) cATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCAcc > 1:446937/1‑139 (MQ=255) cATAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg > 1:527095/1‑140 (MQ=255) aTAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc < 1:490752/140‑1 (MQ=255) aTAAACGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc < 1:1178160/140‑1 (MQ=255) aaaCGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCCCTTCGAACCCCCGGGGACCAATTCCCGGCCCTAACCAGGCGGAAATATTTTCCGGTTTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACCCCTCCCCACAGCCCACgcca > 2:497743/1‑140 (MQ=255) aaaCGCCTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGT‑CACgccag < 1:2332/140‑1 (MQ=255) cTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 2:932294/1‑140 (MQ=255) cTCGCGA‑‑ACCTCC‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 1:824963/1‑140 (MQ=255) cTCGCGA‑‑ACCTC‑‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa > 1:626284/1‑140 (MQ=255) || CGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCT‑‑‑CCCC‑‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCA > NC_000913/2734398‑2734663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |